BioMicroCenter:Protocols: Difference between revisions
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<B><BIG> Genomic DNA </BIG></B> | <B><BIG> Genomic DNA </BIG></B> | ||
* [http:// | * [http://openwetware.org/images/8/88/Genomic_DNA_Sample_Prep_1003806_RevB.pdf Illumina protocol (full kit protocol)]<br> | ||
* [http:// | * [http://openwetware.org/images/6/6c/Genomic_DNA_Sample_Prep_OligoOnly_1003492_Rev_A.pdf Illumina protocol (oligos only protocol)] - unlike the full kit, this kit provides only the adapters PCR primers. Users wishing to adapting current lab protocols may find this protocol more useful. | ||
<B><BIG> Digital Gene Expression </BIG></B> | <B><BIG> Digital Gene Expression </BIG></B> | ||
* [http:// | * [http://openwetware.org/images/4/47/GEX_NlaIII_Sample_Prep_1004240_RevA.pdf Illumina Protocol (NlaIII, full kit protocol)] | ||
* [http:// | * [http://openwetware.org/images/e/ed/GeneExp_NlaIII_Sample_Prep_OligoOnly_1003652_Rev_A.pdf Illumina Protocol (NlaIII, oligos only protocol)] | ||
* [http:// | * [http://openwetware.org/images/4/46/GEX_DpnII_Sample_Prep_1004241_RevA.pdf Illumina Protocol (DpnII, full kit protocol)] | ||
* [http:// | * [http://openwetware.org/images/1/10/GeneExp_DpnII_Sample_Prep_OligoOnly_1003653_Rev_A.pdf Illumina Protocol (DpnII, olgios only protocol)] | ||
<B><BIG> mRNA Seq </BIG></B> | <B><BIG> mRNA Seq </BIG></B> | ||
* [http:// | * [http://openwetware.org/images/e/e5/MRNA-Seq_SamplePrep_1004898_RevA.pdf Illumina Protocol] | ||
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* [http://www.umassmed.edu/uploadedFiles/nemo/Mellolab_smRNA_cloning_a_la_Tuschl_(071408).doc Tuschl Lab Protocol] | * [http://www.umassmed.edu/uploadedFiles/nemo/Mellolab_smRNA_cloning_a_la_Tuschl_(071408).doc Tuschl Lab Protocol] | ||
* [http:// | * [http://openwetware.org/images/b/bb/GEX_SmallRNA_Sample_Prep_1004239_RevA.pdf Illumina Protocol (full kit protocol)] | ||
* [http:// | * [http://openwetware.org/images/b/bb/SmallRNA_Sample_Prep_OligoOnly_1003651_Rev_A.pdf Illumina Protocol (oligos only protocol)] | ||
* [http://openwetware.org/images/b/bb/SmallRNA_Altv1.5_SamplePrep_15002615_RevA.pdf Illumina Protocol (Alternate v1.5 protocol)] | |||
<B><BIG> Paired End </BIG></B> | |||
* [http://openwetware.org/images/b/bb/Paired-End_Seq_SamplePrep_1005063_RevA.pdf Illumina Protocol] | |||
* [http://openwetware.org/images/b/bb/MultiplexPE_SamplePrep_1005361_RevB.pdf Illumina Protocol (Paired End Multiplexing)] | |||
<B><BIG> Mate Paired Library </BIG></B> | |||
* [http://openwetware.org/images/b/bb/MatePair_2-5kbSamplePrep_1005363_RevB.pdf Illumina Protocol (2-5kb samples)] | |||
Revision as of 14:01, 22 April 2009
| HOME -- | SEQUENCING -- | LIBRARY PREP -- | HIGH-THROUGHPUT -- | COMPUTING -- | OTHER TECHNOLOGY |
BioMicro Center Protocol Testing
ILLUMINA SEQUENCING PROTOCOLS
Cluster Station and Genome Analyzer
ChIP Seq
Genomic DNA
- Illumina protocol (full kit protocol)
- Illumina protocol (oligos only protocol) - unlike the full kit, this kit provides only the adapters PCR primers. Users wishing to adapting current lab protocols may find this protocol more useful.
Digital Gene Expression
mRNA Seq
Small RNA Seq
Paired End
Mate Paired Library
