BioMicroCenter:RTPCR for Solexa: Difference between revisions
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Simultaneous test. Samples were tested for RT-PCR and were clustered for sequencing on the same day. Their DNA concentration and the correlation of the concentration as measured by RT-PCR was then compared to their cluster number. All DNA samples had been thought to be at 10nM. <br> | |||
[[Image: | [[Image:|400px]] | ||
Each sample is individually colored and represented as two replicates. |
Revision as of 15:01, 3 February 2009
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Testing of RT-PCR assay to control DNA concentration for Solexa sequencing
Standard curve using serial dilution of PhiX control DNA.
Simultaneous test. Samples were tested for RT-PCR and were clustered for sequencing on the same day. Their DNA concentration and the correlation of the concentration as measured by RT-PCR was then compared to their cluster number. All DNA samples had been thought to be at 10nM.
[[Image:|400px]]
Each sample is individually colored and represented as two replicates.