BioMicroCenter:SPRI-Works: Difference between revisions
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The SPRI-works system consists of three main components: the SPRI-TE Nucleic Acid Extractor, a method card that controls liquid handling, and a cartridge containing all the reagents required to prepare a single library. | The SPRI-works system consists of three main components: the SPRI-TE Nucleic Acid Extractor, a method card that controls liquid handling, and a cartridge containing all the reagents required to prepare a single library. | ||
This platform utilizes DNA binding magnetic beads to purify fragmented DNA and perform size selection, replacing the need for column purification and gel- | This platform utilizes AMPureXP DNA binding magnetic beads to purify fragmented DNA and perform size selection, replacing the need for column purification and gel-based size selection. There are two size range options available, 100-400bp or 300-600bp. There is also the choice to perform no size selection. When complete samples will be ready for PCR Enrichment and clean-up to complete the library prep process. | ||
For more information on the SPRI-Works system please visit www.spriworks.com | For more information on the SPRI-Works system please visit www.spriworks.com | ||
Revision as of 12:59, 2 August 2010
| HOME -- | SEQUENCING -- | LIBRARY PREP -- | HIGH-THROUGHPUT -- | COMPUTING -- | OTHER TECHNOLOGY |
The BMC is currently testing the Beckman SPRIworks library preparation system. This platform is a fully automated library preparation system for Illumina sequencing and supports all Illumina applications except for smallRNA-Seq. It performs all of the traditional Illumina protocol steps listed on the Illumina Library Preparation page, except for Enrichment, can prepare up to ten libraries for Illumina sequencing in 5 hours.
How it Works
The SPRI-works system consists of three main components: the SPRI-TE Nucleic Acid Extractor, a method card that controls liquid handling, and a cartridge containing all the reagents required to prepare a single library.
This platform utilizes AMPureXP DNA binding magnetic beads to purify fragmented DNA and perform size selection, replacing the need for column purification and gel-based size selection. There are two size range options available, 100-400bp or 300-600bp. There is also the choice to perform no size selection. When complete samples will be ready for PCR Enrichment and clean-up to complete the library prep process.
For more information on the SPRI-Works system please visit www.spriworks.com
Sample Submission
All samples submitted for direct SPRI-Works library preparation must be submitted with user supplied adapter mix. Please note that samples will need to be submitted separately for Illumina sequencing.
