BioMicroCenter:SPRI-Works: Difference between revisions
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[[Image: BioMicro_SPRIworks.jpg|right| | [[Image: BioMicro_SPRIworks.jpg|right|200px]] | ||
The BMC is currently testing the Beckman SPRIworks library preparation system. This platform is a fully automated library preparation system for Illumina sequencing and supports all Illumina applications except for smallRNA-Seq. It performs all of the traditional Illumina protocol steps listed on the [[BioMicroCenter:Illumina Library Preparation|Illumina Library Preparation page]], except for Enrichment, can prepare up to ten libraries for Illumina sequencing in 5 hours. | The BMC is currently testing the Beckman SPRIworks library preparation system. This platform is a fully automated library preparation system for Illumina sequencing and supports all Illumina applications except for smallRNA-Seq. It performs all of the traditional Illumina protocol steps listed on the [[BioMicroCenter:Illumina Library Preparation|Illumina Library Preparation page]], except for Enrichment, can prepare up to ten libraries for Illumina sequencing in 5 hours. | ||
==How it Works== | ==How it Works== | ||
[[Image:spri-works.jpg|right| | [[Image:spri-works.jpg|right|200px]] | ||
The SPRI-works system consists of three main components: the SPRI-TE Nucleic Acid Extractor, a method card that controls liquid handling, and a cartridge containing all the reagents required to prepare a single library. | The SPRI-works system consists of three main components: the SPRI-TE Nucleic Acid Extractor, a method card that controls liquid handling, and a cartridge containing all the reagents required to prepare a single library. | ||
This platform utilizes DNA binding magnetic beads to purify fragmented DNA and perform size selection, replacing the need for column purification and gel-base size selection. There are two size range options available, 100-400bp or 300-600bp. There is also the choice to perform no size selection | This platform utilizes DNA binding magnetic beads to purify fragmented DNA and perform size selection, replacing the need for column purification and gel-base size selection. There are two size range options available, 100-400bp or 300-600bp. There is also the choice to perform no size selection | ||
Revision as of 12:47, 2 August 2010
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The BMC is currently testing the Beckman SPRIworks library preparation system. This platform is a fully automated library preparation system for Illumina sequencing and supports all Illumina applications except for smallRNA-Seq. It performs all of the traditional Illumina protocol steps listed on the Illumina Library Preparation page, except for Enrichment, can prepare up to ten libraries for Illumina sequencing in 5 hours.
How it Works
The SPRI-works system consists of three main components: the SPRI-TE Nucleic Acid Extractor, a method card that controls liquid handling, and a cartridge containing all the reagents required to prepare a single library.
This platform utilizes DNA binding magnetic beads to purify fragmented DNA and perform size selection, replacing the need for column purification and gel-base size selection. There are two size range options available, 100-400bp or 300-600bp. There is also the choice to perform no size selection
