BioMicroCenter:Pricing: Difference between revisions
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{{BioMicroCenter}} | {{BioMicroCenter}} | ||
== SEQUENCING == | |||
Please note that samples submitted from [[BioMicroCenter:CoreDeps|'''CORE LAB''']]s will be given priority on the HiSeq. | Please note that samples submitted from [[BioMicroCenter:CoreDeps|'''CORE LAB''']]s will be given priority on the HiSeq. | ||
Line 14: | Line 14: | ||
|- | |- | ||
| Single End (40nt) | | Single End (40nt) | ||
|align="right"| $1, | |align="right"| $1,140 | ||
|align="right"| $1, | |align="right"| $1,254 | ||
|align="right"| $1, | |align="right"| $1,710 | ||
|align="center"| per lane | |align="center"| per lane | ||
| Includes final pool quality control (RT-PCR and BioAnalyzer), sequencing, genome alignment and data storage of BAM files for 1y. | | Includes final pool quality control (RT-PCR and BioAnalyzer), sequencing, genome alignment and data storage of BAM files for 1y. | ||
|- | |- | ||
| Paired End (40+40nt) | | Paired End (40+40nt) | ||
|align="right"| $1, | |align="right"| $1,710 | ||
|align="right"| $1, | |align="right"| $1,881 | ||
|align="right"| $2, | |align="right"| $2,565 | ||
|align="center"| per lane | |align="center"| per lane | ||
| | | | ||
|- | |- | ||
| Add'l 40nt | | Add'l 40nt | ||
|align="right"| $330 | |align="right"| $330 | ||
|align="right"| $ | |align="right"| $363 | ||
|align="right"| $495 | |||
|align="center"| per lane | |align="center"| per lane | ||
| | | | ||
Line 37: | Line 37: | ||
|- | |- | ||
| 91nt kit <BR> (eg. 40PE or 50SE) | | 91nt kit <BR> (eg. 40PE or 50SE) | ||
|align="right"| $2, | |align="right"| $2,100 | ||
|align="right"| $2, | |align="right"| $2,310 | ||
|align="right"| $3, | |align="right"| $3,150 | ||
|align="center"| per flowcell | |align="center"| per flowcell | ||
| Includes final pool quality control (RT-PCR and BioAnalyzer), sequencing, genome alignment and data storage of BAM files for 1 year <BR> ** THESE RUNS WILL HAVE LOW PRIORITY | | Includes final pool quality control (RT-PCR and BioAnalyzer), sequencing, genome alignment and data storage of BAM files for 1 year <BR> ** THESE RUNS WILL HAVE LOW PRIORITY | ||
|- | |- | ||
| 166nt kit <BR> (eg. 80PE or 150SE) | | 166nt kit <BR> (eg. 80PE or 150SE) | ||
|align="right"| $3, | |align="right"| $3,150 | ||
|align="right"| $3, | |align="right"| $3,465 | ||
|align="right"| $4, | |align="right"| $4,725 | ||
|align="center"| per flowcell | |align="center"| per flowcell | ||
| Includes final pool quality control (RT-PCR and BioAnalyzer), sequencing, genome alignment and data storage of BAM files for 1 year | | Includes final pool quality control (RT-PCR and BioAnalyzer), sequencing, genome alignment and data storage of BAM files for 1 year | ||
|- | |- | ||
| 316nt kit <BR> (eg. 150PE or 300SE) | | 316nt kit <BR> (eg. 150PE or 300SE) | ||
|align="right"| $4, | |align="right"| $4,725 | ||
|align="right"| $ | |align="right"| $5,200 | ||
|align="right"| $ | |align="right"| $7,088 | ||
|align="center"| per flowcell | |align="center"| per flowcell | ||
| Includes final pool quality control (RT-PCR and BioAnalyzer), sequencing, genome alignment and data storage of BAM files for 1 year | | Includes final pool quality control (RT-PCR and BioAnalyzer), sequencing, genome alignment and data storage of BAM files for 1 year | ||
Line 60: | Line 60: | ||
|- | |- | ||
| 70nt Run <BR> (eg. 30PE or 70SE) | | 70nt Run <BR> (eg. 30PE or 70SE) | ||
|align="right"| $1, | |align="right"| $1,155 | ||
|align="right"| $1, | |align="right"| $1,270 | ||
|align="right"| $1, | |align="right"| $1,732 | ||
|align="center"| per lane | |align="center"| per lane | ||
| Includes final pool quality control (RT-PCR and BioAnalyzer), sequencing, genome alignment and data storage of BAM files for 1 year | | Includes final pool quality control (RT-PCR and BioAnalyzer), sequencing, genome alignment and data storage of BAM files for 1 year | ||
|- | |- | ||
| 300nt Run <BR> (eg. 150PE) | | 300nt Run <BR> (eg. 150PE) | ||
|align="right"| $1, | |align="right"| $1,365 | ||
|align="right"| $1, | |align="right"| $1,500 | ||
|align="right"| $ | |align="right"| $2,050 | ||
|align="center"| per lane | |align="center"| per lane | ||
| Includes final pool quality control (RT-PCR and BioAnalyzer), sequencing, genome alignment and data storage of BAM files for 1 year | | Includes final pool quality control (RT-PCR and BioAnalyzer), sequencing, genome alignment and data storage of BAM files for 1 year | ||
|- | |- | ||
| 500nt Run <BR> (eg. 250PE) | | 500nt Run <BR> (eg. 250PE) | ||
|align="right"| $1, | |align="right"| $1,575 | ||
|align="right"| $1, | |align="right"| $1,732 | ||
|align="right"| $2, | |align="right"| $2,362 | ||
|align="center"| per lane | |align="center"| per lane | ||
| Includes final pool quality control (RT-PCR and BioAnalyzer), sequencing, genome alignment and data storage of BAM files for 1 year | | Includes final pool quality control (RT-PCR and BioAnalyzer), sequencing, genome alignment and data storage of BAM files for 1 year | ||
Line 83: | Line 83: | ||
|- | |- | ||
| 150nt Run <BR> (eg. 75PE) | | 150nt Run <BR> (eg. 75PE) | ||
|align="right"| $1, | |align="right"| $1,365 | ||
|align="right"| $1, | |align="right"| $1,500 | ||
|align="right"| $ | |align="right"| $2,050 | ||
|align="center"| per lane | |align="center"| per lane | ||
| Includes final pool quality control (RT-PCR and BioAnalyzer), sequencing, genome alignment and data storage of BAM files for 1 year | | Includes final pool quality control (RT-PCR and BioAnalyzer), sequencing, genome alignment and data storage of BAM files for 1 year | ||
|- | |- | ||
| | | 600nt Run <BR> (eg. 300PE) | ||
| | |align="right"| $1,890 | ||
| | |align="right"| $2,080 | ||
|align="right"| $2,835 | |||
|align="center"| per lane | |||
| Includes final pool quality control (RT-PCR and BioAnalyzer), sequencing, genome alignment and data storage of BAM files for 1 year | |||
|- | |- | ||
| colspan="6" align=center |<BR>'''Additional Services''' | | colspan="6" align=center |<BR>'''Additional Services''' | ||
|- | |- | ||
| colspan="6" align=center |''These services apply to samples submitted for all platforms.'' | | colspan="6" align=center |''These services apply to samples submitted for all platforms.'' | ||
|- | |- | ||
| Quality Control Only -only one QC is included per sequencing lane. | | Quality Control Only -only one QC is included per sequencing lane. | ||
Line 116: | Line 105: | ||
|align="right"| $30 | |align="right"| $30 | ||
|align="center"| per sample | |align="center"| per sample | ||
| Includes RT-PCR and | | Includes RT-PCR and Frag.Analyzer | ||
|- | |||
| Rapid Quality Control - Use SYBR for sample quantification for pooling instead of qPCR. Only available for pooling. | |||
|align="right"| $10 | |||
|align="right"| $13 | |||
|align="right"| $15 | |||
|align="center"| per sample | |||
| Includes Fluorometric quantification and Frag.Analyzer | |||
|} | |} | ||
Line 122: | Line 118: | ||
Please note that samples submitted from [[BioMicroCenter:CoreDeps|'''CORE LAB''']]s will be given priority on all equipment. The BioMicro Center reserves the right to reject samples for any reason.''<BR> | Please note that samples submitted from [[BioMicroCenter:CoreDeps|'''CORE LAB''']]s will be given priority on all equipment. The BioMicro Center reserves the right to reject samples for any reason.''<BR> | ||
=== SAMPLE PREPARATION === | == LIBRARY GENERATION == | ||
=== SAMPLE PREPARATION - DNA === | |||
{| border=1 | {| border=1 | ||
! ILLUMINA Sample Preparation | ! ILLUMINA Sample Preparation | ||
!width=80| [[BioMicroCenter:CoreDeps|CORE LAB]] | !width=80| [[BioMicroCenter:CoreDeps|CORE LAB]] | ||
!width=80| MIT | !width=80| MIT | ||
!width=80| [[BioMicroCenter:FAQ#NON_MIT_USERS|Non-MIT]] | !width=80| [[BioMicroCenter:FAQ#NON_MIT_USERS|Non-MIT]] | ||
!width= | !width=160| unit | ||
!width=160| INPUT REQUIREMENT | |||
! Notes | ! Notes | ||
|- | |- | ||
| colspan=" | | colspan="4" | '''STANDARD THROUGHPUT DNA''' <BR> Prices for individual samples. Repreps will typically be attempted on failed samples without charge. | ||
| colspan="3" style="color: blue;" | '''HIGH THROUGHPUT DNA''' <BR> Prices for individual samples. Repreps will NOT be attempted on failed samples without charge. Low volume (<=5ul) submission must be coordinated with BMC staff. | |||
|- | |||
| NEOPREP DNA <BR> * Neoprep will be retired 7/17 | |||
|align="right"| $70 | |||
|align="right"| $77 | |||
|align="right"| $105 | |||
|align="center"| per sample | |||
| >10uL of sample | |||
| Includes adapter ligation, barcoding, enrichment, and quality control (SYBR and FA). Insert sizes above 250bp. | |||
|- | |- | ||
| Full Service Sheared DNA (SPRIWORKS) | | Full Service Sheared DNA (SPRIWORKS) | ||
Line 139: | Line 146: | ||
|align="right"| $195 | |align="right"| $195 | ||
|align="center"| per sample | |align="center"| per sample | ||
| Includes adapter ligation, size selection, barcoding, enrichment, and quality control ( | | >10uL of sample | ||
| Includes adapter ligation, size selection, barcoding, enrichment, and quality control (SYBR and FA). | |||
|- | |- | ||
| ''SPRI-TE use only'' | | ''SPRI-TE use only'' | ||
Line 146: | Line 154: | ||
|align="right"| N/A | |align="right"| N/A | ||
|align="center"| per sample | |align="center"| per sample | ||
| <100uL | |||
| User must provide all primers. Samples will be returned for amplification. | | User must provide all primers. Samples will be returned for amplification. | ||
|- | |- | ||
Line 153: | Line 162: | ||
|align="right"| $225 | |align="right"| $225 | ||
|align="center"| per sample | |align="center"| per sample | ||
| Includes clean up, tagmentation, | | >10ul >10ng/uL | ||
| Includes clean up, tagmentation, indexing, enrichment, and quality control (SYBR and FA). | |||
|- | |- | ||
| Low Input Intact Genomic DNA (NEXTERA-XT) - 1ng input | | rowspan="2" | Low Input Intact Genomic DNA (NEXTERA-XT) - 1ng input | ||
|align="right"| $100 | |align="right"| $100 | ||
|align="right"| $110 | |align="right"| $110 | ||
|align="right"| $150 | |align="right"| $150 | ||
|align="center"| | |align="center"| ''1'' Sample | ||
| Includes tagmentation, | | >10uL | ||
| rowspan="4" | Includes tagmentation, indexing, enrichment, and quality control (SYBR and FA). | |||
|- | |||
|align="right" style="color: blue;" | $1000 | |||
|align="right" style="color: blue;" | $1100 | |||
|align="right" style="color: blue;" | $1500 | |||
|align="center" style="color: blue;" | ''16'' <BR>(2 column)<BR> TECAN | |||
|style="color: blue;" | >7ul at 0.2ng/ul in Axygen plate | |||
|- | |||
| rowspan="2" | Low Input Intact Genomic DNA (NEXTERA-XT) - 80pg input | |||
|align="right" style="color: blue;" | $1500 | |||
|align="right" style="color: blue;" | $1650 | |||
|align="right" style="color: blue;" | $2250 | |||
|align="center" style="color: blue;" | ''96'' <BR> Mosquito | |||
|style="color: blue;" | >5ul at 0.2ng/ul in Axygen plate | |||
|- | |||
|align="right" style="color: blue;" | $3000 | |||
|align="right" style="color: blue;" | $3300 | |||
|align="right" style="color: blue;" | $4500 | |||
|align="center" style="color: blue;" | ''384'' <BR> Mosquito | |||
|style="color: blue;" | >5ul at 0.2ng/ul in Axygen plate | |||
|- | |||
|style="color: blue;" | 16S Amplicon | |||
|align="right" style="color: blue;" | $1,800 | |||
|align="right" style="color: blue;" | $1,980 | |||
|align="right" style="color: blue;" | $2,700 | |||
|align="center"|48 samples <BR>(6 columns) | |||
|style="color: blue;" | >5ul, must amplify with Ct <20 by qPCR. | |||
|style="color: blue;" | Includes normalization, enrichment, pooling and quality control (SYBR and FA). | |||
|- | |||
|style="color: blue;" | Other Amplicon | |||
|align="right" style="color: blue;" | $1,440 | |||
|align="right" style="color: blue;" | $1,584 | |||
|align="right" style="color: blue;" | $2,160 | |||
|align="center" style="color: blue;" |48 samples <BR>(6 columns) | |||
|style="color: blue;" | >5ul, must amplify with Ct <20 by qPCR. | |||
|style="color: blue;" | Includes normalization, enrichment, pooling and quality control (SYBR and FA). <BR> Samples *must* be submitted amplified with appropriate linkers. | |||
|- | |||
| colspan="7" | '''ADDITIONAL FEES''' <BR> Samples not in proper input format will be subject to these additional charges. | |||
|- | |||
|style="color: blue;" | High Throughput Setup | |||
|align="right" style="color: blue;" | $50 | |||
|align="right" style="color: blue;" | $55 | |||
|align="right" style="color: blue;" | $75 | |||
|align="center" style="color: blue;" |48 samples | |||
| | |||
| | |||
|- | |- | ||
| | |Sample Cleanup | ||
|align="right"| $ | |align="right"| $5 | ||
|align="right"| $ | |align="right"| $5.5 | ||
|align="right"| $ | |align="right"| $7.5 | ||
|align="center"| per | |align="center"|per sample | ||
| | | | ||
|SPRI cleanup of samples to remove impurities. | |||
|- | |- | ||
| | |style="color: blue;" |Quantification | ||
|align="right"| $ | |align="right" style="color: blue;" | $30 | ||
|align="right"| $ | |align="right" style="color: blue;" | $33 | ||
|align="right"| $ | |align="right" style="color: blue;" | $45 | ||
|align="center"| | |align="center" style="color: blue;" |12 samples | ||
| | | | ||
|Quantification of samples (FA) prior to arraying. | |||
|- | |- | ||
|} | |||
| | |||
| | |||
=== SAMPLE PREPARATION - RNA === | |||
{| border=1 | |||
! ILLUMINA Sample Preparation | |||
!width=80| [[BioMicroCenter:CoreDeps|CORE LAB]] | |||
!width=80| MIT | |||
!width=80| [[BioMicroCenter:FAQ#NON_MIT_USERS|Non-MIT]] | |||
!width=160| unit | |||
!width=160| INPUT REQUIREMENT | |||
! Notes | |||
|- | |- | ||
| colspan=" | | colspan="7" | '''STANDARD RNA''' <BR> Prices for individual samples. Repreps will typically be attempted on failed samples without charge. | ||
|- | |- | ||
| Illumina | | colspan="7" style="color: blue;" | '''HIGH THROUGHPUT RNA''' <BR> Prices for individual samples. Repreps will NOT be attempted on failed samples without charge. Low volume (<=5ul) submission must be coordinated with BMC staff. | ||
|align="right"| $ | |- | ||
|align="right"| $ | | Illumina NEOPREP <BR> * Neoprep will be retired 7/17 | ||
|align="right"| $ | |align="right"| $100 | ||
|align="right"| $110 | |||
|align="right"| $150 | |||
|align="center"| per sample | |||
| | |||
| Performed using Neoprep system. <br> Requires polyA+ total RNA as input. No other inputs allowed. <BR>Includes purifying mRNA, generation of fragmented dsDNA, adaptor ligation, size selection, barcoding, enrichment, and quality control (RT-PCR and BioAnalyzer) | |||
|- | |||
|rowspan="2" | Standard polyA based isolation <BR> Kapa mRNA Hyperprep | |||
|align="right"| $150 | |||
|align="right"| $165 | |||
|align="right"| $225 | |||
|align="center"| per sample | |align="center"| per sample | ||
| | | >10ul, >10ng/ul | ||
|Includes QC, polyA isolation, generation of cDNA, library construction, indexing and quality control (SYBR + FA). | |||
|- | |||
|align="right" style="color: blue;" | $2000 | |||
|align="right" style="color: blue;" | $2200 | |||
|align="right" style="color: blue;" | $3000 | |||
|align="center" style="color: blue;" | ''24'' <br> TECAN | |||
| >10ul, >10ng/ul | |||
|Includes polyA isolation, generation of cDNA, library construction, indexing and quality control (SYBR + FA). | |||
|- | |||
| rowspan="2" style="color: blue;" | High Throughput 3' Digital Gene Expression (HT3DGE) | |||
|align="right" style="color: blue;" | $1200 | |||
|align="right" style="color: blue;" | $1320 | |||
|align="right" style="color: blue;" | $1800 | |||
|align="center" style="color: blue;" | ''24'' <br> TECAN | |||
| rowspan="2" style="color: blue;" | 5ul at 4 ng/uL of RIN7+ polyA+ RNA. | |||
| rowspan="2" style="color: blue;" | Includes generation of cDNA, library construction, indexing and library quality control. Does not include sample QC. | |||
|- | |||
|align="right" style="color: blue;" | $4000 | |||
|align="right" style="color: blue;" | $4400 | |||
|align="right" style="color: blue;" | $6000 | |||
|align="center" style="color: blue;" | ''96'' <br> TECAN | |||
|- | |- | ||
| | |Total RNA sequencing:Mammalian <BR> Kapa Hyperprep + RiboErase | ||
|align="right"| $ | |align="right"| $225 | ||
|align="right"| $ | |align="right"| $247.5 | ||
|align="right"| $ | |align="right"| $337.5 | ||
|align="center"| per sample | |align="center"| per sample | ||
| | | >10ul of >50ng/ul RNA | ||
| Includes sample QC, ribosomal depletion, generation of cDNA, library construction, indexing and library quality control. | |||
|- | |- | ||
| | |Total RNA sequencing:Other <BR> Kapa mRNA Hyperprep + Illumina RiboZero | ||
|align="right"| $250 | |||
|align="right"| $275 | |||
|align="right"| $375 | |align="right"| $375 | ||
|align="center"| per sample | |align="center"| per sample | ||
| | | >10ul of >100ng/ul RNA | ||
| Includes sample QC, ribosomal depletion, generation of cDNA, library construction, indexing and library quality control. | |||
|- | |- | ||
| Low Input ( | |rowspan="4"| Low Input polyA+ <BR> Clontech SMARTseq v4 + NexteraXT | ||
|align="right"| $ | |align="right"| $300 | ||
|align="right"| $ | |align="right"| $330 | ||
|align="right"| $ | |align="right"| $450 | ||
|align="center"| | |align="center"| ''1'' sample | ||
| | | >10ul of any input. | ||
| rowspan="4" | Includes generation of cDNA, amplification, library construction, indexing and quality control (SYBR + FA). Single sample includes initial QC. | |||
|- | |||
|align="right" style="color: blue;" | $1600 | |||
|align="right" style="color: blue;" | $1760 | |||
|align="right" style="color: blue;" | $2400 | |||
|align="center" style="color: blue;" | ''24'' <BR> Mosquito | |||
| style="color: blue;" | 5ul in axygen 96w plate | |||
|- | |||
|align="right" style="color: blue;" | $2500 | |||
|align="right" style="color: blue;" | $2750 | |||
|align="right" style="color: blue;" | $3750 | |||
|align="center" style="color: blue;" | ''96'' <BR> Mosquito | |||
| style="color: blue;" | 5ul in axygen 96w plate | |||
|- | |||
|align="right" style="color: blue;" | $5000 | |||
|align="right" style="color: blue;" | $5500 | |||
|align="right" style="color: blue;" | $7500 | |||
|align="center" style="color: blue;" | ''384'' <BR> Mosquito | |||
| style="color: blue;" | 5ul in axygen 96w plates | |||
|- | |||
|rowspan="1"| Low Input Mammalian Total <BR> Clontech ZapR Kit | |||
|align="right"| $200 | |||
|align="right"| $220 | |||
|align="right"| $300 | |||
|align="center"| ''1'' sample | |||
| >5ul of any input. | |||
| rowspan="1" | Includes generation of cDNA, amplification, library construction, rRNA degradation, indexing and quality control (SYBR + FA). Single sample includes initial QC. | |||
|- | |- | ||
| Tight Size Cut | | Tight Size Cut | ||
Line 218: | Line 344: | ||
|align="right"| $130 | |align="right"| $130 | ||
|align="center"| per pool | |align="center"| per pool | ||
| | |||
| +/- 40bp using PippinPrep | | +/- 40bp using PippinPrep | ||
|- | |- | ||
Line 225: | Line 352: | ||
| align="right"|$413 | | align="right"|$413 | ||
| align="center"|per sample | | align="center"|per sample | ||
| Uses NEB small RNA kit | | | ||
| Uses NEB, BIOO or QIAGEN small RNA kit | |||
|- | |||
| colspan="7" | '''ADDITIONAL FEES''' <BR> Samples not in proper input format will be subject to these additional charges. | |||
|- | |||
|style="color: blue;" | High Throughput Setup | |||
|align="right" style="color: blue;" | $50 | |||
|align="right" style="color: blue;" | $55 | |||
|align="right" style="color: blue;" | $75 | |||
|align="center" style="color: blue;" |48 samples | |||
| | |||
| | |||
|- | |- | ||
| colspan="6" | | |Sample Cleanup | ||
|align="right"| $5 | |||
|align="right"| $5.5 | |||
|align="right"| $7.5 | |||
|align="center"|per sample | |||
| | |||
|SPRI cleanup of samples to remove impurities. | |||
|- | |||
|style="color: blue;" |Quantification | |||
|align="right" style="color: blue;" | $30 | |||
|align="right" style="color: blue;" | $33 | |||
|align="right" style="color: blue;" | $45 | |||
|align="center" style="color: blue;" |12 samples | |||
| | |||
|Quantification of samples (FA) prior to arraying. | |||
|- | |||
|} | |||
=== SAMPLE PREPARATION - CHROMATIN === | |||
{| border=1 | |||
! ILLUMINA Sample Preparation | |||
!width=80| [[BioMicroCenter:CoreDeps|CORE LAB]] | |||
!width=80| MIT | |||
!width=80| [[BioMicroCenter:FAQ#NON_MIT_USERS|Non-MIT]] | |||
!width=80| unit | |||
! Notes | |||
|- | |||
| colspan="6" | Note: User must supply antibodies. | |||
|- | |- | ||
| Full Service ChIP-seq | | Full Service ChIP-seq | ||
Line 244: | Line 410: | ||
|- | |- | ||
| Covaris Sonicator | | Covaris Sonicator | ||
|align="right"| $15 | |align="right"| $15 | ||
|align="right"| $20 | |||
|align="right"| NA | |align="right"| NA | ||
|align="center"| per hour | |align="center"| per hour | ||
Line 253: | Line 419: | ||
. | . | ||
== [[BioMicroCenter:MicroArrays|MICROARRAYS]] == | == [[BioMicroCenter:MicroArrays|MICROARRAYS]] == | ||
Line 332: | Line 493: | ||
| AA - Preloaded plate | | AA - Preloaded plate | ||
|align="right"| $30 | |align="right"| $30 | ||
|align="right"| | |align="right"| N/A | ||
|align="center"| per row (12) | |align="center"| per row (12) | ||
| 2ul per sample in each well using plates from BMC | | 2ul per sample in each well using plates from BMC | ||
Line 361: | Line 522: | ||
! SIGN UP | ! SIGN UP | ||
! Notes | ! Notes | ||
|- | |- | ||
| Roche LightCycler 480 | | Roche LightCycler 480 | ||
|align="right"| $10 | |align="right"| $10 | ||
|align="right"| | |align="right"| N/A | ||
|align="center"| per plate | |align="center"| per plate | ||
| [[BioMicroCenter:Forms#Calenders|CALENDER]] *available for CORE only | | [[BioMicroCenter:Forms#Calenders|CALENDER]] *available for CORE only | ||
Line 393: | Line 531: | ||
|- | |- | ||
| SYBR Green (KAPA/Roche) | | SYBR Green (KAPA/Roche) | ||
|align="right"| $ | |align="right"| $36 | ||
|align="right"| | |align="right"| N/A | ||
|align="center"| per ml | |align="center"| per ml | ||
| | | | ||
Line 401: | Line 539: | ||
| 96-well PLATES | | 96-well PLATES | ||
|align="right"| $6 | |align="right"| $6 | ||
|align="right"| | |align="right"| N/A | ||
|align="center"| per plate | |align="center"| per plate | ||
| | | | ||
Line 407: | Line 545: | ||
|- | |- | ||
|} | |} | ||
. | . | ||
<br> | <br> | ||
Line 440: | Line 577: | ||
|- | |- | ||
| Varioskan | | Varioskan | ||
|align="right"| $18 | |align="right"| $18 | ||
|align="right"| $27 | |||
|align="center"| per hour | |align="center"| per hour | ||
| [[BioMicroCenter:Forms#Calenders|CALENDER]] | | [[BioMicroCenter:Forms#Calenders|CALENDER]] | ||
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| Tecan EVO 150 | | Tecan EVO 150 | ||
|align="right"| $ | |align="right"| $50 | ||
|align="right"| $ | |align="right"| $55 | ||
|align="center"| per | |align="center"| per 1h block - based on scheduled time. | ||
| [[BioMicroCenter:Forms#Calenders|CALENDER]] | | [[BioMicroCenter:Forms#Calenders|CALENDER]] | ||
| | | | ||
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|- | |- | ||
| Training | | Training | ||
|align="right"| $ | |align="right"| $50 | ||
|align="right"| $ | |align="right"| $55 | ||
|align="center"| per session | |align="center"| per session | ||
| Contact [[BioMicroCenter:People|Stuart]] | | Contact [[BioMicroCenter:People|Stuart]] | ||
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|} | |} | ||
<br><br> | <br><br> | ||
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| Informatics Support | | Informatics Support | ||
| align="right"| $ | | align="right"| $75 | ||
| align="right"| $ | | align="right"| $100 / $130 | ||
| align="right"| per hour | | align="right"| per hour | ||
| Limited support for non-MIT users | | Limited support for non-MIT users | ||
|- | |- | ||
| Informatics Share | | Informatics Share | ||
| align="right"| $ | | align="right"| $1,000 | ||
| align="right"| N/A | | align="right"| N/A | ||
| align="right"| per 0.1FTE | | align="right"| per 0.1FTE | ||
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|- | |- | ||
| | | Archival Storage (1 year - read only. Unmutable) | ||
|align="right"| $ | |align="right"| $140 | ||
|align="right"| $ | |align="right"| $200 / N/A | ||
|align="center"| per TB | |align="center"| per TB | ||
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[[BioMicroCenter:PricingFY2017 | FY2017 Pricing]]<BR> | |||
[[BioMicroCenter:PricingFY2016 | FY2016 Pricing]]<BR> | [[BioMicroCenter:PricingFY2016 | FY2016 Pricing]]<BR> | ||
[[BioMicroCenter:PricingFY2015 | FY2015 Pricing]]<BR> | [[BioMicroCenter:PricingFY2015 | FY2015 Pricing]]<BR> |
Revision as of 11:07, 26 April 2017
HOME -- | SEQUENCING -- | LIBRARY PREP -- | HIGH-THROUGHPUT -- | COMPUTING -- | OTHER TECHNOLOGY |
SEQUENCING
Please note that samples submitted from CORE LABs will be given priority on the HiSeq.
ILLUMINA SEQUENCING | CORE LAB | MIT | Non-MIT | unit | Notes |
---|---|---|---|---|---|
HiSEQ 2000 sequencing (minimum 150m reads/lane*. Average ~160-240m reads/lane) | |||||
Single End (40nt) | $1,140 | $1,254 | $1,710 | per lane | Includes final pool quality control (RT-PCR and BioAnalyzer), sequencing, genome alignment and data storage of BAM files for 1y. |
Paired End (40+40nt) | $1,710 | $1,881 | $2,565 | per lane | |
Add'l 40nt | $330 | $363 | $495 | per lane | |
NextSeq sequencing (minimum 250m reads/lane. maximum ~500m reads/lane) | |||||
91nt kit (eg. 40PE or 50SE) |
$2,100 | $2,310 | $3,150 | per flowcell | Includes final pool quality control (RT-PCR and BioAnalyzer), sequencing, genome alignment and data storage of BAM files for 1 year ** THESE RUNS WILL HAVE LOW PRIORITY |
166nt kit (eg. 80PE or 150SE) |
$3,150 | $3,465 | $4,725 | per flowcell | Includes final pool quality control (RT-PCR and BioAnalyzer), sequencing, genome alignment and data storage of BAM files for 1 year |
316nt kit (eg. 150PE or 300SE) |
$4,725 | $5,200 | $7,088 | per flowcell | Includes final pool quality control (RT-PCR and BioAnalyzer), sequencing, genome alignment and data storage of BAM files for 1 year |
MiSeq v2 sequencing (minimum 8m reads/lane. maximum ~15m reads/lane) | |||||
70nt Run (eg. 30PE or 70SE) |
$1,155 | $1,270 | $1,732 | per lane | Includes final pool quality control (RT-PCR and BioAnalyzer), sequencing, genome alignment and data storage of BAM files for 1 year |
300nt Run (eg. 150PE) |
$1,365 | $1,500 | $2,050 | per lane | Includes final pool quality control (RT-PCR and BioAnalyzer), sequencing, genome alignment and data storage of BAM files for 1 year |
500nt Run (eg. 250PE) |
$1,575 | $1,732 | $2,362 | per lane | Includes final pool quality control (RT-PCR and BioAnalyzer), sequencing, genome alignment and data storage of BAM files for 1 year |
MiSeq v3 sequencing (minimum 15m reads/lane. Expected ~25m reads/lane) | |||||
150nt Run (eg. 75PE) |
$1,365 | $1,500 | $2,050 | per lane | Includes final pool quality control (RT-PCR and BioAnalyzer), sequencing, genome alignment and data storage of BAM files for 1 year |
600nt Run (eg. 300PE) |
$1,890 | $2,080 | $2,835 | per lane | Includes final pool quality control (RT-PCR and BioAnalyzer), sequencing, genome alignment and data storage of BAM files for 1 year |
Additional Services | |||||
These services apply to samples submitted for all platforms. | |||||
Quality Control Only -only one QC is included per sequencing lane. | $20 | $26 | $30 | per sample | Includes RT-PCR and Frag.Analyzer |
Rapid Quality Control - Use SYBR for sample quantification for pooling instead of qPCR. Only available for pooling. | $10 | $13 | $15 | per sample | Includes Fluorometric quantification and Frag.Analyzer |
Please note that samples submitted from CORE LABs will be given priority on all equipment. The BioMicro Center reserves the right to reject samples for any reason.
LIBRARY GENERATION
SAMPLE PREPARATION - DNA
ILLUMINA Sample Preparation | CORE LAB | MIT | Non-MIT | unit | INPUT REQUIREMENT | Notes |
---|---|---|---|---|---|---|
STANDARD THROUGHPUT DNA Prices for individual samples. Repreps will typically be attempted on failed samples without charge. |
HIGH THROUGHPUT DNA Prices for individual samples. Repreps will NOT be attempted on failed samples without charge. Low volume (<=5ul) submission must be coordinated with BMC staff. | |||||
NEOPREP DNA * Neoprep will be retired 7/17 |
$70 | $77 | $105 | per sample | >10uL of sample | Includes adapter ligation, barcoding, enrichment, and quality control (SYBR and FA). Insert sizes above 250bp. |
Full Service Sheared DNA (SPRIWORKS) | $130 | $143 | $195 | per sample | >10uL of sample | Includes adapter ligation, size selection, barcoding, enrichment, and quality control (SYBR and FA). |
SPRI-TE use only | $85 | $95 | N/A | per sample | <100uL | User must provide all primers. Samples will be returned for amplification. |
Intact Genomic DNA (NEXTERA) - 50ng input | $150 | $165 | $225 | per sample | >10ul >10ng/uL | Includes clean up, tagmentation, indexing, enrichment, and quality control (SYBR and FA). |
Low Input Intact Genomic DNA (NEXTERA-XT) - 1ng input | $100 | $110 | $150 | 1 Sample | >10uL | Includes tagmentation, indexing, enrichment, and quality control (SYBR and FA). |
$1000 | $1100 | $1500 | 16 (2 column) TECAN |
>7ul at 0.2ng/ul in Axygen plate | ||
Low Input Intact Genomic DNA (NEXTERA-XT) - 80pg input | $1500 | $1650 | $2250 | 96 Mosquito |
>5ul at 0.2ng/ul in Axygen plate | |
$3000 | $3300 | $4500 | 384 Mosquito |
>5ul at 0.2ng/ul in Axygen plate | ||
16S Amplicon | $1,800 | $1,980 | $2,700 | 48 samples (6 columns) |
>5ul, must amplify with Ct <20 by qPCR. | Includes normalization, enrichment, pooling and quality control (SYBR and FA). |
Other Amplicon | $1,440 | $1,584 | $2,160 | 48 samples (6 columns) |
>5ul, must amplify with Ct <20 by qPCR. | Includes normalization, enrichment, pooling and quality control (SYBR and FA). Samples *must* be submitted amplified with appropriate linkers. |
ADDITIONAL FEES Samples not in proper input format will be subject to these additional charges. | ||||||
High Throughput Setup | $50 | $55 | $75 | 48 samples | ||
Sample Cleanup | $5 | $5.5 | $7.5 | per sample | SPRI cleanup of samples to remove impurities. | |
Quantification | $30 | $33 | $45 | 12 samples | Quantification of samples (FA) prior to arraying. |
SAMPLE PREPARATION - RNA
ILLUMINA Sample Preparation | CORE LAB | MIT | Non-MIT | unit | INPUT REQUIREMENT | Notes |
---|---|---|---|---|---|---|
STANDARD RNA Prices for individual samples. Repreps will typically be attempted on failed samples without charge. | ||||||
HIGH THROUGHPUT RNA Prices for individual samples. Repreps will NOT be attempted on failed samples without charge. Low volume (<=5ul) submission must be coordinated with BMC staff. | ||||||
Illumina NEOPREP * Neoprep will be retired 7/17 |
$100 | $110 | $150 | per sample | Performed using Neoprep system. Requires polyA+ total RNA as input. No other inputs allowed. Includes purifying mRNA, generation of fragmented dsDNA, adaptor ligation, size selection, barcoding, enrichment, and quality control (RT-PCR and BioAnalyzer) | |
Standard polyA based isolation Kapa mRNA Hyperprep |
$150 | $165 | $225 | per sample | >10ul, >10ng/ul | Includes QC, polyA isolation, generation of cDNA, library construction, indexing and quality control (SYBR + FA). |
$2000 | $2200 | $3000 | 24 TECAN |
>10ul, >10ng/ul | Includes polyA isolation, generation of cDNA, library construction, indexing and quality control (SYBR + FA). | |
High Throughput 3' Digital Gene Expression (HT3DGE) | $1200 | $1320 | $1800 | 24 TECAN |
5ul at 4 ng/uL of RIN7+ polyA+ RNA. | Includes generation of cDNA, library construction, indexing and library quality control. Does not include sample QC. |
$4000 | $4400 | $6000 | 96 TECAN | |||
Total RNA sequencing:Mammalian Kapa Hyperprep + RiboErase |
$225 | $247.5 | $337.5 | per sample | >10ul of >50ng/ul RNA | Includes sample QC, ribosomal depletion, generation of cDNA, library construction, indexing and library quality control. |
Total RNA sequencing:Other Kapa mRNA Hyperprep + Illumina RiboZero |
$250 | $275 | $375 | per sample | >10ul of >100ng/ul RNA | Includes sample QC, ribosomal depletion, generation of cDNA, library construction, indexing and library quality control. |
Low Input polyA+ Clontech SMARTseq v4 + NexteraXT |
$300 | $330 | $450 | 1 sample | >10ul of any input. | Includes generation of cDNA, amplification, library construction, indexing and quality control (SYBR + FA). Single sample includes initial QC. |
$1600 | $1760 | $2400 | 24 Mosquito |
5ul in axygen 96w plate | ||
$2500 | $2750 | $3750 | 96 Mosquito |
5ul in axygen 96w plate | ||
$5000 | $5500 | $7500 | 384 Mosquito |
5ul in axygen 96w plates | ||
Low Input Mammalian Total Clontech ZapR Kit |
$200 | $220 | $300 | 1 sample | >5ul of any input. | Includes generation of cDNA, amplification, library construction, rRNA degradation, indexing and quality control (SYBR + FA). Single sample includes initial QC. |
Tight Size Cut | $85 | $94 | $130 | per pool | +/- 40bp using PippinPrep | |
Small RNA | $275 | $303 | $413 | per sample | Uses NEB, BIOO or QIAGEN small RNA kit | |
ADDITIONAL FEES Samples not in proper input format will be subject to these additional charges. | ||||||
High Throughput Setup | $50 | $55 | $75 | 48 samples | ||
Sample Cleanup | $5 | $5.5 | $7.5 | per sample | SPRI cleanup of samples to remove impurities. | |
Quantification | $30 | $33 | $45 | 12 samples | Quantification of samples (FA) prior to arraying. |
SAMPLE PREPARATION - CHROMATIN
ILLUMINA Sample Preparation | CORE LAB | MIT | Non-MIT | unit | Notes |
---|---|---|---|---|---|
Note: User must supply antibodies. | |||||
Full Service ChIP-seq | $210 | $231 | $315 | per sample | Performed using IP-Star AND SPRIworks system. Includes ChIP, library contruction and barcoding. |
IP-Star Only | $300 | $330 | $450 | per batch (1-8 samples) | Performed using IP-Star |
Covaris Sonicator | $15 | $20 | NA | per hour | Training Required |
.
MICROARRAYS
Microarray processing prices do not include the cost of the array. In order to be sure we have arrays on hand, we encourage users to contact us several days in advance of submitting samples.
MICROARRAYS | CORE LAB | MIT | Non-MIT | unit | Notes |
---|---|---|---|---|---|
AFFYMETRIX PROCESSING | |||||
Eukaryote (3' or Exon: >20ng) | $200 | $250 | $325 | per sample | Using AFFYMETRIX sample labeling kit. For NuGEN reagents, please refer to 2015 pricing. |
Eukaryote (<20ng) | $400 | $500 | $750 | per sample | Using NuGEN sample labeling kit. |
Prokaryote | $365 | $455 | $683 | per sample | Using Ambion sample labeling kit. |
AGILENT PROCESSING | |||||
Agilent array services are being phased out in FY2016. Requests that must be processed on Agilent will be priced per experiment. | |||||
Scanning Only | $50 | $50 | N/A | per slide | Price per glass slide. |
prices effective 7.1.2015
Common Affymetrix Arrays* (some may be in stock):
$175 - Prokaryote/Small Eukaryote E.coli Genome $250 - Eukaryote 3' (low density) Human U133A 2.0 / Mouse 430A 2.0 $400 - Eukaryote Exon or 3' (high density) Human U133+ 2.0 / Mouse 430 2.0 / Human or Mouse Exon 1.0 ST Full Price List: Affymetrix Prices
- available at cost to academic labs only. shipping fees may be added. Non-academic labs should order their own arrays and have them shipped to the BioMicro Center.
AGILENT BIOANALYZER
AGILENT BIOANALYZER / ADVANCED ANALYTICAL-FRAGMENT ANALYZER | CORE LAB / MIT | Non-MIT | unit | Notes |
---|---|---|---|---|
RNA / DNA on Advanced Analytical | $8 | $12 | per sample | |
AA - Preloaded plate | $30 | N/A | per row (12) | 2ul per sample in each well using plates from BMC |
All DNA & RNA on BIOANALYZER | $15 | $25 | per sample | |
High Sensitivity protein | $40 | $60 | per sample | detection of proteins at < 30ng/ul. |
prices effective 7.1.2015
.
REAL TIME PCR
OTHER LAB SERVICES | CORE LAB / MIT | Non-MIT | unit | SIGN UP | Notes |
---|---|---|---|---|---|
Roche LightCycler 480 | $10 | N/A | per plate | CALENDER *available for CORE only | Contact Jon Penterman for training |
SYBR Green (KAPA/Roche) | $36 | N/A | per ml | Available for MIT only | |
96-well PLATES | $6 | N/A | per plate | Available for MIT only |
.
OTHER LAB SERVICES
OTHER LAB SERVICES | CORE LAB / MIT | Non-MIT | unit | SIGN UP | Notes |
---|---|---|---|---|---|
PIPPIN PREP | |||||
Gel Isolation | $50 | $75 | per gel (5 lanes) | TBA | |
Training | $25 | $50 | per session | TBA | Contact Jon Penterman for training. |
PLATE READER | |||||
Varioskan | $18 | $27 | per hour | CALENDER | Contact Jon Penterman for training |
ROBOTICS | |||||
Tecan EVO 150 | $50 | $55 | per 1h block - based on scheduled time. | CALENDER | |
non-filter tips | $6.00 | $6.00 | per box | ** Please let us know if there is additional plasticware you would like us to stock. | |
Filter tips | $8.00 | $8.00 | per box | ||
Training | $50 | $55 | per session | Contact Stuart | |
NANODROP | |||||
Nanodrop | -- | -- | Walk up service | * no charge |
COMPUTATIONAL SERVICES
COMPUTATIONAL SERVICES | CORE LAB | Other MIT / Non-MIT | unit | Notes |
---|---|---|---|---|
Informatics Support | $75 | $100 / $130 | per hour | Limited support for non-MIT users |
Informatics Share | $1,000 | N/A | per 0.1FTE | MIT only. Priority access to informatics. |
Data storage (1 year) | $280 | $400 / N/A | per TB | |
Archival Storage (1 year - read only. Unmutable) | $140 | $200 / N/A | per TB | |
Tape Archive / Recovery | $60 | $70 | per tape |
FY2017 Pricing
FY2016 Pricing
FY2015 Pricing
FY2014 Pricing
FY2013 Pricing
FY2012 Pricing
FY2011 Pricing