BioMicroCenter:Pricing

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ILLUMINA SEQUENCING

SEQUENCING

Please note that samples submitted from CORE LABs will be given priority on the Genome Analyzer.

ILLUMINA SEQUENCING	CORE LAB/MIT	Non-MIT	unit	Notes
GAII / GAIIx sequencing (minimum 10m reads/lane. Average ~25-30m reads/lane)
Single End (36nt)	$810	$1,215	per lane	Includes quality control (RT-PCR and BioAnalyzer), sequencing, genome alignment and data storage of Firecrest files for 2 yrs.
Paired End (36+36nt)	$1,450	$2,175	per lane
Add'l 36nt	$300	$450	per lane
HiSEQ 2000 sequencing (minimum 25m reads/lane. Average ~75-100m reads/lane)
Single End (40nt)	$1,170	$1,755	per lane	Includes quality control (RT-PCR and BioAnalyzer), sequencing, genome alignment and data storage of Firecrest files for 2 yrs.
Paired End (40+40nt)	$1,850	$2,775	per lane
Add'l 40nt	$370	$555	per lane
Additional Services
Sample Multiplexing	$50	$75	per 10 samples	Combined over the whole order. Only applies to samples combined by BMC technicians.
Add'l Repriming	$200	$300	per flow cell	Uses 6nt. Cost is divided by number of lanes requiring repriming. Repriming is required for standard Illumina multiplexing.
Quality Control Only	$40	$60	per sample

Prices effective 7.1.2011

Please note that samples submitted from CORE LABs will be given priority on the Genome Analyzer. The BioMicro Center reserves the right to reject samples for sample preparations.

SAMPLE PREPARATION

ILLUMINA Sample Preparation	CORE LAB/MIT	Non-MIT	unit	Notes
Full Service Sheared DNA	$170	$255	per sample	Includes adapter ligation, size selection, barcoding, enrichment, and quality control (RT-PCR and BioAnalyzer).
SPRI-TE use only	$110	N/A	per sample	User must provide all primers. Samples will be returned for amplification.
Full Service Intact Genomic DNA	$200	$300	per sample	Preformed using NEXTERA system. Includes adapter ligation, size selection, barcoding, enrichment, and quality control (RT-PCR and BioAnalyzer).
Full Service RNA-seq	$350	$525	per sample	Preformed using TruSeq AND SPRIworks system. Includes purifying mRNA, generation of fragmented dsDNA, adaptor ligation, size selection, barcoding, enrichment, and quality control (RT-PCR and BioAnalyzer).

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MICROARRAYS

Microarray processing prices do not include the cost of the array. In order to be sure we have arrays on hand, we encourage users to contact us several days in advance of submitting samples.

MICROARRAYS	CORE LAB	MIT	Non-MIT	unit	Notes
AFFYMETRIX PROCESSING
Eukaryote (3' or Exon: >20ng)	$265	$350	$525	per sample	Using NuGEN sample labeling kit.
Eukaryote (<20ng)	$375	$470	$700	per sample	Using NuGEN sample labeling kit.
Prokaryote	$360	$450	$675	per sample	Using Ambion sample labeling kit.
Fluidics Station and Scan	$130	$165	N/A	per array	Users must be trained and coordinate with Manlin.
AGILENT PROCESSING
Expression - ONE COLOR - Eukaryote	$175	$235	$350	per array
Expression - TWO COLOR - Eukaryote	$250	$300	$450	per array	Includes both Cy5 and Cy3
miRNA - ONE COLOR	$230	$300	$450	per array
Prokaryote - ONE COLOR	$230	$300	$450	per array
Scanning Only	$50	$50	N/A	per slide	Price per glass slide.

prices effective 7.1.2011

Common Affymetrix Arrays* (some may be in stock):

$175  - Prokaryote/Small Eukaryote                E.coli Genome 
$250  - Eukaryote 3' (low density)                Human U133A 2.0 / Mouse 430A 2.0
$400  - Eukaryote Exon or 3' (high density)       Human U133+ 2.0 / Mouse 430 2.0 / Human or Mouse Exon 1.0 ST
Full Price List:             Affymetrix Prices

Common Agilent Expression Arrays*^:

$807.20  - 8x15k designs (cost per tile, must use all 8 at once)       E.coli / S.cerevisiae / Custom
$170     - 4x44k designs (cost per tile, must use all 4 at once)       Human / Mouse / C.elegans / Custom

• available at cost to academic labs only. shipping fees may be added. Non-academic labs should order their own arrays and have them shipped to the BioMicro Center.

^ Discount pricing available through the BioMicro Center.

AGILENT BIOANALYZER

AGILENT BIOANALYZER	CORE LAB / MIT	Non-MIT	unit	Notes
All DNA and RNA samples	$15	$25	per sample
High Sensitivity protein	$40	$60	per sample	detection of proteins at < 30ng/ul.
Flow Cytometry	by request	NA	per sample

prices effective 7.1.2011
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REAL TIME PCR

OTHER LAB SERVICES	CORE LAB / MIT	Non-MIT	unit	SIGN UP	Notes
FLUIDIGM
Fluidigm BioMark	$150	$225	per use	CALENDER	Contact Ryan
48.48 Dynamic Array Chip	$260	N/A	per cassette	Cassettes are available in the BMC.	Contact Ryan
96.96 Dynamic Array Chip	$760	N/A	per cassette	Cassettes are available in the BMC.	Contact Ryan
RT-PCR
Roche LightCycler 480	$10	NA	per plate	CALENDER *available for CORE only	Contact Michael for training
MJ Opticon II	$10	$15	per plate	CALENDER	Contact Manlin for training
SYBR Green (KAPA/Roche)	$34	NA	per ml		Available for MIT only
96-well RTPCR PLATE	$6	NA	per plate		Available for MIT only

prices effective 7.1.2011 .

OTHER LAB SERVICES

OTHER LAB SERVICES	CORE LAB / MIT	Non-MIT	unit	SIGN UP	Notes
PLATE READER
Varioskan	$10	$15	per plate	CALENDER	Quick scans (those requiring less then 30 minutes) are free of charge but may not be scheduled on the calender. Users who have large experiments have priority. Contact Kevin for training
ROBOTICS
Tecan EVO 150	$50	$150	per 2h block - based on scheduled time.	CALENDER
non-filter tips	$5.00	$5.00	per box		** Please let us know if there is additional plasticware you would like us to stock.
Filter tips	$8.00	$8.00	per box
Training	$100	$150	per session	Contact Barbara
NANODROP
Nanodrop	--	--		Walk up service	* no charge

prices effective 7.1.2011

COMPUTATIONAL SERVICES

COMPUTATIONAL SERVICES	CORE LAB	Other MIT / Non-MIT	unit	Notes
Data storage (1 year)	$500	N/A	per TB	(Limited space available)
Spotfire License (1 year)	Contact Stephen Goldman	N/A	per user	(Limited seats available)

FY2011 Pricing