BioMicroCenter:Pricing: Difference between revisions

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{{BioMicroCenter}}
{{BioMicroCenter}}
''' PRICING UPDATE 2/1/2023 '''
== SEQUENCING ==
== SEQUENCING ==
Please note that samples submitted from [[BioMicroCenter:CoreDeps|'''CORE LAB''']]s will be given priority on the HiSeq.
Please note that samples submitted from [[BioMicroCenter:CoreDeps|'''CORE LAB''']]s will be given priority on all equipment. The BioMicro Center reserves the right to reject samples for any reason. <BR><BR>


{| border=1  
=== NOVASEQ SEQUENCING ===
  ! ILLUMINA SEQUENCING
{| class="wikitable" border=1  
  ! NOVASEQ SEQUENCING
  !width=80| [[BioMicroCenter:CoreDeps|CORE LAB]]
  !width=80| [[BioMicroCenter:CoreDeps|CORE LAB]]
  !width=80| MIT
  !width=80| MIT
Line 11: Line 15:
  ! Notes
  ! Notes
  |-
  |-
  | colspan="6" align=center |<BR>'''HiSEQ 2000''' sequencing (minimum 150m reads/lane*. Average ~160-240m reads/lane)
  |NovaSeq SP 100nt
|align=right| $3,699
|align=right| $4,068
|align=right| $5,178
|rowspan=9| Per flowcell
|rowspan=9| Includes final pool quality control (RT-PCR and BioAnalyzer), sequencing, genome alignment and data storage of FASTQ files for 1 year
|-
|NovaSeq SP 300nt**
|align=right| $5,238
|align=right| $5,761
|align=right| $7,333.20
|-
|NovaSeq SP 500nt
|align=right| $7,020
|align=right| $7,722
|align=right| $9,828
|-
|NovaSeq S1 100nt**
|align=right| $5,832
|align=right| $6,415.20
|align=right| $8,164.80
|-
|NovaSeq S1 200nt**
|align=right| $7,452
|align=right| $8,197.20
|align=right| $10,432
|-
|NovaSeq S1 300nt**
|align=right| $8,127
|align=right| $8,939.70
|align=right| $11,377.80
|-
|NovaSeq S2 100nt**
|align=right| $10,395
|align=right| $11,434.50
|align=right| $14,553
|-
|NovaSeq S2 200nt**
|align=right| $12,960
|align=right| $14,256
|align=right| $18,144
|-
|NovaSeq S2 300nt**
|align=right| $13,500
|align=right| $14,850
|align=right| $18,900
|-
|NovaSeq S4 50nt
|align=right| $3,780
|align=right| $4,158
|align=right| $5,292
|rowspan=2| Per Lane
|rowspan=2| Includes final pool quality control (RT-PCR and BioAnalyzer), sequencing, genome alignment and data storage of FASTQ files for 1 year
|-
|NovaSeq S4 300nt
|align=right| $5,400
|align=right| $5,940
|align=right| $7,560
|-
|}
** - minimal inventory maintained
 
=== NEXTSEQ500 SEQUENCING ===
{| class="wikitable" border=1
! NEXTSEQ SEQUENCING
!width=80| [[BioMicroCenter:CoreDeps|CORE LAB]]
!width=80| MIT
!width=80| [[BioMicroCenter:FAQ#NON_MIT_USERS|Non-MIT]]
!width=80| unit
! Notes
  |-
  |-
  | Single End (40nt)
  |91nt kit <BR> (eg. 40PE or 75SE)
  |align="right"| $1,140
  |align="right"| $2,420
  |align="right"| $1,254
|align="right"| $2,662
  |align="right"| $1,710
|align="right"| $3,388
  |align="center"| per lane
|align="center" rowspan="3"| per flowcell
  | Includes final pool quality control (RT-PCR and BioAnalyzer), sequencing, genome alignment and data storage of BAM files for 1y.
|rowspan="3"| Includes final pool quality control (RT-PCR and BioAnalyzer), sequencing, genome alignment and data storage of FASTQ files for 1 year
|-
|166nt kit <BR> (eg. 80PE or 150SE)
|align="right"| $4,125
|align="right"| $4,537.50
|align="right"| $5,775
|-
|316nt kit <BR> (eg. 150PE or 300SE)
|align="right"| $6,270
|align="right"| $6,870
|align="right"| $8,778
|}
 
=== MISEQ SEQUENCING ===
{| class="wikitable" border=1
! MISEQ SEQUENCING
!width=80| [[BioMicroCenter:CoreDeps|CORE LAB]]
!width=80| MIT
!width=80| [[BioMicroCenter:FAQ#NON_MIT_USERS|Non-MIT]]
!width=80| unit
! Notes
|-
|70nt V2 kit <BR> (eg. 30PE or 70SE)
  |align="right"| $1,320
  |align="right"| $1,452
|align="right"| $1,848
  |align="center" rowspan=6| per lane
  |rowspan=6| Includes final pool quality control (RT-PCR and BioAnalyzer), sequencing, genome alignment and data storage of FASTQ files for 1 year
|-
|150nt V3 kits <BR> (eg. 75PE)
|align="right"| $1,430
|align="right"| $1,573
|align="right"| $2,002
|-
|300nt V2 kit<BR> (eg. 150PE)
|align="right"| $1,595
|align="right"| $1,754.50
|align="right"| $2,233
|-
|500nt V2 kit <BR> (eg. 250PE)
|align="right"| $1,760
|align="right"| $1,936
|align="right"| $2,464
|-
|600nt V3 kit<BR> (eg. 300PE)
|align="right"| $2,200
|align="right"| $2,420
|align="right"| $3,080
  |-
  |-
  | Paired End (40+40nt)
  |500nt '''NANO''' Run <BR> (eg. 250PE)
  |align="right"| $1,710
  |align="right"| $770
  |align="right"| $1,881
  |align="right"| $847
  |align="right"| $2,565
  |align="right"| $1,078
|align="center"| per lane
|}
  |  
 
=== [https://openwetware.org/wiki/BioMicroCenter:Singular_Sequencing#Requirements%2FThings_to_Consider SINGULAR G4] ===
{| class="wikitable" border=1
! SINGULAR G4 SEQUENCING
!width=80| [[BioMicroCenter:CoreDeps|CORE LAB]]
!width=80| MIT
  !width=80| [[BioMicroCenter:FAQ#NON_MIT_USERS|Non-MIT]]
!width=80| unit
! Notes
  |-
  |-
  | Add'l 40nt
  |50 PE: F3 Lane (~100m reads)
|align="right"| $300
  |align="right"| $330
  |align="right"| $330
  |align="right"| $363
  |align="right"| $420
|align="center" rowspan=2| per lane
|rowspan=3| Includes final pool quality control (RT-PCR and FA), sequencing, and data storage of FASTQ files for 1 year
|-
|150 PE: F3 Lane (~100m reads)
|align="right"| $450
  |align="right"| $495
  |align="right"| $495
  |align="center"| per lane
  |align="right"| $630
|
|-
| colspan="6" align=center |<BR>'''NextSeq''' sequencing (minimum 250m reads/lane. maximum ~500m reads/lane)
  |-
  |-
  | 91nt kit <BR> (eg. 40PE or 50SE)
  |Rapid 300nt: F3 flowcell (~400m reads)*
  |align="right"| $2,100
  |align="right"| $1800
  |align="right"| $2,310
  |align="right"| $1980
  |align="right"| $3,150
  |align="right"| $2520
  |align="center"| per flowcell
  |align="center"| per flowcell
| Includes final pool quality control (RT-PCR and BioAnalyzer), sequencing, genome alignment and data storage of BAM files for 1 year <BR> ** THESE RUNS WILL HAVE LOW PRIORITY
  |-
  |-
  | 166nt kit <BR> (eg. 80PE or 150SE)
|}
|align="right"| $3,150
  * Rapid flowcells are required for adapted Illumina libraries.
  |align="right"| $3,465
 
  |align="right"| $4,725
=== [https://openwetware.org/wiki/BioMicroCenter:Element_Sequencing ELEMENT AVITI] ===
  |align="center"| per flowcell
{| class="wikitable" border=1
  | Includes final pool quality control (RT-PCR and BioAnalyzer), sequencing, genome alignment and data storage of BAM files for 1 year
! ELEMENT AVITI SEQUENCING
!width=80| Lane/Flowcell
  !width=80| [[BioMicroCenter:CoreDeps|CORE LAB]]
!width=80| MIT
  !width=80| [[BioMicroCenter:FAQ#NON_MIT_USERS|Non-MIT]]
  !width=80| unit
  ! Notes
  |-
  |-
  | 316nt kit <BR> (eg. 150PE or 300SE)
  |rowspan=2| 75 PE
  |align="right"| $4,725
| Full flowcell (2 lane, 800m read)
  |align="right"| $5,200
  |align="right"| $1,900
  |align="right"| $7,088
  |align="right"| $2,090
  |align="right"| $2,660
  |align="center"| per flowcell
  |align="center"| per flowcell
  | Includes final pool quality control (RT-PCR and BioAnalyzer), sequencing, genome alignment and data storage of BAM files for 1 year
  |rowspan=5| Includes final pool quality control (RT-PCR and FA), sequencing, and data storage of FASTQ files for 1 year
|-
| colspan="6" align=center |<BR>'''MiSeq v2''' sequencing (minimum 8m reads/lane. maximum ~15m reads/lane)
  |-
  |-
  | 70nt Run <BR> (eg. 30PE or 70SE)
  |One lane (~400m read)
  |align="right"| $1,155
  |align="right"| $1,000
  |align="right"| $1,270
  |align="right"| $1,100
  |align="right"| $1,732
  |align="right"| $1,400
  |align="center"| per lane
  |align="center"| per lane
| Includes final pool quality control (RT-PCR and BioAnalyzer), sequencing, genome alignment and data storage of BAM files for 1 year
  |-
  |-
  | 300nt Run <BR> (eg. 150PE)
  |rowspan=2| 150 PE
  |align="right"| $1,365
| Full flowcell (2 lane, ~800m read)
  |align="right"| $1,500
  |align="right"| $2,650
  |align="right"| $2,050
  |align="right"| $2,915
  |align="center"| per lane
  |align="right"| $3,710
| Includes final pool quality control (RT-PCR and BioAnalyzer), sequencing, genome alignment and data storage of BAM files for 1 year
  |align="center"| per flowcell
  |-
  |-
  | 500nt Run <BR> (eg. 250PE)
  |One lane (~400m read)
  |align="right"| $1,575
  |align="right"| $1,375
  |align="right"| $1,732
  |align="right"| $1,513
  |align="right"| $2,362
  |align="right"| $1,925
  |align="center"| per lane
  |align="center"| per lane
| Includes final pool quality control (RT-PCR and BioAnalyzer), sequencing, genome alignment and data storage of BAM files for 1 year
|-
| colspan="6" align=center |<BR>'''MiSeq v3''' sequencing (minimum 15m reads/lane. Expected ~25m reads/lane)
  |-
  |-
  | 150nt Run <BR> (eg. 75PE)
  | 300 PE
  |align="right"| $1,365
| Full flowcell (~200m reads)
  |align="right"| $1,500
  |align="right"| $3,500
  |align="right"| $2,050
  |align="right"| $3,850
  |align="right"| $4,900
  |align="center"| per lane
  |align="center"| per lane
| Includes final pool quality control (RT-PCR and BioAnalyzer), sequencing, genome alignment and data storage of BAM files for 1 year
|}
|-
Pricing effective 12/1/2023
| 600nt Run <BR> (eg. 300PE)
 
|align="right"| $1,890
=== OTHER SHORT READ SERVICES ===
  |align="right"| $2,080
{| class="wikitable" border=1  
  |align="right"| $2,835
  ! ILLUMINA SUPPORTING SERVICES
  |align="center"| per lane
  !width=80| [[BioMicroCenter:CoreDeps|CORE LAB]]
  | Includes final pool quality control (RT-PCR and BioAnalyzer), sequencing, genome alignment and data storage of BAM files for 1 year
  !width=80| MIT
|-
  !width=80| [[BioMicroCenter:FAQ#NON_MIT_USERS|Non-MIT]]
  | colspan="6" align=center |<BR>'''Additional Services'''
  !width=80| unit
|-
  ! Notes
  | colspan="6" align=center |''These services apply to samples submitted for all platforms.''
  |-
  |-
  | Quality Control Only -only one QC is included per sequencing lane.
  | Quality Control Only -only one QC is included per sequencing lane.
  |align="right"| $20
  |align="right"| $25
  |align="right"| $26
  |align="right"| $27.50
  |align="right"| $30
  |align="right"| $35
  |align="center"| per sample
  |align="center"| per sample
  | Includes RT-PCR and Frag.Analyzer
  | Includes RT-PCR and Frag.Analyzer
  |-
  |-
  | Rapid Quality Control - Use SYBR for sample quantification for pooling instead of qPCR. Only available for pooling.
  | Rapid Quality Control - Use SYBR for sample quantification for pooling instead of qPCR. Only available for pooling.
  |align="right"| $10
  |align="right"| $12
  |align="right"| $13
  |align="right"| $13.20
  |align="right"| $15
  |align="right"| $16.80
  |align="center"| per sample
  |align="center"| per sample
  | Includes Fluorometric quantification and Frag.Analyzer
  | Includes Fluorometric quantification and Frag.Analyzer
|-
|qPCR only (short read)
|align="right"| $18
|align="right"| $19.80
|align="right"| $25.20
|align="center"| per sample
| Includes qPCR values from standard QC only
|-
|qPCR only - full plate(short read)
|align="right"| $330
|align="right"| $363
|align="right"| $462
|align="center"| per sample
| excludes column 1 (used for controls)
|}
|}


<BR><BR>
=== LONG READ SEQUENCING ===
Please note that samples submitted from [[BioMicroCenter:CoreDeps|'''CORE LAB''']]s will be given priority on all equipment. The BioMicro Center reserves the right to reject samples for any reason.''<BR>
 
=== SAMPLE PREPARATION - STANDARD ===


{| border=1  
{| class="wikitable" border=1  
  ! ILLUMINA Sample Preparation - STANDARD
  ! SEQUEL SEQUENCING
  !width=80| [[BioMicroCenter:CoreDeps|CORE LAB]]
  !width=80| [[BioMicroCenter:CoreDeps|CORE LAB]]
  !width=80| MIT
  !width=80| MIT
Line 128: Line 270:
  ! Notes
  ! Notes
  |-
  |-
  | colspan="6" | '''STANDARD THROUGHPUT DNA''' <BR> Prices for individual samples. Repreps will typically be attempted on failed samples without charge.
  | 20 hour v3 Chemistry
|align="right"| $1,470
|align="right"| $1,617
|align="right"| $2,205
|align="center"| per SMRTCell
|align="center"| Includes polymerase binding and movie.
 
  |-
  |-
  | NEOPREP DNA <BR> * Neoprep will be retired 7/17
  ! PROMETHION SEQUENCING
  |align="right"| $70
  !width=80| [[BioMicroCenter:CoreDeps|CORE LAB]]
  |align="right"| $77
  !width=80| MIT
  |align="right"| $105
  !width=80| [[BioMicroCenter:FAQ#NON_MIT_USERS|Non-MIT]]
  |align="center"| per sample
  !width=80| unit
  | Includes adapter ligation, barcoding, enrichment, and quality control (RT-PCR and BioAnalyzer). Insert sizes above 250bp.
  ! Notes
  |-
  |-
  | Full Service Sheared DNA (SPRIWORKS)
  | 72 hour Flowcell
  |align="right"| $130
  |align="right"| $1,200
  |align="right"| $143
  |align="right"| $1,320
  |align="right"| $195
  |align="right"| $1,680
  |align="center"| per sample
  |align="center"|per Flowcell
  | Includes adapter ligation, size selection, barcoding, enrichment, and quality control (RT-PCR and BioAnalyzer).
  ||Fastq stored for 1y. Trace data stored only 30d.
|}
 
== LIBRARY GENERATION ==
=== SHORT READ LIBRARY - CONVERSION ===
{| class="wikitable" border=1
!width=100| Input library
!width=100| Convert To
!width=100| Method
!width=80| [[BioMicroCenter:CoreDeps|CORE LAB]]
!width=80| MIT
!width=80| [[BioMicroCenter:FAQ#NON_MIT_USERS|Non-MIT]]
! Notes
  |-
  |-
  | ''SPRI-TE use only''
  |align="center"|Illumina
  |align="right"| $85
|align="center"|Singular
  |align="right"| $95
  |align="center"|Index Overwrite (per sample)
  |align="right"| N/A
  |align="right"| $30
  |align="center"| per sample
  |align="right"| $33
  | User must provide all primers. Samples will be returned for amplification.
  |align="right"| $42
  | Includes QC and reindexing
  |-
  |-
| Intact Genomic DNA (NEXTERA) - 50ng input
  |align="center"|Illumina
  |align="right"| $150
  |align="center"|Singular
  |align="right"| $165
  |align="center"|P5/P7 conversion (per pool)
|align="right"| $225
  |align="center"| per sample
| Includes clean up, tagmentation, barcoding, enrichment, and quality control (RT-PCR and BioAnalyzer).
|-
| Low Input Intact Genomic DNA (NEXTERA-XT) - 1ng input
  |align="right"| $100
  |align="right"| $100
  |align="right"| $110
  |align="right"| $110
  |align="right"| $150
  |align="right"| $140
|align="center"| per sample
  | Includes QC. Samples not reindexed <BR><B> Requires full flowcell for sequencing (Rapid) </B>
  | Includes tagmentation, barcoding, enrichment, and quality control (RT-PCR and BioAnalyzer).
  |-
  |-
  | colspan="6" | '''STARTING WITH RNA'''<BR>Prices for individual samples. Repreps will typically be attempted on failed samples without charge.
  |align="center"|Illumina
|align="center"|Element
|align="center"|Index Overwrite (per sample)
|align="right"| $30
|align="right"| $33
|align="right"| $42
| Includes QC and reindexing - "Elevate" workflow
  |-
  |-
  | Illumina NEOPREP <BR> * Neoprep will be retired 7/17
  |align="center"|Illumina
|align="center"|Element
|align="center"|P5/P7 splicing (per pool)
  |align="right"| $100
  |align="right"| $100
  |align="right"| $110
  |align="right"| $110
  |align="right"| $150
  |align="right"| $140
| Includes QC. Samples not reindexed. Requires high input amounts (>10nM)
|}
 
=== SHORT READ LIBRARY - DNA ===
 
{| class="wikitable" border=1
!width=160| ILLUMINA Sample Preparation
!width=60| Type
!width=60| Batch Size
!width=80| [[BioMicroCenter:CoreDeps|CORE LAB]]
!width=80| MIT
!width=80| [[BioMicroCenter:FAQ#NON_MIT_USERS|Non-MIT]]
! Notes
|-
! colspan="4" | '''STANDARD THROUGHPUT DNA''' <BR> Prices for individual samples. Repreps will typically be attempted on failed samples without charge.
! colspan="3" style="color: blue;" | '''HIGH THROUGHPUT DNA''' <BR> Prices for sample batches. Repreps will NOT be attempted on failed samples without charge. Low volume (<=5ul) submission must be coordinated with BMC staff. Initial QC not included.
|-
|rowspan=3 |LIGATION BASED<BR>NEB UltraII
|align="center"|STD
  |align="center"| per sample
  |align="center"| per sample
  | Performed using Neoprep system. <br> Requires polyA+ total RNA as input. No other inputs allowed. <BR>Includes purifying mRNA, generation of fragmented dsDNA, adaptor ligation, size selection, barcoding, enrichment, and quality control (RT-PCR and BioAnalyzer)
  |align="right"| $105
|align="right"| $115
|align="right"| $157
| Includes QC, adapter ligation, size selection, barcoding, enrichment, and quality control (SYBR and FA).
|- style="color: blue;"
|align="center" rowspan=2|HTL
|align="center"| 24
|align="right"| $825
|align="right"| $907.50
|align="right"| $1,155
|rowspan=2 | Includes adapter ligation, size selection, barcoding, enrichment, and quality control spotcheck (SYBR and FA).
|- style="color: blue;"
|align="center"| 96
|align="right"| $1,625
|align="right"| $1,787.50
|align="right"| $2,275
|-
 
|Tagmentation <BR> NEXTERA-XT
|align="center" rowspan=2|STD
|align="center" rowspan=2|per Sample
|align="right"| $105
|align="right"| $115
|align="right"| $157
| rowspan="2" | Includes set-up, tagmentation, indexing, enrichment, and quality control (SYBR and FA).  
|-
|Tagmentation <BR> NEXTERA-FLEX
|align="right"| $157
|align="right"| $173
|align="right"| $236
|- style="color: blue;"
|rowspan=2 | NEXTERA-XT OR FLEX
|rowspan=2 align="center"| HTL
|align="center"| ''24''
|align="right"| $825
|align="right"| $907.50
|align="right"| $1,155
|rowspan=2 | Includes tagmentation, indexing, enrichment, and quality control spotcheck(SYBR and FA).
|-
|align="center" style="color: blue;" | ''96''
|align="right" style="color: blue;" | $1,625
|align="right" style="color: blue;" | $1,787.50
|align="right" style="color: blue;" | $2,275
|-style="color: blue;"
|rowspan="2"  | 16S Amplicon
|align="center" rowspan=2 | HTL
|align="center"|48
|align="right" | $1,900
|align="right" | $2,090
|align="right" | $2,660
|rowspan="2"  | Includes normalization, enrichment, pooling and quality control (SYBR and FA).
|-style="color: blue;"
|align="center"|add'l 48
|align="right" | $650
|align="right" | $715
|align="right" | $910
 
|-style="color: blue;"
|rowspan="2"  | Other Amplicon
|rowspan=2 align="center"| HTL
|align="center" |48
|align="right" | $1,600
|align="right" | $1,760
|align="right" | $2,240
|rowspan="2"  | Includes normalization, enrichment, pooling and quality control (SYBR and FA). <BR> Samples *must* be submitted amplified with appropriate linkers.
|- style="color: blue;"
|align="center"|add'l 48
|align="right" | $630
|align="right" | $693
|align="right" | $945
|-
! colspan="7" | '''ADDITIONAL FEES''' <BR> Samples not in proper input format will be subject to these additional charges.
|-
|style="color: blue;" colspan=2| High Throughput Setup
|align="center" style="color: blue;" |48 samples
|align="right" style="color: blue;" | $50
|align="right" style="color: blue;" | $55
|align="right" style="color: blue;" | $75
|
|-
|colspan=2|Sample Cleanup
|align="center"|per sample
|align="right"| $5
|align="right"| $5.50
|align="right"| $7
|SPRI cleanup of samples to remove impurities.
  |-
  |-
  |rowspan="2" | Kapa mRNA Hyperprep
  |style="color: blue;" colspan=2|Quantification
  |align="right"| $150
|align="center" style="color: blue;" |per sample
  |align="right"| $165
  |align="right" style="color: blue;" | $6
  |align="right"| $225
  |align="right" style="color: blue;" | $6.60
  |align="right" style="color: blue;" | $8.40
|Quantification of samples (FA) prior to arraying.
|}
 
=== SHORT READ LIBRARY - RNA ===
 
{| class="wikitable" border=1
!width=160| ILLUMINA Sample Preparation
!width=60| Type
!width=60| Batch Size
!width=80| [[BioMicroCenter:CoreDeps|CORE LAB]]
!width=80| MIT
!width=80| [[BioMicroCenter:FAQ#NON_MIT_USERS|Non-MIT]]
! Notes
|-
! colspan="4" | '''STANDARD RNA''' <BR> Prices for individual samples. Repreps will typically be attempted on failed samples without charge.
! colspan="3" style="color: blue;" | '''HIGH THROUGHPUT RNA''' <BR> Repreps will NOT be attempted on failed samples without charge. Low volume (<=5ul) submission must be coordinated with BMC staff. Initial QC not included
|-
|rowspan="3"|polyA based isolation <BR><BR> NEB UltraII RNAseq
|align="center"|<B>STD</B>
  |align="center"| per sample
  |align="center"| per sample
  |rowspan="2" | Includes QC, polyA isolation, generation of fragmented dsDNA, adaptor ligation, indexing, enrichment, and quality control (RT-PCR and BioAnalyzer).
  |align="right"| $157
|align="right"| $173
|align="right"| $236
|Includes QC, polyA isolation, generation of cDNA, library construction, indexing and quality control (SYBR + FA).
|-
|rowspan=2 align="center" style="color: blue;"| <B>HTL</b><BR>(NEB only)
|align="center" style="color: blue;" | ''24''
|align="right" style="color: blue;" | $1,400
|align="right" style="color: blue;" | $1,540
|align="right" style="color: blue;" | $1,960
|Includes polyA isolation, generation of cDNA, library construction, indexing and spot QC (SYBR + FA).
|-
|align="center" style="color: blue;" | ''96''
|align="right" style="color: blue;" | $2,800
|align="right" style="color: blue;" | $3,080
|align="right" style="color: blue;" | $3,920
|Includes polyA isolation, generation of cDNA, library construction, indexing and spot QC (SYBR + FA).
  |-
  |-
  |align="right"| $2000
  |align="right"| $2200
|rowspan="2"| High Throughput 3' Digital Gene Expression (HT3DGE)
  |align="right"| $3000
|rowspan=2  align="center" style="color: blue;"| <B>HTL</b>
  |align="center"| per 24 sample <br> TECAN
|align="center" style="color: blue;"| ''24''
  |align="right" style="color: blue;" | $1,050
  |align="right" style="color: blue;" | $1,155
  |align="right" style="color: blue;" | $1,470
  | rowspan="2"  style="color: blue;" | Includes generation of cDNA, library construction, indexing and library quality control. Does not include sample QC.
  |-
  |-
  | Epicenter Ribozero<br>''no polyA pulldown''
  |align="center" style="color: blue;" | ''96''
  |align="right"| $350
  |align="right" style="color: blue;" | $2,100
  |align="right"| $385
  |align="right" style="color: blue;" | $2,310
  |align="right"| $525
  |align="right" style="color: blue;" | $2,940
|-
|rowspan="3"|Total RNA sequencing <BR><BR> NEB RNAseq +Ribodepletion
|align="center"|<B>STD</B>
  |align="center"| per sample
  |align="center"| per sample
  | Requires 1ug of RNA. Based on Illumina Truseq chemistry
  |align="right"| $235
|align="right"| $258.5
|align="right"| $352.5
|Includes sample QC, ribosomal depletion, generation of cDNA, library construction, indexing and library quality control.
|-
|rowspan=2 align="center" style="color: blue;"| <B>HTL</b><BR>(NEB only, covaris RNA fragmentation)
|align="center" style="color: blue;" | ''24''
|align="right" style="color: blue;" | $2,400
|align="right" style="color: blue;" | $2,640
|align="right" style="color: blue;" | $3,360
|rowspan=2|Includes polyA isolation, generation of cDNA, library construction, indexing and spot QC (SYBR + FA).
|-
|align="center" style="color: blue;" | ''96''
|align="right" style="color: blue;" | $4,800
|align="right" style="color: blue;" | $5,280
|align="right" style="color: blue;" | $6,720
|-
|rowspan=3|Low Input polyA+ <BR> Clontech SMARTseq + NexteraXT or Flex
|align="center"| '''STD'''<BR>v4
|align="center"|per sample
|align="right"| $262
|align="right"| $289
|align="right"| $394
| rowspan="3" | Includes generation of cDNA, amplification, library construction, indexing and quality control (SYBR + FA). Single sample includes initial QC.
  |-
  |-
  | Low Input (Clontech)
  |rowspan="2" align="center" style="color: blue;"| '''HTL'''<br>v2
  |align="right"| $375
|align="center" style="color: blue;" | ''24''
  |align="right"| $412.5
|align="right" style="color: blue;" | $1880
  |align="right"| $562.5
|align="right" style="color: blue;" | $1980
|align="right" style="color: blue;" | $2520
|-
|align="center" style="color: blue;" | ''96''
  |align="right" style="color: blue;" | $2800
  |align="right" style="color: blue;" | $3080
  |align="right" style="color: blue;" | $3920
|-
|rowspan="3"|Low Input Mammalian Total <BR> Clontech ZapR Kit
|align="center"| STD
  |align="center"| per sample
  |align="center"| per sample
  | Can work on single cells
  |align="right"| $210
|align="right"| $231
|align="right"| $315
| rowspan="1" | Includes generation of cDNA, amplification, library construction, rRNA degradation, indexing and quality control (SYBR + FA).
  |-
  |-
  | Low Input (NuGen)
  |rowspan=2 align="center" style="color: blue;"| <B>HTL</b><BR>(covaris RNA fragmentation)
  |align="right"| $430
  |align="center" style="color: blue;" | ''24''
  |align="right"| $473
  |align="right" style="color: blue;" | $2200
  |align="right"| $645
  |align="right" style="color: blue;" | $2420
  |align="center"| per sample
  |align="right" style="color: blue;" | $3080
  | Can work on degraded RNA.
  |rowspan=2|Includes polyA isolation, generation of cDNA, library construction, indexing and spot QC (SYBR + FA).
  |-
  |-
| Tight Size Cut
  |align="center" style="color: blue;" | ''96''
  |align="right"| $85
  |align="right" style="color: blue;" | $4400
  |align="right"| $94
  |align="right" style="color: blue;" | $4840
  |align="right"| $130
  |align="right" style="color: blue;" | $6160
  |align="center"| per pool
| +/- 40bp using PippinPrep
  |-
  |-
  | Small RNA
  | Small RNA
  | align="right"|$275
  |align="center"| STD
| align="right"|$303
| align="right"|$413
  | align="center"|per sample
  | align="center"|per sample
  | Uses NEB small RNA kit
| align="right"|$289
| align="right"|$318
| align="right"|$433
  | Uses NEB, BIOO or QIAGEN small RNA kit
  |-
  |-
  | colspan="6" | '''STARTING WITH CHROMATIN''' Note: User must supply antibodies.
  ! colspan="7" | '''ADDITIONAL FEES''' <BR> Samples not in proper input format will be subject to these additional charges.
  |-
  |-
  | Full Service ChIP-seq
  |colspan=2 style="color: blue;" | High Throughput Setup
  |align="right"| $210
  |align="center" style="color: blue;" |48 samples
  |align="right"| $231
  |align="right" style="color: blue;" | $50
  |align="right"| $315
  |align="right" style="color: blue;" | $55
  |align="center"| per sample
  |align="right" style="color: blue;" | $75
  | Performed using IP-Star AND SPRIworks system. <br> Includes ChIP, library contruction and barcoding.
  |  
  |-
  |-
  | ''IP-Star Only''
  |colspan=2|Sample Cleanup
  |align="right"| $300
  |align="center"|per sample
  |align="right"| $330
  |align="right"| $5
  |align="right"| $450
  |align="right"| $5.50
  |align="center"| per batch (1-8 samples)
  |align="right"| $7.50
  | Performed using IP-Star
  |SPRI cleanup of samples to remove impurities.
  |-
  |-
  | Covaris Sonicator
  |colspan=2 style="color: blue;" |Quantification
  |align="right"| $15
  |align="center" style="color: blue;" |per sample
  |align="right"| $20
  |align="right" style="color: blue;" | $6
  |align="right"| NA
  |align="right" style="color: blue;" | $6.60
  |align="center"| per hour
  |align="right" style="color: blue;" | $8.40
  | Training Required
  |Quantification of samples (FA) prior to arraying.
  |-
  |-
|}
|}
Line 248: Line 597:
.
.


=== SAMPLE PREPARATION - HIGH THROUGHPUT ===
=== LONG READ LIBRARIES ===
 
{| class="wikitable" border=1
{| border=1  
  ! PacBio Sample Preparation
  ! ILLUMINA Sample Preparation - HIGH-THROUGHPUT
!width=120| Batch Size
  !width=80| [[BioMicroCenter:CoreDeps|CORE LAB]]
  !width=80| [[BioMicroCenter:CoreDeps|CORE LAB]]
  !width=80| MIT
  !width=80| MIT
  !width=80| [[BioMicroCenter:FAQ#NON_MIT_USERS|Non-MIT]]
  !width=80| [[BioMicroCenter:FAQ#NON_MIT_USERS|Non-MIT]]
!width=80| Batch size
!width=160| INPUT REQUIREMENT
  ! Notes
  ! Notes
  |-
  |-
  | colspan="7" | '''LIBRARY PREP''' <BR> Repreps will NOT be attempted on failed samples without charge. Submission must be coordinated with BMC staff.
  |rowspan=2|SMRTBell Only<BR> eg:PCR products
| Sample 1 || $315 || $346 || $472
|rowspan=2| Includes QC, repair and addition of indexed SMRTBells
  |-
  |-
  | AUTOMATED NEXTERA-XT - 1ng input
  | Samples 2-8 || $157 || $173 || $236
|align="right"| $1000
|align="right"| $1100
|align="right"| $1500
|align="center"| 16 samples <BR>(2 column)
|>7ul at 0.2ng/ul in Axygen plate
| Includes tagmentation, barcoding, enrichment, and quality control (RT-PCR and BioAnalyzer). May have 10% failure rate.
  |-
  |-
  | MOSQUITO NEXTERA-XT - 100pg input (96/384)
  |rowspan=2|Genomic Library
  |align="right"| $1500 / $3000
  | Sample 1 || $472 || $520 || $709
|align="right"| $1650 / $3300
  |rowspan=2| Includes Covaris gSPIN fragmentation, Pippin sizing, repair and SMRTbell as above
|align="right"| $2250 / $4500
  |align="center"| 96 / 384
|>5ul at 0.2ng/ul in Axygen plate
| Includes tagmentation, barcoding, enrichment, and picogreen quality control. RT-PCR / BioA only preformed on complete pool.
  |-
  |-
  | 16S Amplicon
  | Sample 2-8 || $235 || $258 || $352
|align="right"| $1,800
|align="right"| $1,980
|align="right"| $2,700
|align="center"|48 samples <BR>(6 columns)
| >5ul, must amplify with Ct <20 by qPCR.
| Includes normalization, enrichment, pooling and quality control (RT-PCR and BioAnalyzer).
  |-
  |-
  | Other Amplicon
  !Oxford Nanopore Sample Preparation
  |align="right"| $1,440
  !width=120| Nanopore Kit
  |align="right"| $1,584
  !width=80| [[BioMicroCenter:CoreDeps|CORE LAB]]
  |align="right"| $2,160
  !width=80| MIT
  |align="center"|48 samples <BR>(6 columns)
  !width=80| [[BioMicroCenter:FAQ#NON_MIT_USERS|Non-MIT]]
| >5ul, must amplify with Ct <20 by qPCR.
  ! Notes
  | Includes normalization, enrichment, pooling and quality control (RT-PCR and BioAnalyzer). <BR> Samples *must* be submitted amplified with appropriate linkers.
  |-
  |-
  | 3'DGE
  |Standard Ligation || LSK || $160 || $176 || $224
|align="right"| $1,200
  |rowspan=6|Oxford Nanopore prep methods remain highly experimental and are continually in flux. <BR> As such, we can only estimate cost based on reagents/effort and cannot guarantee results.<BR> Please consult the core prior to sample submission for the latest information.
|align="right"| $1,320
  |-
  |align="right"| $1,800
|Native Barcoding <br> (ligation indexing) || - || $50 || $55 || $70
  |align="center"|24 samples <BR>(2 rows)
|-
  |5ul at 4 ng/uL of RIN7+ polyA+ RNA.
  |SMARTseq -> Ligation || Cv4+LSK || $300 || $330 || $420
  |Includes cDNA generation, amplification, pooling and quality control (RT-PCR and BioAnalyzer).
|-
  |Rapid Prep (tagment - ~10-20kb) || RAD || $150 || $165 || $210
  |-
  |-
  | colspan="7" | '''ADDITIONAL FEES''' <BR> Samples not in ideal input format will be subject to these additional charges.
  |Long Insert Prep || RAD (modified, target >100kb) || $262 || $289 || $394
  |-
  |-
  |Setup
  |Direct RNA || RNA002 || $160 || $176 || $225
|align="right"| $50
|}
  |align="right"| $55
 
  |align="right"| $75
== SAMPLE EXTRACTION ==
  |align="center"|48 samples
=== CHEMAGIC360 ===
  |  
{| class="wikitable" border=1
  |
!TYPE
  !width=120| Batch Size
  !width=80| [[BioMicroCenter:CoreDeps|CORE LAB]]
  !width=80| MIT
  !width=80| [[BioMicroCenter:FAQ#NON_MIT_USERS|Non-MIT]]
  ! Notes
  |-
  |-
  |Cleanup
  |DNA
  |align="right"| $5
  |96
  |align="right"| $5.5
  |$600
  |align="right"| $7.5
  |$660
  |align="center"|per sample
  |$840
  |
  |
|SPRI cleanup of samples to remove impurities.
  |-
  |-
  |Quantification
  |RNA
  |align="right"| $5
  |96
  |align="right"| $5.5
  |$700
  |align="right"| $7.5
  |$770
  |align="center"|per sample
  |$980
  |
  |
|Quantification of samples (FA) prior to arraying.
|-
|}
|}


== [[BioMicroCenter:MicroArrays|MICROARRAYS]] ==
== SINGLE CELL & SPATIAL SERVICES ==
Microarray processing prices '''do not''' include the cost of the array.  In order to be sure we have arrays on hand, we encourage users to contact us several days in advance of submitting samples.
=== 10X CHROMIUM ===
 
{| class="wikitable" border=1
{| border=1
  ! 10X USAGE
  ! MICROARRAYS
!width=120| Batch Size
  !width=100| [[BioMicroCenter:CoreDeps|CORE LAB]]
  !width=80| [[BioMicroCenter:CoreDeps|CORE LAB]]
  !width=100| MIT
  !width=80| MIT
  !width=100| [[BioMicroCenter:FAQ#NON_MIT_USERS|Non-MIT]]
  !width=80| [[BioMicroCenter:FAQ#NON_MIT_USERS|Non-MIT]]
!width=100| unit
  ! Notes
  ! Notes
  |-
|colspan=6 align="center"|'''Assisted Service'''
|-
|colspan=6 |''DIGITAL GENE EXPRESSION''
|-
|rowspan=2 |5' RNA <br> 3' RNA
|setup || $1,015 || $1,116.50 || $1,421 || charge per submission
|-
|per sample|| $1,750 || $1,925 || $2,450  || Includes QC of cDNA and creation of Short Read Library
|-
|rowspan=2 |Fixed RNA <br> probe based. human/mouse only
|setup || $1300 || $1430 || $1820 || charge per submission
|-
|per sample (4plex) || $3900|| $4290 || $5460 || Includes reagents for hashing of 4 samples in one channel.<br>QC of cDNA and creation of Short Read Library
  |-
  |-
  | colspan="6" | '''AFFYMETRIX PROCESSING'''
  |rowspan=2 |Amplicon (CITE-SEQ, TCR, BCR, antibody barcocde, etc // per amplicon)
|setup || $470 || $517 || $658 || additional prep beyond DGE
|-
|per sample|| $45 || $50 || $63  || Includes QC of cDNA and creation of Short Read Library
  |-
  |-
  | Eukaryote (3' or Exon: >20ng)
  |colspan=6 |''scATACseq''
  |align="right"| $200
  |-
  |align="right"| $250
|rowspan=2 |ATACseq
|align="right"| $325
  |setup || $880 || $968 || $1,232 || charge per submission
  |align="center"| per sample
  |-
| Using AFFYMETRIX sample labeling kit. For NuGEN reagents, please refer to 2015 pricing.
|per sample|| $1,675|| $1,843|| $2,345|| Includes QC and creation of Short Read Library
  |-
  |-
  | Eukaryote (<20ng)
  |colspan=6 |''Multiome (ATAC+3'RNA)''
  |align="right"| $400
  |-
  |align="right"| $500
|rowspan=2 |MULTI
|align="right"| $750
  |setup || $1,870|| $2,057|| $2,618|| charge per submission
  |align="center"| per sample
  |-
| Using NuGEN sample labeling kit.
|per sample|| $3,100|| $3,410|| $4,340|| Includes QC of cDNA and creation of Short Read Library
  |-
  |-
  | Prokaryote
  |colspan=6 |''CANCELLATION''
|align="right"| $365
|align="right"| $455
|align="right"| $683
|align="center"| per sample
| Using Ambion sample labeling kit. 
  |-
  |-
  | colspan="6" | '''AGILENT PROCESSING'''
  |per session || || $200|| $220|| $280|| <24h notice
|}
*please let BMC know if you plan to run this method early so we will have reagents ready for you.
 
=== NAMOCELL ===
{| class="wikitable" border=1
! NAMOCELL
!width=120| Batch Size
!width=80| [[BioMicroCenter:CoreDeps|CORE LAB]]
!width=80| MIT
!width=80| [[BioMicroCenter:FAQ#NON_MIT_USERS|Non-MIT]]
! Notes
|-
|Usage || Per run || $20 || $22 || $30 || per hour
  |-
  |-
  | colspan="6" align=center |Agilent array services are being phased out in FY2016. Requests that must be processed on Agilent will be priced per experiment.
  |Cartridge|| 1 cartridge per sample|| $60 || $66 || $90 ||
|}
 
=== VISIUM ===
{| class="wikitable" border=1
! SLIDES
!width=120| Batch Size
!width=80| KI Only
!width=80| [[BioMicroCenter:CoreDeps|Other CORE LAB]]
!width=80| MIT
!width=80| [[BioMicroCenter:FAQ#NON_MIT_USERS|Non-MIT]]
! Notes
  |-  
  |-  
  | Scanning Only
  |Test Array ||slide|| $1,300|| $1,511|| $1,607|| $2435|| recommended 1x per tissue type for fresh frozen
|align="right"| $50
  |-
  |align="right"| $50
  |Sample Slide - Fresh Frozen ||1 slide / 4 tiles|| $6,210|| $6,340|| $6,940|| $9,067 || Includes H&E stain images + Library Generation
  |align="right"| N/A
  |-
  |align="center"| per slide
  |Sample Slide - FFPE (hs/mm only) ||1 slide / 2 tiles|| $4,550|| $4,550|| $4,996|| $6,359 || Includes library generation only.
  | Price per glass slide.
  |-  
  |-  
|Sample Slide - HD (hs/mm only) ||1 slide / 2 tiles|| $7,850|| $7,850|| $8,596|| $10,940 || Includes library generation only.
|}
|}
prices effective 7.1.2015
 
== OTHER GENOMICS SERVICES ==
 
<br>
<br>


'''Common Affymetrix Arrays* (some may be in stock):'''
=== [[BioMicroCenter:QC|QUALITY CONTROL]] ===
{| class="wikitable" border=1
$175  - Prokaryote/Small Eukaryote                E.coli Genome
  ! QUALITY CONTROL
$250  - Eukaryote 3' (low density)                Human U133A 2.0 / Mouse 430A 2.0
!width=100| unit
$400  - Eukaryote Exon or 3' (high density)      Human U133+ 2.0 / Mouse 430 2.0 / Human or Mouse Exon 1.0 ST
  !width=100| [[BioMicroCenter:CoreDeps|CORE LAB]]
Full Price List:            [[Media:Affy_Arrays_081218.pdf| Affymetrix Prices]]
!width=100| MIT  
* available at cost to academic labs only. shipping fees may be added. Non-academic labs should order their own arrays and have them shipped to the BioMicro Center.
 
== [[BioMicroCenter:2100BioAnalyzer|AGILENT BIOANALYZER]] ==
{| border=1
  ! AGILENT BIOANALYZER / ADVANCED ANALYTICAL-FRAGMENT ANALYZER
  !width=100| [[BioMicroCenter:CoreDeps|CORE LAB]] / MIT
  !width=100| [[BioMicroCenter:FAQ#NON_MIT_USERS|Non-MIT]]
  !width=100| [[BioMicroCenter:FAQ#NON_MIT_USERS|Non-MIT]]
!width=100| unit
  ! Notes
  ! Notes
  |-
  |-
  | RNA / DNA on Advanced Analytical
  |Fragment Analyzer: RNA or DNA
  |align="right"| $8
  |per sample ||$10 || $11 || $14
  |align="right"| $12
  |
  |align="center"| per sample
|-
|Fragment Analyzer: Preloaded row
| per row (12) ||$50 ||$55 || $70
| 2ul per sample in each well using plates from BMC
|-
  |FemtoPulse: RNA or DNA
|per sample || $14 || $15.4 || $19.6
  |  
  |  
  |-
  |-
  | AA - Preloaded plate
  |FemtoPulse: Preloaded Row
  |align="right"| $30
  |per row (12) || $70 || $77 || $98
|align="right"| N/A
|align="center"| per row (12)
  | 2ul per sample in each well using plates from BMC
  | 2ul per sample in each well using plates from BMC
  |-
  |-
  | All DNA & RNA on BIOANALYZER
  |BioAnalyzer: Assisted Service (RNA or DNA)
  |align="right"| $15
  |per sample || $15 || $18 || $25
|align="right"| $25
|align="center"| per sample
  |  
  |  
  |-
  |-
  | High Sensitivity protein
  |BioAnalyzer: Assisted Service (High Sensitivity protein)
  |align="right"| $40
  |per sample || $40 || $44 || $60
|align="right"| $60
|align="center"| per sample
  | detection of proteins at < 30ng/ul.   
  | detection of proteins at < 30ng/ul.   
|-
|qPCR only (Illumina quantification test)
|per sample || $18 || $19.8 || $25.2
| 4 point serial dilution
|-
|Full plate qPCR (Illumina quantification test)
|per plate || $330 || $363 || $462
| full plate only (exclude column 1 for standards) - must be to BMC specifications.
  |-
  |-
|}
|}
prices effective 7.1.2015
 
<br>.
=== OLIGO SYNTHESIS ===
{| class="wikitable" border=1
!Platform
!width=100| Service
!width=100| [[BioMicroCenter:CoreDeps|CORE LAB]]
!width=100| MIT
!width=100| [[BioMicroCenter:FAQ#NON_MIT_USERS|Non-MIT]]
! Notes
|-
|rowspan=3| Dr.Oligo
|Assisted
|$1000<br>+consumables
|$1100<br>+consumables
|$1400<br>+consumables
|includes setup and run of the instrument only. Large reagents / N2 included.
|-
|Walkup
|$500<br>+consumables
|$550<br>+consumables
|NA
|includes run of the instrument only. Large reagents / N2 included.
|-
|Training
|$2000
|$2200
|NA
|ncludes two instrument runs.
|-
|rowspan=6| DNAscript Syntax
|Setup (per run)
|$1000
|$1100
|$1400
|Per run cost
|-
|2000 nucleotide synthesis
|$500
|$550
|$700
|Up to 96 samples
|-
|8000 nucleotide synthesis
|$1500
|$1650
|$2100
|Up to 96 samples
|-
|add Biotin (max48)
|$115
|$126
|$161
|rowspan = 3| max 48 per run and 2 per oligo. Couple to T only.
|-
|add Fluor
|$300
|$330
|$420
|-
|add quench
|$100
|$110
|$140
|}


== [[BioMicroCenter:RTPCR|REAL TIME PCR]] ==
== [[BioMicroCenter:RTPCR|REAL TIME PCR]] ==
Line 436: Line 871:
  |align="right"| N/A
  |align="right"| N/A
  |align="center"| per plate
  |align="center"| per plate
  | [[BioMicroCenter:Forms#Calenders|CALENDER]] *available for CORE only
  | [[BioMicroCenter:Forms#Calenders|CALENDAR]] *available for CORE only
  | Contact [[BioMicroCenter:People|Jon Penterman]] for training
  | Contact [[BioMicroCenter:People|Christopher Hallee]] for training
  |-
  |-
  | SYBR Green (KAPA/Roche)
  | SYBR Green (KAPA/Roche)
Line 458: Line 893:


== OTHER LAB SERVICES ==
== OTHER LAB SERVICES ==
{| border=1
{| border=1
  ! OTHER LAB SERVICES
  ! OTHER LAB SERVICES
Line 466: Line 900:
  ! SIGN UP
  ! SIGN UP
  ! Notes
  ! Notes
|-
|colspan=6 align="center"| '''COVARIS SONICATOR'''
|-
|COVARIS E220
|align="right"|$20
|align="right"|$30
|per hour
|[https://mit.ilabsolutions.com/equipment/261752/?tab=schedule ilab calendar]
|tubes available for purchase.
  |-
  |-
  | colspan="6" align="center"| '''PIPPIN PREP'''
  | colspan="6" align="center"| '''PIPPIN PREP'''
  |-
  |-
  |Gel Isolation
  |Gel Isolation
|align="right"| $50
  |align="right"| $75
  |align="right"| $75
|align="right"| $105
  |align="center"| per gel (5 lanes)
  |align="center"| per gel (5 lanes)
  | TBA
  | TBA
Line 481: Line 925:
  |align="center"| per session
  |align="center"| per session
  | TBA
  | TBA
  | Contact [[BioMicroCenter:People|Jon Penterman]] for training.
  | Contact [[BioMicroCenter:People|Noelani Kamelamela]] for training.
  |-
  |-
| colspan="6" align="center"| '''PLATE READER'''
| colspan="6" align="center"| '''PLATE READER'''
Line 489: Line 933:
  |align="right"| $27
  |align="right"| $27
  |align="center"| per hour
  |align="center"| per hour
  | [[BioMicroCenter:Forms#Calenders|CALENDER]]
  | [[BioMicroCenter:Forms#Calenders|CALENDAR]]
  | Contact [[BioMicroCenter:People|Jon Penterman]] for training  
  | Contact [[BioMicroCenter:People|Noelani Kamelamela]] for training  
  |-
  |-
  | colspan="6" align="center"| '''ROBOTICS'''
  | colspan="6" align="center"| '''ROBOTICS'''
Line 498: Line 942:
  |align="right"| $55
  |align="right"| $55
  |align="center"| per 1h block - based on scheduled time.
  |align="center"| per 1h block - based on scheduled time.
  | [[BioMicroCenter:Forms#Calenders|CALENDER]]
  | [[BioMicroCenter:Forms#Calenders|CALENDAR]]
  |  
  |  
  |-
  |-
  |non-filter tips
  |non-filter tips
  |align="right"| $6.00
  |align="right"| $7.00
  |align="right"| $6.00
  |align="right"| $7.70
  |align="center"| per box
  |align="center"| per box
  |
  |
Line 509: Line 953:
  |-
  |-
  |Filter tips
  |Filter tips
  |align="right"| $8.00
  |align="right"| $10.00
  |align="right"| $8.00
  |align="right"| $11.00
  |align="center"| per box
  |align="center"| per box
  |
  |
Line 516: Line 960:
  |-
  |-
  | Training
  | Training
  |align="right"| $50
  |align="right"| $65
  |align="right"| $55
  |align="right"| $71.5
  |align="center"| per session
  |align="center"| per hour
  | Contact [[BioMicroCenter:People|Stuart]]
  | Contact [[BioMicroCenter:People|Tatum Murdock]]
  |  
  |  
  |-
  |-
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== COMPUTATIONAL SERVICES ==   
== COMPUTATIONAL SERVICES ==   
{| border=1
{| class="wikitable" border=1
  ! COMPUTATIONAL SERVICES
  ! COMPUTATIONAL SERVICES
!width=200| unit
  !width=100| [[BioMicroCenter:CoreDeps|CORE LAB]]
  !width=100| [[BioMicroCenter:CoreDeps|CORE LAB]]
  !width=100| Other MIT / [[BioMicroCenter:FAQ#NON_MIT_USERS|Non-MIT]]
  !width=100| Other MIT / non-MIT  
!width=200| unit
  ! Notes
  ! Notes
|-
  |-
  | Informatics Support
  | Informatics Support
| align="right"| $75
| align="right"| $100 / $130
  | align="right"| per hour
  | align="right"| per hour
  | Limited support for non-MIT users
  | align="right"| $90
| align="right"| $125 / $180
| Very limited support for non-MIT users
  |-
  |-
  | Informatics Share
  | Informatics Share
  | align="right"| $1,000
| align="right"| per 0.1FTE
  | align="right"| $1,250
  | align="right"| N/A
  | align="right"| N/A
| align="right"| per 0.1FTE
  | MIT only. Priority access to informatics.
  | MIT only. Priority access to informatics.
  |-
  |-
  | Data storage (1 year)
  |Ingenuity Pathway Analysis
  |align="right"| $280
  |align="right"|per dataset
  |align="right"| $400 / N/A
  |align="right" | $100
  |align="center"| per TB
  |align="right" | $110/$150
  |  
  | Recovery of licence cost.
  |-
  |-
  | Archival Storage (1 year - read only. Unmutable)
  |Active Data storage
  |align="right"| $140
  |align="right"| per TB per year
  |align="right"| $200 / N/A
  |align="right"| $100
  |align="center"| per TB
  |align="right"| $300 / N/A
  |  
  |
  |-
  |-
  | Tape Archive / Recovery
  | Tape Archive / Recovery
|align="right"| per tape
  |align="right"| $60
  |align="right"| $60
  |align="right"| $70
  |align="right"| $70
|align="center"| per tape
  |  
  |  
  |-
  |-
| Informatics Training Classes
|align="right"| per session
|align="right"| $50
|align="right"| $100
|
|}
|}


OLD<BR>
[[BioMicroCenter:PricingFY2023b | FY2023-Feb Pricing]]<BR>
[[BioMicroCenter:PricingFY2023 | FY2023 Pricing]]<BR>
[[BioMicroCenter:PricingFY2022 | FY2022 Pricing]]<BR>
[[BioMicroCenter:PricingFY2021 | FY2021 Pricing]]<BR>
[[BioMicroCenter:PricingFY2019 | FY2019 Pricing]]<BR>
[[BioMicroCenter:PricingFY2017 | FY2017 Pricing]]<BR>
[[BioMicroCenter:PricingFY2017 | FY2017 Pricing]]<BR>
[[BioMicroCenter:PricingFY2016 | FY2016 Pricing]]<BR>
[[BioMicroCenter:PricingFY2016 | FY2016 Pricing]]<BR>

Latest revision as of 13:03, 18 April 2024

HOME -- SEQUENCING -- LIBRARY PREP -- HIGH-THROUGHPUT -- COMPUTING -- OTHER TECHNOLOGY

PRICING UPDATE 2/1/2023

SEQUENCING

Please note that samples submitted from CORE LABs will be given priority on all equipment. The BioMicro Center reserves the right to reject samples for any reason.

NOVASEQ SEQUENCING

NOVASEQ SEQUENCING CORE LAB MIT Non-MIT unit Notes
NovaSeq SP 100nt $3,699 $4,068 $5,178 Per flowcell Includes final pool quality control (RT-PCR and BioAnalyzer), sequencing, genome alignment and data storage of FASTQ files for 1 year
NovaSeq SP 300nt** $5,238 $5,761 $7,333.20
NovaSeq SP 500nt $7,020 $7,722 $9,828
NovaSeq S1 100nt** $5,832 $6,415.20 $8,164.80
NovaSeq S1 200nt** $7,452 $8,197.20 $10,432
NovaSeq S1 300nt** $8,127 $8,939.70 $11,377.80
NovaSeq S2 100nt** $10,395 $11,434.50 $14,553
NovaSeq S2 200nt** $12,960 $14,256 $18,144
NovaSeq S2 300nt** $13,500 $14,850 $18,900
NovaSeq S4 50nt $3,780 $4,158 $5,292 Per Lane Includes final pool quality control (RT-PCR and BioAnalyzer), sequencing, genome alignment and data storage of FASTQ files for 1 year
NovaSeq S4 300nt $5,400 $5,940 $7,560 
** - minimal inventory maintained

NEXTSEQ500 SEQUENCING

NEXTSEQ SEQUENCING CORE LAB MIT Non-MIT unit Notes
91nt kit
(eg. 40PE or 75SE) 		$2,420 $2,662 $3,388 per flowcell Includes final pool quality control (RT-PCR and BioAnalyzer), sequencing, genome alignment and data storage of FASTQ files for 1 year
166nt kit
(eg. 80PE or 150SE) 		$4,125 $4,537.50 $5,775
316nt kit
(eg. 150PE or 300SE) 		$6,270 $6,870 $8,778

MISEQ SEQUENCING

MISEQ SEQUENCING CORE LAB MIT Non-MIT unit Notes
70nt V2 kit
(eg. 30PE or 70SE) 		$1,320 $1,452 $1,848 per lane Includes final pool quality control (RT-PCR and BioAnalyzer), sequencing, genome alignment and data storage of FASTQ files for 1 year
150nt V3 kits
(eg. 75PE) 		$1,430 $1,573 $2,002
300nt V2 kit
(eg. 150PE) 		$1,595 $1,754.50 $2,233
500nt V2 kit
(eg. 250PE) 		$1,760 $1,936 $2,464
600nt V3 kit
(eg. 300PE) 		$2,200 $2,420 $3,080
500nt NANO Run
(eg. 250PE) 		$770 $847 $1,078

SINGULAR G4

SINGULAR G4 SEQUENCING CORE LAB MIT Non-MIT unit Notes
50 PE: F3 Lane (~100m reads) $300 $330 $420 per lane Includes final pool quality control (RT-PCR and FA), sequencing, and data storage of FASTQ files for 1 year
150 PE: F3 Lane (~100m reads) $450 $495 $630
Rapid 300nt: F3 flowcell (~400m reads)* $1800 $1980 $2520 per flowcell 
* Rapid flowcells are required for adapted Illumina libraries.

ELEMENT AVITI

ELEMENT AVITI SEQUENCING Lane/Flowcell CORE LAB MIT Non-MIT unit Notes
75 PE Full flowcell (2 lane, 800m read) $1,900 $2,090 $2,660 per flowcell Includes final pool quality control (RT-PCR and FA), sequencing, and data storage of FASTQ files for 1 year
One lane (~400m read) $1,000 $1,100 $1,400 per lane
150 PE Full flowcell (2 lane, ~800m read) $2,650 $2,915 $3,710 per flowcell
One lane (~400m read) $1,375 $1,513 $1,925 per lane
300 PE Full flowcell (~200m reads) $3,500 $3,850 $4,900 per lane

Pricing effective 12/1/2023

OTHER SHORT READ SERVICES

ILLUMINA SUPPORTING SERVICES CORE LAB MIT Non-MIT unit Notes
Quality Control Only -only one QC is included per sequencing lane. $25 $27.50 $35 per sample Includes RT-PCR and Frag.Analyzer
Rapid Quality Control - Use SYBR for sample quantification for pooling instead of qPCR. Only available for pooling. $12 $13.20 $16.80 per sample Includes Fluorometric quantification and Frag.Analyzer
qPCR only (short read) $18 $19.80 $25.20 per sample Includes qPCR values from standard QC only
qPCR only - full plate(short read) $330 $363 $462 per sample excludes column 1 (used for controls)

LONG READ SEQUENCING

SEQUEL SEQUENCING CORE LAB MIT Non-MIT unit Notes
20 hour v3 Chemistry $1,470 $1,617 $2,205 per SMRTCell Includes polymerase binding and movie.
PROMETHION SEQUENCING CORE LAB MIT Non-MIT unit Notes
72 hour Flowcell $1,200 $1,320 $1,680 per Flowcell Fastq stored for 1y. Trace data stored only 30d.

LIBRARY GENERATION

SHORT READ LIBRARY - CONVERSION

Input library Convert To Method CORE LAB MIT Non-MIT Notes
Illumina Singular Index Overwrite (per sample) $30 $33 $42 Includes QC and reindexing
Illumina Singular P5/P7 conversion (per pool) $100 $110 $140 Includes QC. Samples not reindexed
Requires full flowcell for sequencing (Rapid)
Illumina Element Index Overwrite (per sample) $30 $33 $42 Includes QC and reindexing - "Elevate" workflow
Illumina Element P5/P7 splicing (per pool) $100 $110 $140 Includes QC. Samples not reindexed. Requires high input amounts (>10nM)

SHORT READ LIBRARY - DNA

ILLUMINA Sample Preparation Type Batch Size CORE LAB MIT Non-MIT Notes
STANDARD THROUGHPUT DNA
Prices for individual samples. Repreps will typically be attempted on failed samples without charge. 		HIGH THROUGHPUT DNA
Prices for sample batches. Repreps will NOT be attempted on failed samples without charge. Low volume (<=5ul) submission must be coordinated with BMC staff. Initial QC not included.
LIGATION BASED
NEB UltraII 		STD per sample $105 $115 $157 Includes QC, adapter ligation, size selection, barcoding, enrichment, and quality control (SYBR and FA).
HTL 24 $825 $907.50 $1,155 Includes adapter ligation, size selection, barcoding, enrichment, and quality control spotcheck (SYBR and FA).
96 $1,625 $1,787.50 $2,275
Tagmentation
NEXTERA-XT 		STD per Sample $105 $115 $157 Includes set-up, tagmentation, indexing, enrichment, and quality control (SYBR and FA).
Tagmentation
NEXTERA-FLEX 		$157 $173 $236
NEXTERA-XT OR FLEX HTL 24 $825 $907.50 $1,155 Includes tagmentation, indexing, enrichment, and quality control spotcheck(SYBR and FA).
96 $1,625 $1,787.50 $2,275
16S Amplicon HTL 48 $1,900 $2,090 $2,660 Includes normalization, enrichment, pooling and quality control (SYBR and FA).
add'l 48 $650 $715 $910
Other Amplicon HTL 48 $1,600 $1,760 $2,240 Includes normalization, enrichment, pooling and quality control (SYBR and FA).
Samples *must* be submitted amplified with appropriate linkers.
add'l 48 $630 $693 $945
ADDITIONAL FEES
Samples not in proper input format will be subject to these additional charges.
High Throughput Setup 48 samples $50 $55 $75
Sample Cleanup per sample $5 $5.50 $7 SPRI cleanup of samples to remove impurities.
Quantification per sample $6 $6.60 $8.40 Quantification of samples (FA) prior to arraying.

SHORT READ LIBRARY - RNA

ILLUMINA Sample Preparation Type Batch Size CORE LAB MIT Non-MIT Notes
STANDARD RNA
Prices for individual samples. Repreps will typically be attempted on failed samples without charge. 		HIGH THROUGHPUT RNA
Repreps will NOT be attempted on failed samples without charge. Low volume (<=5ul) submission must be coordinated with BMC staff. Initial QC not included
polyA based isolation

NEB UltraII RNAseq 		STD per sample $157 $173 $236 Includes QC, polyA isolation, generation of cDNA, library construction, indexing and quality control (SYBR + FA).
HTL
(NEB only) 		24 $1,400 $1,540 $1,960 Includes polyA isolation, generation of cDNA, library construction, indexing and spot QC (SYBR + FA).
96 $2,800 $3,080 $3,920 Includes polyA isolation, generation of cDNA, library construction, indexing and spot QC (SYBR + FA).
High Throughput 3' Digital Gene Expression (HT3DGE) HTL 24 $1,050 $1,155 $1,470 Includes generation of cDNA, library construction, indexing and library quality control. Does not include sample QC.
96 $2,100 $2,310 $2,940
Total RNA sequencing

NEB RNAseq +Ribodepletion 		STD per sample $235 $258.5 $352.5 Includes sample QC, ribosomal depletion, generation of cDNA, library construction, indexing and library quality control.
HTL
(NEB only, covaris RNA fragmentation) 		24 $2,400 $2,640 $3,360 Includes polyA isolation, generation of cDNA, library construction, indexing and spot QC (SYBR + FA).
96 $4,800 $5,280 $6,720
Low Input polyA+
Clontech SMARTseq + NexteraXT or Flex 		STD
v4 		per sample $262 $289 $394 Includes generation of cDNA, amplification, library construction, indexing and quality control (SYBR + FA). Single sample includes initial QC.
HTL
v2 		24 $1880 $1980 $2520
96 $2800 $3080 $3920
Low Input Mammalian Total
Clontech ZapR Kit 		STD per sample $210 $231 $315 Includes generation of cDNA, amplification, library construction, rRNA degradation, indexing and quality control (SYBR + FA).
HTL
(covaris RNA fragmentation) 		24 $2200 $2420 $3080 Includes polyA isolation, generation of cDNA, library construction, indexing and spot QC (SYBR + FA).
96 $4400 $4840 $6160
Small RNA STD per sample $289 $318 $433 Uses NEB, BIOO or QIAGEN small RNA kit
ADDITIONAL FEES
Samples not in proper input format will be subject to these additional charges.
High Throughput Setup 48 samples $50 $55 $75
Sample Cleanup per sample $5 $5.50 $7.50 SPRI cleanup of samples to remove impurities.
Quantification per sample $6 $6.60 $8.40 Quantification of samples (FA) prior to arraying.

.

LONG READ LIBRARIES

PacBio Sample Preparation Batch Size CORE LAB MIT Non-MIT Notes
SMRTBell Only
eg:PCR products 		Sample 1 $315 $346 $472 Includes QC, repair and addition of indexed SMRTBells
Samples 2-8 $157 $173 $236
Genomic Library Sample 1 $472 $520 $709 Includes Covaris gSPIN fragmentation, Pippin sizing, repair and SMRTbell as above
Sample 2-8 $235 $258 $352
Oxford Nanopore Sample Preparation Nanopore Kit CORE LAB MIT Non-MIT Notes
Standard Ligation LSK $160 $176 $224 Oxford Nanopore prep methods remain highly experimental and are continually in flux.
As such, we can only estimate cost based on reagents/effort and cannot guarantee results.
Please consult the core prior to sample submission for the latest information.
Native Barcoding
(ligation indexing)		 - $50 $55 $70
SMARTseq -> Ligation Cv4+LSK $300 $330 $420
Rapid Prep (tagment - ~10-20kb) RAD $150 $165 $210
Long Insert Prep RAD (modified, target >100kb) $262 $289 $394
Direct RNA RNA002 $160 $176 $225

SAMPLE EXTRACTION

CHEMAGIC360

TYPE Batch Size CORE LAB MIT Non-MIT Notes
DNA 96 $600 $660 $840
RNA 96 $700 $770 $980

SINGLE CELL & SPATIAL SERVICES

10X CHROMIUM

10X USAGE Batch Size CORE LAB MIT Non-MIT Notes
Assisted Service
DIGITAL GENE EXPRESSION
5' RNA
3' RNA 		setup $1,015 $1,116.50 $1,421 charge per submission
per sample $1,750 $1,925 $2,450 Includes QC of cDNA and creation of Short Read Library
Fixed RNA
probe based. human/mouse only 		setup $1300 $1430 $1820 charge per submission
per sample (4plex) $3900 $4290 $5460 Includes reagents for hashing of 4 samples in one channel.
QC of cDNA and creation of Short Read Library
Amplicon (CITE-SEQ, TCR, BCR, antibody barcocde, etc // per amplicon) setup $470 $517 $658 additional prep beyond DGE
per sample $45 $50 $63 Includes QC of cDNA and creation of Short Read Library
scATACseq
ATACseq setup $880 $968 $1,232 charge per submission
per sample $1,675 $1,843 $2,345 Includes QC and creation of Short Read Library
Multiome (ATAC+3'RNA)
MULTI setup $1,870 $2,057 $2,618 charge per submission
per sample $3,100 $3,410 $4,340 Includes QC of cDNA and creation of Short Read Library
CANCELLATION
per session $200 $220 $280 <24h notice
  • please let BMC know if you plan to run this method early so we will have reagents ready for you.

NAMOCELL

NAMOCELL Batch Size CORE LAB MIT Non-MIT Notes
Usage Per run $20 $22 $30 per hour
Cartridge 1 cartridge per sample $60 $66 $90

VISIUM

SLIDES Batch Size KI Only Other CORE LAB MIT Non-MIT Notes
Test Array slide $1,300 $1,511 $1,607 $2435 recommended 1x per tissue type for fresh frozen
Sample Slide - Fresh Frozen 1 slide / 4 tiles $6,210 $6,340 $6,940 $9,067 Includes H&E stain images + Library Generation
Sample Slide - FFPE (hs/mm only) 1 slide / 2 tiles $4,550 $4,550 $4,996 $6,359 Includes library generation only.
Sample Slide - HD (hs/mm only) 1 slide / 2 tiles $7,850 $7,850 $8,596 $10,940 Includes library generation only.

OTHER GENOMICS SERVICES


QUALITY CONTROL

QUALITY CONTROL unit CORE LAB MIT Non-MIT Notes
Fragment Analyzer: RNA or DNA per sample $10 $11 $14
Fragment Analyzer: Preloaded row per row (12) $50 $55 $70 2ul per sample in each well using plates from BMC
FemtoPulse: RNA or DNA per sample $14 $15.4 $19.6
FemtoPulse: Preloaded Row per row (12) $70 $77 $98 2ul per sample in each well using plates from BMC
BioAnalyzer: Assisted Service (RNA or DNA) per sample $15 $18 $25
BioAnalyzer: Assisted Service (High Sensitivity protein) per sample $40 $44 $60 detection of proteins at < 30ng/ul.
qPCR only (Illumina quantification test) per sample $18 $19.8 $25.2 4 point serial dilution
Full plate qPCR (Illumina quantification test) per plate $330 $363 $462 full plate only (exclude column 1 for standards) - must be to BMC specifications.

OLIGO SYNTHESIS

Platform Service CORE LAB MIT Non-MIT Notes
Dr.Oligo Assisted $1000
+consumables 		$1100
+consumables 		$1400
+consumables 		includes setup and run of the instrument only. Large reagents / N2 included.
Walkup $500
+consumables 		$550
+consumables 		NA includes run of the instrument only. Large reagents / N2 included.
Training $2000 $2200 NA ncludes two instrument runs.
DNAscript Syntax Setup (per run) $1000 $1100 $1400 Per run cost
2000 nucleotide synthesis $500 $550 $700 Up to 96 samples
8000 nucleotide synthesis $1500 $1650 $2100 Up to 96 samples
add Biotin (max48) $115 $126 $161 max 48 per run and 2 per oligo. Couple to T only.
add Fluor $300 $330 $420
add quench $100 $110 $140

REAL TIME PCR

OTHER LAB SERVICES CORE LAB / MIT Non-MIT unit SIGN UP Notes
Roche LightCycler 480 $10 N/A per plate CALENDAR *available for CORE only Contact Christopher Hallee for training
SYBR Green (KAPA/Roche) $36 N/A per ml Available for MIT only
96-well PLATES $6 N/A per plate Available for MIT only

.

OTHER LAB SERVICES

OTHER LAB SERVICES CORE LAB / MIT Non-MIT unit SIGN UP Notes
COVARIS SONICATOR
COVARIS E220 $20 $30 per hour ilab calendar tubes available for purchase.
PIPPIN PREP
Gel Isolation $75 $105 per gel (5 lanes) TBA
Training $25 $50 per session TBA Contact Noelani Kamelamela for training.
PLATE READER
Varioskan $18 $27 per hour CALENDAR Contact Noelani Kamelamela for training
ROBOTICS
Tecan EVO 150 $50 $55 per 1h block - based on scheduled time. CALENDAR
non-filter tips $7.00 $7.70 per box ** Please let us know if there is additional plasticware you would like us to stock.
Filter tips $10.00 $11.00 per box
Training $65 $71.5 per hour Contact Tatum Murdock
NANODROP
Nanodrop -- -- Walk up service * no charge



COMPUTATIONAL SERVICES

COMPUTATIONAL SERVICES unit CORE LAB Other MIT / non-MIT Notes
Informatics Support per hour $90 $125 / $180 Very limited support for non-MIT users
Informatics Share per 0.1FTE $1,250 N/A MIT only. Priority access to informatics.
Ingenuity Pathway Analysis per dataset $100 $110/$150 Recovery of licence cost.
Active Data storage per TB per year $100 $300 / N/A
Tape Archive / Recovery per tape $60 $70
Informatics Training Classes per session $50 $100


OLD
FY2023-Feb Pricing
FY2023 Pricing
FY2022 Pricing
FY2021 Pricing
FY2019 Pricing
FY2017 Pricing
FY2016 Pricing
FY2015 Pricing
FY2014 Pricing
FY2013 Pricing
FY2012 Pricing
FY2011 Pricing

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