BioMicroCenter:PricingFY2015: Difference between revisions
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(New page: {{BioMicroCenter}} == ILLUMINA SEQUENCING == DRAFT - NOT FOR USE === SEQUENCING === Please note that samples submitted from [[BioMicroCenter:CoreDeps|'''COR...) |
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Please note that samples submitted from [[BioMicroCenter:CoreDeps|'''CORE LAB''']]s will be given priority on the HiSeq. | Please note that samples submitted from [[BioMicroCenter:CoreDeps|'''CORE LAB''']]s will be given priority on the HiSeq. | ||
{| border=1 | {| border=1 | ||
! ILLUMINA SEQUENCING | ! ILLUMINA SEQUENCING | ||
!width= | !width=80| [[BioMicroCenter:CoreDeps|CORE LAB]] | ||
!width= | !width=80| MIT | ||
!width= | !width=80| [[BioMicroCenter:FAQ#NON_MIT_USERS|Non-MIT]] | ||
!width=80| unit | |||
! Notes | ! Notes | ||
|- | |- | ||
| colspan=" | | colspan="6" align=center |<BR>'''HiSEQ 2000''' sequencing (minimum 150m reads/lane*. Average ~160-240m reads/lane) | ||
|- | |- | ||
| Single End (40nt) | | Single End (40nt) | ||
|align="right"| $1,085 | |align="right"| $1,085 | ||
|align="right"| $1,195 | |||
|align="right"| $1,628 | |align="right"| $1,628 | ||
|align="center"| per lane | |align="center"| per lane | ||
Line 24: | Line 26: | ||
| Paired End (40+40nt) | | Paired End (40+40nt) | ||
|align="right"| $1,625 | |align="right"| $1,625 | ||
|align="right"| $1,788 | |||
|align="right"| $2,438 | |align="right"| $2,438 | ||
|align="center"| per lane | |align="center"| per lane | ||
Line 30: | Line 33: | ||
| Add'l 40nt | | Add'l 40nt | ||
|align="right"| $300 | |align="right"| $300 | ||
|align="right"| $330 | |||
|align="right"| $450 | |align="right"| $450 | ||
|align="center"| per lane | |align="center"| per lane | ||
| | | | ||
|- | |- | ||
| colspan=" | | colspan="6" align=center |<BR>'''MiSeq v2''' sequencing (minimum 8m reads/lane. maximum ~15m reads/lane) | ||
|- | |- | ||
| 70nt Run | | 70nt Run | ||
|align="right"| $1, | |align="right"| $1,150 | ||
|align="right"| $1, | |align="right"| $1,265 | ||
|align="right"| $1,725 | |||
|align="center"| per lane | |align="center"| per lane | ||
| Includes quality control (RT-PCR and BioAnalyzer), sequencing, genome alignment and data storage of BAM files for 1 year | | Includes quality control (RT-PCR and BioAnalyzer), sequencing, genome alignment and data storage of BAM files for 1 year | ||
|- | |- | ||
| 300nt Run (PE or SE) | | 300nt Run (PE or SE) | ||
|align="right"| $1, | |align="right"| $1,350 | ||
|align="right"| $1, | |align="right"| $1,485 | ||
|align="right"| $2,025 | |||
|align="center"| per lane | |align="center"| per lane | ||
| Includes quality control (RT-PCR and BioAnalyzer), sequencing, genome alignment and data storage of BAM files for 1 year | | Includes quality control (RT-PCR and BioAnalyzer), sequencing, genome alignment and data storage of BAM files for 1 year | ||
|- | |- | ||
| 500nt Run (PE or SE) | | 500nt Run (PE or SE) | ||
|align="right"| $1, | |align="right"| $1,600 | ||
|align="right"| $2, | |align="right"| $1,760 | ||
|align="right"| $2,400 | |||
|align="center"| per lane | |align="center"| per lane | ||
| Includes quality control (RT-PCR and BioAnalyzer), sequencing, genome alignment and data storage of BAM files for 1 year | | Includes quality control (RT-PCR and BioAnalyzer), sequencing, genome alignment and data storage of BAM files for 1 year | ||
|- | |- | ||
| colspan=" | | colspan="6" align=center |<BR>'''MiSeq v3''' sequencing (minimum 15m reads/lane. Expected ~25m reads/lane) | ||
|- | |- | ||
| 600nt Run (PE or SE) | | 600nt Run (PE or SE) | ||
|align="right"| $ | |align="right"| $2,050 | ||
|align="right"| $2, | |align="right"| $2,255 | ||
|align="right"| $3,075 | |||
|align="center"| per lane | |align="center"| per lane | ||
| Includes quality control (RT-PCR and BioAnalyzer), sequencing, genome alignment and data storage of BAM files for 1 year | | Includes quality control (RT-PCR and BioAnalyzer), sequencing, genome alignment and data storage of BAM files for 1 year | ||
|- | |- | ||
| colspan=" | | colspan="6" align=center |<BR>'''GAII / GAIIx''' sequencing (Average ~25-40m reads/lane) | ||
|- | |- | ||
| | | colspan="6" align=center |GAII Services are being phased out in FY2015. Requests that must be processed on the GAII will be priced per experiment. | ||
|- | |- | ||
| | | colspan="6" align=center |<BR>'''Additional Services''' | ||
|- | |- | ||
| colspan=" | | colspan="6" align=center |''These services apply to samples submitted for all platforms.'' | ||
|- | |- | ||
| Sample Multiplexing | | Sample Multiplexing | ||
|align="right"| $50 | |align="right"| $50 | ||
|align="right"| $55 | |||
|align="right"| $75 | |align="right"| $75 | ||
|align="center"| per 10 samples | |align="center"| per 10 samples | ||
| Combined over the whole order. Only applies to samples combined by BMC technicians. | | Combined over the whole order. Only applies to samples combined by BMC technicians. | ||
|- | |- | ||
| Add'l Repriming | | Add'l Repriming (HiSeq only) | ||
|align="right"| $200 | |align="right"| $200 | ||
|align="right"| $220 | |||
|align="right"| $300 | |align="right"| $300 | ||
|align="center"| per flow cell | |align="center"| per flow cell | ||
Line 126: | Line 94: | ||
| Quality Control Only | | Quality Control Only | ||
|align="right"| $25 | |align="right"| $25 | ||
|align="right"| $28 | |||
|align="right"| $38 | |align="right"| $38 | ||
|align="center"| per sample | |align="center"| per sample | ||
| Includes RT-PCR and BioAnalyzer | | Includes RT-PCR and BioAnalyzer | ||
|} | |||
<BR> | |||
<BR><BR> | |||
Please note that samples submitted from [[BioMicroCenter:CoreDeps|'''CORE LAB''']]s will be given priority on all equipment. The BioMicro Center reserves the right to reject samples for any reason.''<BR> | |||
=== SAMPLE PREPARATION === | === SAMPLE PREPARATION === | ||
{| border=1 | {| border=1 | ||
! ILLUMINA Sample Preparation | ! ILLUMINA Sample Preparation | ||
!width= | !width=80| [[BioMicroCenter:CoreDeps|CORE LAB]] | ||
!width= | !width=80| MIT | ||
!width= | !width=80| [[BioMicroCenter:FAQ#NON_MIT_USERS|Non-MIT]] | ||
!width=80| unit | |||
! Notes | ! Notes | ||
|- | |- | ||
| colspan=" | | colspan="6" | '''STARTING WITH DNA''' | ||
|- | |- | ||
| Full Service Sheared DNA (SPRIWORKS) | | Full Service Sheared DNA (SPRIWORKS) | ||
|align="right"| $150 | |align="right"| $150 | ||
|align="right"| $165 | |||
|align="right"| $225 | |align="right"| $225 | ||
|align="center"| per sample | |align="center"| per sample | ||
Line 155: | Line 125: | ||
| ''SPRI-TE use only'' | | ''SPRI-TE use only'' | ||
|align="right"| $100 | |align="right"| $100 | ||
|align="right"| $110 | |||
|align="right"| N/A | |align="right"| N/A | ||
|align="center"| per sample | |align="center"| per sample | ||
| User must provide all primers. Samples will be returned for amplification. | | User must provide all primers. Samples will be returned for amplification. | ||
|- | |- | ||
| | | Intact Genomic DNA (NEXTERA) - 50ng input | ||
|align="right"| $175 | |||
|align="right"| $193 | |||
|align="right"| $263 | |||
|align="center"| per sample | |||
| Includes clean up, tagmentation, barcoding, enrichment, and quality control (RT-PCR and BioAnalyzer). | |||
|- | |||
| Low Input Intact Genomic DNA (NEXTERA-XT) - 1ng input | |||
|align="right"| $100 | |||
|align="right"| $110 | |||
|align="right"| $150 | |align="right"| $150 | ||
|align="center"| per sample | |align="center"| per sample | ||
| Includes | | Includes tagmentation, barcoding, enrichment, and quality control (RT-PCR and BioAnalyzer). | ||
|- | |||
| 16S Amplicon | |||
|align="right"| $1,200 | |||
|align="right"| $1,320 | |||
|align="right"| $1,800 | |||
|align="center"| per 48 sample | |||
| Includes normalization, enrichment, pooling and quality control (RT-PCR and BioAnalyzer). | |||
|- | |||
| Other Amplicon | |||
|align="right"| $960 | |||
|align="right"| $1,056 | |||
|align="right"| $1,440 | |||
|align="center"| per 48 sample | |||
| Includes normalization, enrichment, pooling and quality control (RT-PCR and BioAnalyzer). <BR> Samples *must* be submitted amplified with appropriate linkers. | |||
|- | |- | ||
| colspan=" | | colspan="6" | '''STARTING WITH RNA''' | ||
|- | |- | ||
| Illumina TruSeq | | Illumina TruSeq | ||
|align="right"| $300 | |align="right"| $300 | ||
|align="right"| $330 | |||
|align="right"| $450 | |align="right"| $450 | ||
|align="center"| per sample | |align="center"| per sample | ||
Line 175: | Line 169: | ||
|''+strand specific'' | |''+strand specific'' | ||
|align="right"| $30 | |align="right"| $30 | ||
|align="right"| $33 | |||
|align="right"| $45 | |align="right"| $45 | ||
|align="center"| per sample | |align="center"| per sample | ||
Line 181: | Line 176: | ||
| Epicenter Ribozero<br>''no polyA pulldown'' | | Epicenter Ribozero<br>''no polyA pulldown'' | ||
|align="right"| $350 | |align="right"| $350 | ||
|align="right"| $385 | |||
|align="right"| $525 | |align="right"| $525 | ||
|align="center"| per sample | |align="center"| per sample | ||
Line 187: | Line 183: | ||
| Low Input (Clontech) | | Low Input (Clontech) | ||
|align="right"| $400 | |align="right"| $400 | ||
|align="right"| $440 | |||
|align="right"| $600 | |align="right"| $600 | ||
|align="center"| per sample | |align="center"| per sample | ||
Line 192: | Line 189: | ||
|- | |- | ||
| Low Input (NuGen) | | Low Input (NuGen) | ||
|align="right"| $ | |align="right"| $475 | ||
|align="right"| $ | |align="right"| $523 | ||
|align="right"| $713 | |||
|align="center"| per sample | |align="center"| per sample | ||
| Can work on degraded RNA. | | Can work on degraded RNA. | ||
|- | |- | ||
| Tight Size Cut | | Tight Size Cut | ||
|align="right"| $ | |align="right"| $100 | ||
|align="right"| $ | |align="right"| $110 | ||
|align="right"| $150 | |||
|align="center"| per pool | |align="center"| per pool | ||
| +/- 40bp using PippinPrep | | +/- 40bp using PippinPrep | ||
Line 205: | Line 204: | ||
| Small RNA | | Small RNA | ||
| align="right"|$275 | | align="right"|$275 | ||
| align="right"|$303 | |||
| align="right"|$413 | | align="right"|$413 | ||
| align="center"|per sample | | align="center"|per sample | ||
| Uses NEB small RNA kit | | Uses NEB small RNA kit | ||
|- | |- | ||
| colspan=" | | colspan="6" | '''STARTING WITH CHROMATIN''' Note: User must supply antibodies. | ||
|- | |- | ||
| Full Service ChIP-seq | | Full Service ChIP-seq | ||
|align="right"| $210 | |align="right"| $210 | ||
|align="right"| $231 | |||
|align="right"| $315 | |align="right"| $315 | ||
|align="center"| per sample | |align="center"| per sample | ||
Line 219: | Line 220: | ||
| ''IP-Star Only'' | | ''IP-Star Only'' | ||
|align="right"| $300 | |align="right"| $300 | ||
|align="right"| $330 | |||
|align="right"| $450 | |align="right"| $450 | ||
|align="center"| per batch (1-8 samples) | |align="center"| per batch (1-8 samples) | ||
Line 224: | Line 226: | ||
|- | |- | ||
|} | |} | ||
. | . | ||
<BR> | <BR> | ||
Line 248: | Line 248: | ||
|- | |- | ||
| Eukaryote (3' or Exon: >20ng) | | Eukaryote (3' or Exon: >20ng) | ||
|align="right"| $ | |align="right"| $330 | ||
|align="right"| $ | |align="right"| $410 | ||
|align="right"| $ | |align="right"| $600 | ||
|align="center"| per sample | |align="center"| per sample | ||
| Using NuGEN sample labeling kit. | | Using NuGEN sample labeling kit. | ||
|- | |- | ||
| Eukaryote (<20ng) | | Eukaryote (<20ng) | ||
|align="right"| $ | |align="right"| $430 | ||
|align="right"| $ | |align="right"| $530 | ||
|align="right"| $ | |align="right"| $780 | ||
|align="center"| per sample | |align="center"| per sample | ||
| Using NuGEN sample labeling kit. | | Using NuGEN sample labeling kit. | ||
|- | |- | ||
| Prokaryote | | Prokaryote | ||
|align="right"| $ | |align="right"| $400 | ||
|align="right"| $ | |align="right"| $480 | ||
|align="right"| $ | |align="right"| $700 | ||
|align="center"| per sample | |align="center"| per sample | ||
| Using Ambion sample labeling kit. | | Using Ambion sample labeling kit. | ||
Line 270: | Line 270: | ||
| colspan="6" | '''AGILENT PROCESSING''' | | colspan="6" | '''AGILENT PROCESSING''' | ||
|- | |- | ||
| Expression - ONE COLOR | | Expression - ONE COLOR | ||
|align="right"| $ | |align="right"| $225 | ||
|align="right"| $ | |align="right"| $275 | ||
|align="right"| $ | |align="right"| $400 | ||
|align="center"| per array | |align="center"| per array | ||
| | | | ||
|- | |- | ||
| Expression - TWO COLOR | | Expression - TWO COLOR | ||
|align="right"| $ | |align="right"| $325 | ||
|align="right"| $ | |align="right"| $375 | ||
|align="right"| $ | |align="right"| $550 | ||
|align="center"| per array | |align="center"| per array | ||
| Includes both Cy5 and Cy3 | | Includes both Cy5 and Cy3 | ||
|- | |- | ||
| Scanning Only | | Scanning Only | ||
Line 306: | Line 292: | ||
|- | |- | ||
|} | |} | ||
prices effective 7.1. | prices effective 7.1.2014 | ||
<br> | <br> | ||
Line 320: | Line 306: | ||
{| border=1 | {| border=1 | ||
! AGILENT BIOANALYZER / ADVANCED ANALYTICAL-FRAGMENT ANALYZER | ! AGILENT BIOANALYZER / ADVANCED ANALYTICAL-FRAGMENT ANALYZER | ||
!width= | !width=80| [[BioMicroCenter:CoreDeps|CORE LAB]] | ||
!width= | !width=80| MIT | ||
!width= | !width=80| [[BioMicroCenter:FAQ#NON_MIT_USERS|Non-MIT]] | ||
!width=80| unit | |||
! Notes | ! Notes | ||
|- | |- | ||
| RNA / DNA on Advanced Analytical | | RNA / DNA on Advanced Analytical | ||
|align="right"| $8 | |||
|align="right"| $8 | |align="right"| $8 | ||
|align="right"| $12 | |align="right"| $12 | ||
Line 333: | Line 321: | ||
| AA - Preloaded plate | | AA - Preloaded plate | ||
|align="right"| $30 | |align="right"| $30 | ||
|align="right"| | |align="right"| $33 | ||
|align="right"| $45 | |||
|align="center"| per row (12) | |align="center"| per row (12) | ||
| 2ul per sample in each well using plates from BMC | | 2ul per sample in each well using plates from BMC | ||
|- | |- | ||
| All DNA & RNA on BIOANALYZER | | All DNA & RNA on BIOANALYZER | ||
|align="right"| $15 | |||
|align="right"| $15 | |align="right"| $15 | ||
|align="right"| $25 | |align="right"| $25 | ||
Line 345: | Line 335: | ||
| High Sensitivity protein | | High Sensitivity protein | ||
|align="right"| $40 | |align="right"| $40 | ||
|align="right"| $45 | |||
|align="right"| $60 | |align="right"| $60 | ||
|align="center"| per sample | |align="center"| per sample | ||
| detection of proteins at < 30ng/ul. | | detection of proteins at < 30ng/ul. | ||
|- | |||
| Self run chips | |||
|align="right"| $55-90 | |||
|align="right"| request | |||
|align="right"| NA | |||
|align="center"| per chip | |||
| Price varies by flavor of Chip. | |||
|- | |- | ||
|} | |} | ||
prices effective 7.1. | prices effective 7.1.2014 | ||
<br>. | <br>. | ||
Line 357: | Line 355: | ||
{| border=1 | {| border=1 | ||
! OTHER LAB SERVICES | ! OTHER LAB SERVICES | ||
!width= | !width=80| [[BioMicroCenter:CoreDeps|CORE LAB MIT]] | ||
!width= | !width=80| MIT | ||
!width= | !width=80| [[BioMicroCenter:FAQ#NON_MIT_USERS|Non-MIT]] | ||
!width=80| unit | |||
! SIGN UP | ! SIGN UP | ||
! Notes | ! Notes | ||
|- | |- | ||
| colspan=" | | colspan="7" align="center"| '''FLUIDIGM''' | ||
|- | |- | ||
| Fluidigm BioMark | | Fluidigm BioMark | ||
|align="right"| $150 | |align="right"| $150 | ||
|align="right"| $165 | |||
|align="right"| $225 | |align="right"| $225 | ||
|align="center"| per use | |align="center"| per use | ||
Line 373: | Line 373: | ||
|- | |- | ||
| 48.48 Dynamic Array Chip | | 48.48 Dynamic Array Chip | ||
|align="right"| $250 | |colspan="2" align="right"| $250 | ||
|align="right"| N/A | |align="right"| N/A | ||
|align="center"| per cassette | |align="center"| per cassette | ||
Line 380: | Line 380: | ||
|- | |- | ||
| 96.96 Dynamic Array Chip | | 96.96 Dynamic Array Chip | ||
|align="right"| $700 | |colspan="2" align="right"| $700 | ||
|align="right"| N/A | |align="right"| N/A | ||
|align="center"| per cassette | |align="center"| per cassette | ||
Line 387: | Line 387: | ||
|- | |- | ||
| WAFERGEN SMART CHIP | | WAFERGEN SMART CHIP | ||
|align="right"| $150 | |colspan="2" align="right"| $150 | ||
|align="right"| N/A | |align="right"| N/A | ||
|align="center"| per chip | |align="center"| per chip | ||
Line 395: | Line 395: | ||
| WAFERGEN TRAINING | | WAFERGEN TRAINING | ||
|align="right"| $100 | |align="right"| $100 | ||
|align="right"| $110 | |||
|align="right"| N/A | |align="right"| N/A | ||
|align="center"| per chip | |align="center"| per chip | ||
| Mandatory | | Mandatory | ||
| Contact [[BioMicroCenter:People|Michael]] | | Contact [[BioMicroCenter:People|Michael]] | ||
|- | colspan=" | |- | ||
| colspan="7" align="center"| '''RT-PCR''' | |||
|- | |- | ||
| Roche LightCycler 480 | | Roche LightCycler 480 | ||
|align="right"| $ | |align="right"| $15 | ||
|align="right"| $20 | |||
|align="right"| NA | |align="right"| NA | ||
|align="center"| per plate | |align="center"| per plate | ||
Line 409: | Line 412: | ||
|- | |- | ||
| SYBR Green (KAPA/Roche) | | SYBR Green (KAPA/Roche) | ||
|align="right"| $35 | |colspan="2" align="right"| $35 | ||
|align="right"| NA | |align="right"| NA | ||
|align="center"| per ml | |align="center"| per ml | ||
Line 416: | Line 419: | ||
|- | |- | ||
| 96-well PLATES | | 96-well PLATES | ||
|align="right"| $6 | |colspan="2" align="right"| $6 | ||
|align="right"| NA | |align="right"| NA | ||
|align="center"| per plate | |align="center"| per plate | ||
Line 423: | Line 426: | ||
|- | |- | ||
|} | |} | ||
prices effective 7.1. | prices effective 7.1.2014 | ||
. | . | ||
<br> | <br> | ||
Line 431: | Line 434: | ||
{| border=1 | {| border=1 | ||
! OTHER LAB SERVICES | ! OTHER LAB SERVICES | ||
!width= | !width=80| [[BioMicroCenter:CoreDeps|CORE LAB]] | ||
!width= | !width=80| MIT | ||
! | !width=80| [[BioMicroCenter:FAQ#NON_MIT_USERS|Non-MIT]] | ||
! unit | |||
! SIGN UP | ! SIGN UP | ||
! Notes | ! Notes | ||
|- | |- | ||
| colspan=" | | colspan="7" align="center"| '''PIPPIN PREP''' | ||
|- | |- | ||
|Gel Isolation | |Gel Isolation | ||
|align="right"| $50 | |align="right"| $50 | ||
|align="right"| $55 | |||
|align="right"| $75 | |align="right"| $75 | ||
|align="center"| per gel (5 lanes) | |align="center"| per gel (5 lanes) | ||
| | | email biomicro | ||
| | | | ||
|- | |- | ||
|Training | |Training | ||
|align="right"| $25 | |align="right"| $25 | ||
|align="right"| $30 | |||
|align="right"| $50 | |align="right"| $50 | ||
|align="center"| per session | |align="center"| per session | ||
| | | email biomicro | ||
| Contact [[BioMicroCenter:People|Shmulik]] for training. | | Contact [[BioMicroCenter:People|Shmulik]] for training. | ||
|- | |- | ||
| colspan=" | | colspan="7" align="center"| '''PLATE READER''' | ||
|- | |- | ||
| Varioskan | | Varioskan | ||
|align="right"| $12 | |||
|align="right"| $13.2 | |||
|align="right"| $18 | |||
|align="center"| per HOUR | |||
| [[BioMicroCenter:Forms#Calenders|CALENDER]] | |||
| Quick scans (those requiring less then ''20'' minutes) are free of charge but may not be scheduled on the calender. Users who have large experiments have priority. Contact [[BioMicroCenter:People|Shmulik]] for training | |||
|- | |||
| colspan="7" align="center"| '''SONICATOR''' | |||
|- | |||
| Covaris S2 | |||
|align="right"| $10 | |align="right"| $10 | ||
|align="right"| $11 | |||
|align="right"| $15 | |align="right"| $15 | ||
|align="center"| per HOUR | |||
| [[BioMicroCenter:Forms#Calenders|CALENDER]] | |||
| | |||
|- | |||
| Covaris Training | |||
|align="right"| $25 | |||
|align="right"| $28 | |||
|align="right"| $50 | |||
|align="center"| per session | |align="center"| per session | ||
| | | | ||
| | | Contact [[BioMicroCenter:People|Shmulik]] for training | ||
|- | |- | ||
| colspan=" | | colspan="7" align="center"| '''ROBOTICS''' | ||
|- | |- | ||
| Tecan EVO 150 | | Tecan EVO 150 | ||
|align="right"| $60 | |align="right"| $60 | ||
|align="right"| $66 | |||
|align="right"| $150 | |align="right"| $150 | ||
|align="center"| per 2h block - based on scheduled time. | |align="center"| per 2h block - based on scheduled time. | ||
| [[BioMicroCenter:Forms#Calenders|CALENDER]] | |||
| | |||
|- | |||
| Tecan EVO 150 Practice | |||
|align="right"| $10 | |||
|align="right"| $11 | |||
|align="right"| $150 | |||
|align="center"| per 1h block - based on scheduled time.<BR> Must indicate practice on the calendar. | |||
| [[BioMicroCenter:Forms#Calenders|CALENDER]] | | [[BioMicroCenter:Forms#Calenders|CALENDER]] | ||
| | | | ||
|- | |- | ||
|non-filter tips | |non-filter tips | ||
| | |colspan="3" align="center"| $6.00 | ||
|align="center"| per box | |align="center"| per box | ||
| | | | ||
Line 479: | Line 512: | ||
|- | |- | ||
|Filter tips | |Filter tips | ||
| | |colspan="3" align="center"| $8.00 | ||
|align="center"| per box | |align="center"| per box | ||
| | | | ||
Line 487: | Line 519: | ||
| Training | | Training | ||
|align="right"| $100 | |align="right"| $100 | ||
|align="right"| $110 | |||
|align="right"| $150 | |align="right"| $150 | ||
|align="center"| per session | |align="center"| per session | ||
Line 492: | Line 525: | ||
| | | | ||
|- | |- | ||
| colspan=" | | colspan="7" align="center"| '''NANODROP''' | ||
|- | |- | ||
| Nanodrop | | Nanodrop | ||
| | |colspan="3" align="center"| -- | ||
|align="center"| | |align="center"| | ||
| Walk up service | | Walk up service | ||
Line 502: | Line 534: | ||
|- | |- | ||
|} | |} | ||
prices effective 7.1. | prices effective 7.1.2014 | ||
<br><br> | <br><br> | ||
Line 527: | Line 559: | ||
| Data storage (1 year) | | Data storage (1 year) | ||
|align="right"| $280 | |align="right"| $280 | ||
|align="right"| N/A | |align="right"| $400 / N/A | ||
|align="center"| per TB | |align="center"| per TB | ||
| | | | ||
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Latest revision as of 10:22, 27 April 2014
HOME -- | SEQUENCING -- | LIBRARY PREP -- | HIGH-THROUGHPUT -- | COMPUTING -- | OTHER TECHNOLOGY |
ILLUMINA SEQUENCING
DRAFT - NOT FOR USE
SEQUENCING
Please note that samples submitted from CORE LABs will be given priority on the HiSeq.
ILLUMINA SEQUENCING | CORE LAB | MIT | Non-MIT | unit | Notes |
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HiSEQ 2000 sequencing (minimum 150m reads/lane*. Average ~160-240m reads/lane) | |||||
Single End (40nt) | $1,085 | $1,195 | $1,628 | per lane | Includes quality control (RT-PCR and BioAnalyzer), sequencing, genome alignment and data storage of BAM files for 1y. |
Paired End (40+40nt) | $1,625 | $1,788 | $2,438 | per lane | |
Add'l 40nt | $300 | $330 | $450 | per lane | |
MiSeq v2 sequencing (minimum 8m reads/lane. maximum ~15m reads/lane) | |||||
70nt Run | $1,150 | $1,265 | $1,725 | per lane | Includes quality control (RT-PCR and BioAnalyzer), sequencing, genome alignment and data storage of BAM files for 1 year |
300nt Run (PE or SE) | $1,350 | $1,485 | $2,025 | per lane | Includes quality control (RT-PCR and BioAnalyzer), sequencing, genome alignment and data storage of BAM files for 1 year |
500nt Run (PE or SE) | $1,600 | $1,760 | $2,400 | per lane | Includes quality control (RT-PCR and BioAnalyzer), sequencing, genome alignment and data storage of BAM files for 1 year |
MiSeq v3 sequencing (minimum 15m reads/lane. Expected ~25m reads/lane) | |||||
600nt Run (PE or SE) | $2,050 | $2,255 | $3,075 | per lane | Includes quality control (RT-PCR and BioAnalyzer), sequencing, genome alignment and data storage of BAM files for 1 year |
GAII / GAIIx sequencing (Average ~25-40m reads/lane) | |||||
GAII Services are being phased out in FY2015. Requests that must be processed on the GAII will be priced per experiment. | |||||
Additional Services | |||||
These services apply to samples submitted for all platforms. | |||||
Sample Multiplexing | $50 | $55 | $75 | per 10 samples | Combined over the whole order. Only applies to samples combined by BMC technicians. |
Add'l Repriming (HiSeq only) | $200 | $220 | $300 | per flow cell | Uses 6-8nt. Cost is divided by number of lanes requiring repriming. Repriming is required for standard Illumina multiplexing. |
Quality Control Only | $25 | $28 | $38 | per sample | Includes RT-PCR and BioAnalyzer |
Please note that samples submitted from CORE LABs will be given priority on all equipment. The BioMicro Center reserves the right to reject samples for any reason.
SAMPLE PREPARATION
ILLUMINA Sample Preparation | CORE LAB | MIT | Non-MIT | unit | Notes |
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STARTING WITH DNA | |||||
Full Service Sheared DNA (SPRIWORKS) | $150 | $165 | $225 | per sample | Includes adapter ligation, size selection, barcoding, enrichment, and quality control (RT-PCR and BioAnalyzer). |
SPRI-TE use only | $100 | $110 | N/A | per sample | User must provide all primers. Samples will be returned for amplification. |
Intact Genomic DNA (NEXTERA) - 50ng input | $175 | $193 | $263 | per sample | Includes clean up, tagmentation, barcoding, enrichment, and quality control (RT-PCR and BioAnalyzer). |
Low Input Intact Genomic DNA (NEXTERA-XT) - 1ng input | $100 | $110 | $150 | per sample | Includes tagmentation, barcoding, enrichment, and quality control (RT-PCR and BioAnalyzer). |
16S Amplicon | $1,200 | $1,320 | $1,800 | per 48 sample | Includes normalization, enrichment, pooling and quality control (RT-PCR and BioAnalyzer). |
Other Amplicon | $960 | $1,056 | $1,440 | per 48 sample | Includes normalization, enrichment, pooling and quality control (RT-PCR and BioAnalyzer). Samples *must* be submitted amplified with appropriate linkers. |
STARTING WITH RNA | |||||
Illumina TruSeq | $300 | $330 | $450 | per sample | Preformed using TruSeq AND SPRIworks system. Includes purifying mRNA, generation of fragmented dsDNA, adaptor ligation, size selection, barcoding, enrichment, and quality control (RT-PCR and BioAnalyzer). |
+strand specific | $30 | $33 | $45 | per sample | Requires 1ug of RNA. Uses the dUTP method. |
Epicenter Ribozero no polyA pulldown |
$350 | $385 | $525 | per sample | Requires 1ug of RNA. Based on Illumina Truseq chemistry |
Low Input (Clontech) | $400 | $440 | $600 | per sample | Can work on single cells |
Low Input (NuGen) | $475 | $523 | $713 | per sample | Can work on degraded RNA. |
Tight Size Cut | $100 | $110 | $150 | per pool | +/- 40bp using PippinPrep |
Small RNA | $275 | $303 | $413 | per sample | Uses NEB small RNA kit |
STARTING WITH CHROMATIN Note: User must supply antibodies. | |||||
Full Service ChIP-seq | $210 | $231 | $315 | per sample | Preformed using IP-Star AND SPRIworks system. Includes ChIP, library contruction and barcoding. |
IP-Star Only | $300 | $330 | $450 | per batch (1-8 samples) | Preformed using IP-Star |
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MICROARRAYS
Microarray processing prices do not include the cost of the array. In order to be sure we have arrays on hand, we encourage users to contact us several days in advance of submitting samples.
MICROARRAYS | CORE LAB | MIT | Non-MIT | unit | Notes |
---|---|---|---|---|---|
AFFYMETRIX PROCESSING | |||||
Eukaryote (3' or Exon: >20ng) | $330 | $410 | $600 | per sample | Using NuGEN sample labeling kit. |
Eukaryote (<20ng) | $430 | $530 | $780 | per sample | Using NuGEN sample labeling kit. |
Prokaryote | $400 | $480 | $700 | per sample | Using Ambion sample labeling kit. |
AGILENT PROCESSING | |||||
Expression - ONE COLOR | $225 | $275 | $400 | per array | |
Expression - TWO COLOR | $325 | $375 | $550 | per array | Includes both Cy5 and Cy3 |
Scanning Only | $50 | $50 | N/A | per slide | Price per glass slide. |
prices effective 7.1.2014
Common Affymetrix Arrays* (some may be in stock):
$175 - Prokaryote/Small Eukaryote E.coli Genome $250 - Eukaryote 3' (low density) Human U133A 2.0 / Mouse 430A 2.0 $400 - Eukaryote Exon or 3' (high density) Human U133+ 2.0 / Mouse 430 2.0 / Human or Mouse Exon 1.0 ST Full Price List: Affymetrix Prices
- available at cost to academic labs only. shipping fees may be added. Non-academic labs should order their own arrays and have them shipped to the BioMicro Center.
AGILENT BIOANALYZER
AGILENT BIOANALYZER / ADVANCED ANALYTICAL-FRAGMENT ANALYZER | CORE LAB | MIT | Non-MIT | unit | Notes |
---|---|---|---|---|---|
RNA / DNA on Advanced Analytical | $8 | $8 | $12 | per sample | |
AA - Preloaded plate | $30 | $33 | $45 | per row (12) | 2ul per sample in each well using plates from BMC |
All DNA & RNA on BIOANALYZER | $15 | $15 | $25 | per sample | |
High Sensitivity protein | $40 | $45 | $60 | per sample | detection of proteins at < 30ng/ul. |
Self run chips | $55-90 | request | NA | per chip | Price varies by flavor of Chip. |
prices effective 7.1.2014
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REAL TIME PCR
OTHER LAB SERVICES | CORE LAB MIT | MIT | Non-MIT | unit | SIGN UP | Notes |
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FLUIDIGM | ||||||
Fluidigm BioMark | $150 | $165 | $225 | per use | CALENDER | Contact Shmulik |
48.48 Dynamic Array Chip | $250 | N/A | per cassette | Cassettes are available in the BMC. | ||
96.96 Dynamic Array Chip | $700 | N/A | per cassette | Cassettes are available in the BMC. | ||
WAFERGEN SMART CHIP | $150 | N/A | per chip | Chips available from BMC | Contact Michael | |
WAFERGEN TRAINING | $100 | $110 | N/A | per chip | Mandatory | Contact Michael |
RT-PCR | ||||||
Roche LightCycler 480 | $15 | $20 | NA | per plate | CALENDER *available for CORE only | Contact Scott for training |
SYBR Green (KAPA/Roche) | $35 | NA | per ml | Available for MIT only | ||
96-well PLATES | $6 | NA | per plate | Available for MIT only |
prices effective 7.1.2014
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OTHER LAB SERVICES
OTHER LAB SERVICES | CORE LAB | MIT | Non-MIT | unit | SIGN UP | Notes |
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PIPPIN PREP | ||||||
Gel Isolation | $50 | $55 | $75 | per gel (5 lanes) | email biomicro | |
Training | $25 | $30 | $50 | per session | email biomicro | Contact Shmulik for training. |
PLATE READER | ||||||
Varioskan | $12 | $13.2 | $18 | per HOUR | CALENDER | Quick scans (those requiring less then 20 minutes) are free of charge but may not be scheduled on the calender. Users who have large experiments have priority. Contact Shmulik for training |
SONICATOR | ||||||
Covaris S2 | $10 | $11 | $15 | per HOUR | CALENDER | |
Covaris Training | $25 | $28 | $50 | per session | Contact Shmulik for training | |
ROBOTICS | ||||||
Tecan EVO 150 | $60 | $66 | $150 | per 2h block - based on scheduled time. | CALENDER | |
Tecan EVO 150 Practice | $10 | $11 | $150 | per 1h block - based on scheduled time. Must indicate practice on the calendar. |
CALENDER | |
non-filter tips | $6.00 | per box | ** Please let us know if there is additional plasticware you would like us to stock. | |||
Filter tips | $8.00 | per box | ||||
Training | $100 | $110 | $150 | per session | Contact Stuart | |
NANODROP | ||||||
Nanodrop | -- | Walk up service | * no charge |
prices effective 7.1.2014
COMPUTATIONAL SERVICES
COMPUTATIONAL SERVICES | CORE LAB | Other MIT / Non-MIT | unit | Notes |
---|---|---|---|---|
Informatics Support | $70 | $90 / $135 | per hour | Limited support for non-MIT users |
Informatics Share | $960 | N/A | per 0.1FTE | MIT only. Priority access to informatics. |
Data storage (1 year) | $280 | $400 / N/A | per TB |