BioMicroCenter:RTPCR: Difference between revisions

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== PCR ==
== Traditional Real Time PCR ==


[[Image:Primus_multiblock.jpg|right]]
===MWG Thermal Cyclers===
The Primus Multiblock system provides comprehensive state-of-the-art PCR technology.
They are ideal instruments for high throughput PCR and sequencing. The cyclers allow for
exchangeable blocks (384/96 well format), motorized lid, HTR Peltier technology for
fastest heating / cooling rates and optimal results. Patented HPL (High Pressure Lid)
technology for 100% evaporation prevention.




== Real Time PCR ==
=== Light Cycler 480 II Real-time PCR Machines ===
{| align=left
|- valign="top"
| <!-- BMC CORE TABLE -->
{| border="1" cellpadding="5" cellspacing="0" align=right
  ! Instrument || LC480
  |-
  | TYPE || WALKUP - MIT only
  |-
  | UNIT || Per Plate
  |-
  | FORMAT || 96w/394w
  |-
  | SIGNUP || [https://mit.ilabsolutions.com/equipment/show/261680/?tab=schedule ilabs]
  |-
  | NEW USERS ||  New users should request training by emailing biomicro@mit.edu
  |-
  | DONATED BY || Prof Laurie Boyer, Prof Leonard Guarente, Dept of Biology
  |}
| <!-- DESCRIPTION -->
The [https://www.roche-applied-science.com/sis/rtpcr/htc/index.jsp LightCycler 480 Real-Time PCR System] is a fully integrated multiwell-plate based real-time PCR platform for highly accurate qualitative and quantitative detection of nucleic acids. The instrument handles both 96 and 384w plates, rapidly switching between blocks (protocol below). The LC480 can measure Ct and melt curves and can do protein melt curves as well. The instrument handles SYBR and Taqman probes. ROC standards are not required on the instrument, though it can detect them. <BR><BR>


[[Image:Opticon2_system.gif|right]]   
There is a charge per plate to cover the cost of the service contract.<BR><BR>
===MJ Research Real Time PCR Machine===


The MJ Research PCR Machine enables multicolor capability allowing detection of SYBR® Green I or FAM in the first channel, and a range of fluorophores in the second channel — including TET, HEX, VIC, and TAMRA — for a multitude of applications such as RT-qPCR and allelic discrimination. The DNA Engine® thermal cycler offers precision thermal control and a temperature gradient feature permits simultaneous incubation at 12 different temperatures to optimize reactions in a single run. The real-time results allow for plotting of signal intensity vs. cycle number and graphically monitoring the thermal profile during the run. The extraordinary sensitivity permits reliable detection of one initial template copy, while delivering a linear range of up to ten orders of magnitude in starting copy number (with appropriate chemistry). Users are currently charged '''$10''' per plate for the use of this machine. <BR>
96 well plates and SYBR Green can also be purchased from the BioMicro Center for use on the LightCycler480<BR><BR>  
* <B>[[BioMicroCenter:MJO_CAL|Schedule Time on the MJ Opticon]]</B><BR><BR>


[[Image:LC480.jpg|right]]
'''Please Note:''' There are three LightCycler systems (Thing 1, Thing 2, and Thing 3) that have a sign up <B>maximum of four hours per day</b> during business hours (10AM to 5PM), all time slots are available the night before even if it exceeds the maximum.  


=== Light Cycler 480 II Real-time PCR machine ===
The [https://www.roche-applied-science.com/sis/rtpcr/htc/index.jsp LightCycler 480 Real-Time PCR System] is a fully integrated multiwell-plate based real-time PCR platform for highly accurate qualitative and quantitative detection of nucleic acids. Building on the benefits of Roche's capillary-based LightCycler® Systems, it goes one step further in offering enhanced throughput, compatibility with automation equipment and maximum flexibility regarding hard- and software. Providing novel ways to combine speed and accuracy without compromises, the LightCycler® 480 Real-Time PCR System meets the needs of a broad range of applications in research fields such as gene expression studies, discovery and analysis of genetic variation or array data validation.


The Light Cycler will be relocated to 68-316 in mid July 2009 at which time a $10 charge per plate will be implemented to cover the cost of the service contract.
 
[[Image:Block Changing Protocol LightCycler480.txt]]
96 well plates ($5 per plate) and SYBR Green ($40 per mL) can also be supplied on demand for use on the lightCycler480
| <!-- IMAGE -->
 
[[Image:LC480.jpg|right]]
 
|}
 
[http://www.google.com/calendar Schedule Time on the LightCycler 480]
* Username: Building68
* Password: DNARNAprotein

Latest revision as of 20:37, 20 October 2018

HOME -- SEQUENCING -- LIBRARY PREP -- HIGH-THROUGHPUT -- COMPUTING -- OTHER TECHNOLOGY

Traditional Real Time PCR

Light Cycler 480 II Real-time PCR Machines

Instrument LC480
TYPE WALKUP - MIT only
UNIT Per Plate
FORMAT 96w/394w
SIGNUP ilabs
NEW USERS New users should request training by emailing biomicro@mit.edu
DONATED BY Prof Laurie Boyer, Prof Leonard Guarente, Dept of Biology

The LightCycler 480 Real-Time PCR System is a fully integrated multiwell-plate based real-time PCR platform for highly accurate qualitative and quantitative detection of nucleic acids. The instrument handles both 96 and 384w plates, rapidly switching between blocks (protocol below). The LC480 can measure Ct and melt curves and can do protein melt curves as well. The instrument handles SYBR and Taqman probes. ROC standards are not required on the instrument, though it can detect them.

There is a charge per plate to cover the cost of the service contract.

96 well plates and SYBR Green can also be purchased from the BioMicro Center for use on the LightCycler480

Please Note: There are three LightCycler systems (Thing 1, Thing 2, and Thing 3) that have a sign up maximum of four hours per day during business hours (10AM to 5PM), all time slots are available the night before even if it exceeds the maximum.


File:Block Changing Protocol LightCycler480.txt

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