BioMicroCenter:Nanostring: Difference between revisions
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== Nanostring nCounter System == | == Nanostring nCounter System == | ||
[[Image:BMC_Nanostring_System.jpg|Frame|thumb|200px|left|alt=none|The Nanostring nCounter System]] Starting next month, the [http://mit.edu/ki/index.html Koch Institute] and the MIT BioMicro Center will be hosting a [http://www.nanostring.com/ Nanostring] nCounter system on a trial basis. The [http://www.nanostring.com/products/ncounter/ nCounter system] is a [http://www.nanostring.com/applications/technology/ single molecule visualizer] that quantitatively detects RNA and other nucleic acids using a hybridization system in a highly | [[Image:BMC_Nanostring_System.jpg|Frame|thumb|200px|left|alt=none|The Nanostring nCounter System]] Starting next month, the [http://mit.edu/ki/index.html Koch Institute] and the MIT BioMicro Center will be hosting a [http://www.nanostring.com/ Nanostring] nCounter system on a trial basis. The [http://www.nanostring.com/products/ncounter/ nCounter system] is a [http://www.nanostring.com/applications/technology/ single molecule visualizer] that quantitatively detects RNA and other nucleic acids using a hybridization system in a highly multiplexed manner. The system uses color-coded molecular barcodes to digitally count nucleic acid molecules in solution. The system does not use enzymes for detecting the nucleic acids, allowing direct measurements of mRNA from a variety of input materials including degraded RNA or crude cell lysates. | ||
The instruments will be located in the BioMicro Center and will be available to all members of the MIT community. We will be using this time to gauge the level of interest in the nCounter and whether it would be a viable system to purchase. If you are interested in learning more, please contact [[BioMicroCenter:People|Stuart Levine]] | The instruments will be located in the BioMicro Center and will be available to all members of the MIT community. We will be using this time to gauge the level of interest in the nCounter and whether it would be a viable system to purchase. If you are interested in learning more, please contact [[BioMicroCenter:People|Stuart Levine]] | ||
Revision as of 15:48, 11 May 2010
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Nanostring nCounter System
Starting next month, the Koch Institute and the MIT BioMicro Center will be hosting a Nanostring nCounter system on a trial basis. The nCounter system is a single molecule visualizer that quantitatively detects RNA and other nucleic acids using a hybridization system in a highly multiplexed manner. The system uses color-coded molecular barcodes to digitally count nucleic acid molecules in solution. The system does not use enzymes for detecting the nucleic acids, allowing direct measurements of mRNA from a variety of input materials including degraded RNA or crude cell lysates.
The instruments will be located in the BioMicro Center and will be available to all members of the MIT community. We will be using this time to gauge the level of interest in the nCounter and whether it would be a viable system to purchase. If you are interested in learning more, please contact Stuart Levine
Code Sets
The Nanostring works by hybridizing the nucleic acid to a code set library. The "codes" are two-part oligos with ~50nt of homology each. The first oligo contains an anchoring sequence while the second contains ~7kb of fluorescently labeled nucleotides in a "barcode" that can be read by the imager. Code sets can include 24-500 different oligo pairs. These codesets, purchased from Nanostring, are the most expensive part of the process. Nanostring has created many virtual codesets (e.g. genes involved in angiogenesis or apoptosis)or can create custom code sets.
Logistics
Pricing and Schedules for the Nanostring are forthcoming. We anticipate the price of the run will be minimal ($10-$30 per sample) and your primary cost will be the Code Sets.
