BioMicroCenter:PippinPrep Testing
From OpenWetWare
| HOME -- | SEQUENCING -- | LIBRARY PREP -- | HIGH-THROUGHPUT -- | COMPUTING -- | OTHER TECHNOLOGY |
Evaluation of the BluePippin Prep:
| Isolation of individual bands | Here, the pippin prep has been used to isolate individual bands (lane 1-5) of a 50bp ladder (lane L). This isolation can be preprogrammed or based on peak position. Band isolation is useful for cloning and purification of PCR products. |  |
 |
| Creating tight size distributions | Here the Pippin prep was used to isolate tight bands from a broad distribution. Band sizes of +/-25bp can be isolated from 1.5% agarose cassettes. Tight size selection is useful in RNAseq and small RNAseq. |  |
 |
| How tight can the Blue Pippin go | Effect of band window on yield. To evaluate the importance of the window size on yield, we tested different sized windows on a standard Illumina library. We do notice significant decreases in yield when the window is smaller than 80bp without a comparable increase in resolution. |  |
