BioMicroCenter:PricingFY2015

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ILLUMINA SEQUENCING

DRAFT - NOT FOR USE

SEQUENCING

Please note that samples submitted from CORE LABs will be given priority on the HiSeq.

ILLUMINA SEQUENCING	CORE LAB	MIT	Non-MIT	unit	Notes
HiSEQ 2000 sequencing (minimum 150m reads/lane*. Average ~160-240m reads/lane)
Single End (40nt)	$1,085	$1,195	$1,628	per lane	Includes quality control (RT-PCR and BioAnalyzer), sequencing, genome alignment and data storage of BAM files for 1y.
Paired End (40+40nt)	$1,625	$1,788	$2,438	per lane
Add'l 40nt	$300	$330	$450	per lane
MiSeq v2 sequencing (minimum 8m reads/lane. maximum ~15m reads/lane)
70nt Run	$1,150	$1,265	$1,725	per lane	Includes quality control (RT-PCR and BioAnalyzer), sequencing, genome alignment and data storage of BAM files for 1 year
300nt Run (PE or SE)	$1,350	$1,485	$2,025	per lane	Includes quality control (RT-PCR and BioAnalyzer), sequencing, genome alignment and data storage of BAM files for 1 year
500nt Run (PE or SE)	$1,600	$1,760	$2,400	per lane	Includes quality control (RT-PCR and BioAnalyzer), sequencing, genome alignment and data storage of BAM files for 1 year
MiSeq v3 sequencing (minimum 15m reads/lane. Expected ~25m reads/lane)
600nt Run (PE or SE)	$2,050	$2,255	$3,075	per lane	Includes quality control (RT-PCR and BioAnalyzer), sequencing, genome alignment and data storage of BAM files for 1 year
GAII / GAIIx sequencing (Average ~25-40m reads/lane)
GAII Services are being phased out in FY2015. Requests that must be processed on the GAII will be priced per experiment.
Additional Services
These services apply to samples submitted for all platforms.
Sample Multiplexing	$50	$55	$75	per 10 samples	Combined over the whole order. Only applies to samples combined by BMC technicians.
Add'l Repriming (HiSeq only)	$200	$220	$300	per flow cell	Uses 6-8nt. Cost is divided by number of lanes requiring repriming. Repriming is required for standard Illumina multiplexing.
Quality Control Only	$25	$28	$38	per sample	Includes RT-PCR and BioAnalyzer

Please note that samples submitted from CORE LABs will be given priority on all equipment. The BioMicro Center reserves the right to reject samples for any reason.

SAMPLE PREPARATION

ILLUMINA Sample Preparation	CORE LAB	MIT	Non-MIT	unit	Notes
STARTING WITH DNA
Full Service Sheared DNA (SPRIWORKS)	$150	$165	$225	per sample	Includes adapter ligation, size selection, barcoding, enrichment, and quality control (RT-PCR and BioAnalyzer).
SPRI-TE use only	$100	$110	N/A	per sample	User must provide all primers. Samples will be returned for amplification.
Intact Genomic DNA (NEXTERA) - 50ng input	$175	$193	$263	per sample	Includes clean up, tagmentation, barcoding, enrichment, and quality control (RT-PCR and BioAnalyzer).
Low Input Intact Genomic DNA (NEXTERA-XT) - 1ng input	$100	$110	$150	per sample	Includes tagmentation, barcoding, enrichment, and quality control (RT-PCR and BioAnalyzer).
16S Amplicon	$1,200	$1,320	$1,800	per 48 sample	Includes normalization, enrichment, pooling and quality control (RT-PCR and BioAnalyzer).
Other Amplicon	$960	$1,056	$1,440	per 48 sample	Includes normalization, enrichment, pooling and quality control (RT-PCR and BioAnalyzer). Samples *must* be submitted amplified with appropriate linkers.
STARTING WITH RNA
Illumina TruSeq	$300	$330	$450	per sample	Preformed using TruSeq AND SPRIworks system. Includes purifying mRNA, generation of fragmented dsDNA, adaptor ligation, size selection, barcoding, enrichment, and quality control (RT-PCR and BioAnalyzer).
+strand specific	$30	$33	$45	per sample	Requires 1ug of RNA. Uses the dUTP method.
Epicenter Ribozero no polyA pulldown	$350	$385	$525	per sample	Requires 1ug of RNA. Based on Illumina Truseq chemistry
Low Input (Clontech)	$400	$440	$600	per sample	Can work on single cells
Low Input (NuGen)	$475	$523	$713	per sample	Can work on degraded RNA.
Tight Size Cut	$100	$110	$150	per pool	+/- 40bp using PippinPrep
Small RNA	$275	$303	$413	per sample	Uses NEB small RNA kit
STARTING WITH CHROMATIN Note: User must supply antibodies.
Full Service ChIP-seq	$210	$231	$315	per sample	Preformed using IP-Star AND SPRIworks system. Includes ChIP, library contruction and barcoding.
IP-Star Only	$300	$330	$450	per batch (1-8 samples)	Preformed using IP-Star

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MICROARRAYS

Microarray processing prices do not include the cost of the array. In order to be sure we have arrays on hand, we encourage users to contact us several days in advance of submitting samples.

MICROARRAYS	CORE LAB	MIT	Non-MIT	unit	Notes
AFFYMETRIX PROCESSING
Eukaryote (3' or Exon: >20ng)	$330	$410	$600	per sample	Using NuGEN sample labeling kit.
Eukaryote (<20ng)	$430	$530	$780	per sample	Using NuGEN sample labeling kit.
Prokaryote	$400	$480	$700	per sample	Using Ambion sample labeling kit.
AGILENT PROCESSING
Expression - ONE COLOR	$225	$275	$400	per array
Expression - TWO COLOR	$325	$375	$550	per array	Includes both Cy5 and Cy3
Scanning Only	$50	$50	N/A	per slide	Price per glass slide.

prices effective 7.1.2014

Common Affymetrix Arrays* (some may be in stock):

$175  - Prokaryote/Small Eukaryote                E.coli Genome 
$250  - Eukaryote 3' (low density)                Human U133A 2.0 / Mouse 430A 2.0
$400  - Eukaryote Exon or 3' (high density)       Human U133+ 2.0 / Mouse 430 2.0 / Human or Mouse Exon 1.0 ST
Full Price List:             Affymetrix Prices

• available at cost to academic labs only. shipping fees may be added. Non-academic labs should order their own arrays and have them shipped to the BioMicro Center.

AGILENT BIOANALYZER

AGILENT BIOANALYZER / ADVANCED ANALYTICAL-FRAGMENT ANALYZER	CORE LAB	MIT	Non-MIT	unit	Notes
RNA / DNA on Advanced Analytical	$8	$8	$12	per sample
AA - Preloaded plate	$30	$33	$45	per row (12)	2ul per sample in each well using plates from BMC
All DNA & RNA on BIOANALYZER	$15	$15	$25	per sample
High Sensitivity protein	$40	$45	$60	per sample	detection of proteins at < 30ng/ul.
Self run chips	$55-90	request	NA	per chip	Price varies by flavor of Chip.

prices effective 7.1.2014
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REAL TIME PCR

OTHER LAB SERVICES	CORE LAB MIT	MIT	Non-MIT	unit	SIGN UP	Notes
FLUIDIGM
Fluidigm BioMark	$150	$165	$225	per use	CALENDER	Contact Shmulik
48.48 Dynamic Array Chip	$250		N/A	per cassette	Cassettes are available in the BMC.
96.96 Dynamic Array Chip	$700		N/A	per cassette	Cassettes are available in the BMC.
WAFERGEN SMART CHIP	$150		N/A	per chip	Chips available from BMC	Contact Michael
WAFERGEN TRAINING	$100	$110	N/A	per chip	Mandatory	Contact Michael
RT-PCR
Roche LightCycler 480	$15	$20	NA	per plate	CALENDER *available for CORE only	Contact Scott for training
SYBR Green (KAPA/Roche)	$35		NA	per ml		Available for MIT only
96-well PLATES	$6		NA	per plate		Available for MIT only

prices effective 7.1.2014 .

OTHER LAB SERVICES

OTHER LAB SERVICES	CORE LAB	MIT	Non-MIT	unit	SIGN UP	Notes
PIPPIN PREP
Gel Isolation	$50	$55	$75	per gel (5 lanes)	email biomicro
Training	$25	$30	$50	per session	email biomicro	Contact Shmulik for training.
PLATE READER
Varioskan	$12	$13.2	$18	per HOUR	CALENDER	Quick scans (those requiring less then 20 minutes) are free of charge but may not be scheduled on the calender. Users who have large experiments have priority. Contact Shmulik for training
SONICATOR
Covaris S2	$10	$11	$15	per HOUR	CALENDER
Covaris Training	$25	$28	$50	per session		Contact Shmulik for training
ROBOTICS
Tecan EVO 150	$60	$66	$150	per 2h block - based on scheduled time.	CALENDER
Tecan EVO 150 Practice	$10	$11	$150	per 1h block - based on scheduled time. Must indicate practice on the calendar.	CALENDER
non-filter tips	$6.00			per box		** Please let us know if there is additional plasticware you would like us to stock.
Filter tips	$8.00			per box
Training	$100	$110	$150	per session	Contact Stuart
NANODROP
Nanodrop	--				Walk up service	* no charge

prices effective 7.1.2014

COMPUTATIONAL SERVICES

COMPUTATIONAL SERVICES	CORE LAB	Other MIT / Non-MIT	unit	Notes
Informatics Support	$70	$90 / $135	per hour	Limited support for non-MIT users
Informatics Share	$960	N/A	per 0.1FTE	MIT only. Priority access to informatics.
Data storage (1 year)	$280	$400 / N/A	per TB