BioMicroCenter:PippinPrep Testing: Difference between revisions
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{{BioMicroCenter}} | {{BioMicroCenter}} | ||
Evaluation of the [[BioMicroCenter:PippinPrep|BluePippin Prep:]] | == Evaluation of the [[BioMicroCenter:PippinPrep|BluePippin Prep:]] == | ||
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|'''Creating tight size distributions''' | |'''Creating tight size distributions''' | ||
| Here the Pippin prep was used to isolate tight bands from a broad distribution. Band sizes of +/-25bp can be isolated from 1.5% agarose cassettes. Tight size selection is useful in RNAseq and small RNAseq. | | Here the Pippin prep was used to isolate tight bands from a broad distribution. Band sizes of +/-25bp can be isolated from 1.5% agarose cassettes. Tight size selection is useful in RNAseq and small RNAseq. | ||
|[[Image:BMC_pippinTest2c.png|thumb|200px]] | |||
|[[Image:BMC_pippinTest2b.png|thumb|200px]] | |[[Image:BMC_pippinTest2b.png|thumb|200px]] | ||
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|'''How tight can the Blue Pippin go''' | |'''How tight can the Blue Pippin go''' | ||
Latest revision as of 10:27, 31 May 2012
| HOME -- | SEQUENCING -- | LIBRARY PREP -- | HIGH-THROUGHPUT -- | COMPUTING -- | OTHER TECHNOLOGY |
Evaluation of the BluePippin Prep:
| Isolation of individual bands | Here, the pippin prep has been used to isolate individual bands (lane 1-5) of a 50bp ladder (lane L). This isolation can be preprogrammed or based on peak position. Band isolation is useful for cloning and purification of PCR products. |  |
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| Creating tight size distributions | Here the Pippin prep was used to isolate tight bands from a broad distribution. Band sizes of +/-25bp can be isolated from 1.5% agarose cassettes. Tight size selection is useful in RNAseq and small RNAseq. |  |
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| How tight can the Blue Pippin go | Effect of band window on yield. To evaluate the importance of the window size on yield, we tested different sized windows on a standard Illumina library. We do notice significant decreases in yield when the window is smaller than 80bp without a comparable increase in resolution. |  |
