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April 19, 2010 Starting next month, the Koch Institute and the MIT BioMicro Center will be hosting a Nanostring nCounter system on a trial basis. The nCounter system is a single molecule visualizer that quantitatively detects RNA and other nucleic acids using a hybridization system in a highly multiplex manner. The system uses color-coded molecular barcodes to digitally count nucleic acid molecules in solution. The system does not use enzymes for detecting the nucleic acids, allowing direct measurements of mRNA from a variety of input materials including degraded RNA or crude cell lysates. The instruments will be located in the BioMicro Center and will be available to all members of the MIT community. We will be using this time to gauge the level of interest in the nCounter and whether it would be a viable system to purchase. If you are interested in learning more, we will be hosting a seminar with Nanostring representatives on April 30th. The seminar will be at 11am in 68-181. Nanostring representatives will be available after the talk to discuss the instruments and specific applications. More information is in the ABSTRACT for the talk. April 1, 2010 Thanks to the generosity of a grant from the Moore Foundation to Dr. Penny Chisholm and ARRA funding to Dr. Chris Burge the BioMicro Center will be significantly expanding our sequencing capacity over the next 4-6 months. The first step of this expansion begins today with the addition of a new GAIIx and cBot from Illumina. We are working hard to get "Ryland" and "Agustus Gloop" up and running and we hope to be able to shorten our queue times very soon! March 2010 Due to an increase in the cost of Illumina reagents, increase in data file size, and the complexity of sample preps, we have had to increase, slightly, the cost of Illumina sequencing. These new rates will be effective on samples submitted after April 1, 2010. Samples already in the queue will use the current rates. ILLUMINA SEQUENCING MIT - current MIT - April 1, 2010 unit Notes Single End (36nt) $805 $860 per lane inlcudes quality control (RT-PCR or BioAnalyzer), sequencing, genome alignment and data storage of Firecrest files for 2 yrs. Paired End (36+36nt) $1,385 $1,450 per lane Add'l 36nt $295 $300 per lane Sample Multiplexing $0 $50 per 10 samples combined Combined over the whole order. Only applies to samples combined by BCM technicians. Repriming $200 $300 per flow cell Uses 6nt. Cost is divided by number of lanes requiring repriming. Repriming is required for standard Illumina multiplexing. Non-MIT rates are 30% higher for academic institutions and 50% highers for non-academic institutions. Priority is given to samples from CORE facilities.

RECENT & UPCOMING CHANGES Illumina/Solexa Sequencing -- *New* New Affymetrix labeling -- *New* Notes from the ABRF Tecan EVO high-throughput -- Training Feb 12, 2009 BioMicro Center Renovation -- Coming Feb '09 PREVIOUS NEWSLETTERS 2009 May January / February 2008 December November RECENT CHANGES TO THE WEBSITE <\BIG><\B> List of abbreviations: N This edit created a new page (also see list of new pages) m This is a minor edit b This edit was performed by a bot (±123) The page size changed by this number of bytes 16 April 2024      08:18  3D Printed Microfluidic Robots - Helen Hua‎‎ 2 changes history +6‎ [Michele Caggioni‎ (2×)]       08:18 (cur | prev) +22‎ Michele Caggioni talk contribs (→‎Actuation)       08:18 (cur | prev) −16‎ Michele Caggioni talk contribs (→‎Actuation)      08:11  3D Printing Overview‎ diffhist +422‎ Michele Caggioni talk contribs 15 April 2024      23:43  User:Yanbin Huang‎‎ 2 changes history +170‎ [Yanbin Huang‎ (2×)]       23:43 (cur | prev) 0‎ Yanbin Huang talk contribs (→‎Granted Patents)       23:43 (cur | prev) +170‎ Yanbin Huang talk contribs (→‎Granted Patents)      22:11  The paper that launched microfluidics - Xi Ning‎‎ 14 changes history +9,705‎ [Xning098‎ (14×)]       22:11 (cur | prev) −6‎ Xning098 talk contribs (→‎Summary)       22:07 (cur | prev) −12‎ Xning098 talk contribs (→‎Synthesis)       22:06 (cur | prev) 0‎ Xning098 talk contribs       22:06 (cur | prev) +1‎ Xning098 talk contribs       22:05 (cur | prev) 0‎ Xning098 talk contribs       22:03 (cur | prev) +630‎ Xning098 talk contribs       22:01 (cur | prev) +3,189‎ Xning098 talk contribs       21:44 (cur | prev) +688‎ Xning098 talk contribs (→‎Separation and quantification)       21:33 (cur | prev) +306‎ Xning098 talk contribs       21:29 (cur | prev) −2‎ Xning098 talk contribs (→‎Electrokinetic effect)       21:28 (cur | prev) −1‎ Xning098 talk contribs (→‎Separation and quantification)       21:27 (cur | prev) +398‎ Xning098 talk contribs (→‎Separation and quantification)       21:24 (cur | prev) +2,812‎ Xning098 talk contribs       21:06 (cur | prev) +1,702‎ Xning098 talk contribs      21:45  (Upload log)‎ [Xning098‎ (4×)]       21:45 Xning098 talk contribs uploaded File:Figure 4 Tdesign.png ‎       21:30 Xning098 talk contribs uploaded File:Figure 3 Set-up3.png ‎       21:24 Xning098 talk contribs uploaded File:Figure 2 Set-up1.png ‎       21:09 Xning098 talk contribs uploaded File:Figure 1 electroosmotic flow.png ‎ N    18:16  Multilayer Paper Microfluidics - Madyson Redder‎‎ 21 changes history +6,228‎ [Mredder‎ (21×)]       18:16 (cur | prev) +540‎ Mredder talk contribs (→‎Fabrication Methods)       18:07 (cur | prev) +822‎ Mredder talk contribs (→‎Fabrication Methods)       17:58 (cur | prev) +1,223‎ Mredder talk contribs (→‎Fabrication Methods)       17:47 (cur | prev) −47‎ Mredder talk contribs (→‎Motivation for Multilayer Paperfluidics)       17:46 (cur | prev) +2‎ Mredder talk contribs (→‎Advantages)       17:46 (cur | prev) +1,094‎ Mredder talk contribs (→‎Advantages)       17:37 (cur | prev) +24‎ Mredder talk contribs (→‎Materials)       17:37 (cur | prev) +619‎ Mredder talk contribs (→‎Materials)       17:19 (cur | prev) +18‎ Mredder talk contribs (→‎Uses)       17:19 (cur | prev) +7‎ Mredder talk contribs (→‎Uses)       17:18 (cur | prev) −19‎ Mredder talk contribs (→‎Developing Countries and Travel)       17:18 (cur | prev) +15‎ Mredder talk contribs (→‎Uses)       17:16 (cur | prev) 0‎ Mredder talk contribs (→‎Uses)       17:16 (cur | prev) +1,103‎ Mredder talk contribs (→‎Uses)       17:14 (cur | prev) −453‎ Mredder talk contribs (→‎Motivation for Multilayer Paperfluidics)       17:13 (cur | prev) +1‎ Mredder talk contribs (→‎Overview)       17:12 (cur | prev) +273‎ Mredder talk contribs (→‎Overview)       17:08 (cur | prev) −699‎ Mredder talk contribs (→‎Overview)       17:06 (cur | prev) +95‎ Mredder talk contribs       17:04 (cur | prev) +12‎ Mredder talk contribs N     17:03 (cur | prev) +1,598‎ Mredder talk contribs (Created page with "{{Template:CHEM-ENG590E}} Overview 3D polymeric or glass microfluidic devices were created to run tests on small amounts of liquid and receive results in a timely manner. However, these devices are costly and time consuming to produce. A solution to this problem was single-layer paper microfluidic devices. The most common known examples of single-layer paper microfluidic devices are pregnancy tests, COVID-19 antigen tests, and glucose test strips. While these devices a...")      17:02  CHEM-ENG590E:Wiki Textbook‎ diffhist +54‎ Mredder talk contribs (→‎Chapter 7 - Fiber-based Microfluidics)  m   07:22  Paper Microfluidic Device for Archiving Breast Epithelial Cells‎ diffhist +6‎ Sarah L. Perry talk contribs      06:39  Hu‎ diffhist +66‎ Hugangqing talk contribs 14 April 2024      19:57  Microfluidic Gradient Generators - Greg Schneider‎‎ 3 changes history +51‎ [Nmhatre‎ (3×)]       19:57 (cur | prev) +50‎ Nmhatre talk contribs (→‎Varying Flowrates and Asymmetrical Geometry)       19:57 (cur | prev) +473‎ Nmhatre talk contribs (→‎Varying Flowrates and Asymmetrical Geometry)       19:56 (cur | prev) −472‎ Nmhatre talk contribs (→‎Nonlinear Gradients)