BioMicroCenter:SPRI-Works: Difference between revisions
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==Sample Submission== | ==Sample Submission== | ||
All samples submitted for direct SPRI-Works library preparation | All samples submitted for direct SPRI-Works library preparation must be submitted with '''user supplied''' adapter mix. Please note that samples will need to be submitted separately for Illumina sequencing. | ||
[[BioMicroCenter:Forms#Downloads|Sample Submission Forms]] | [[BioMicroCenter:Forms#Downloads|Sample Submission Forms]] |
Revision as of 12:51, 2 August 2010
HOME -- | SEQUENCING -- | LIBRARY PREP -- | HIGH-THROUGHPUT -- | COMPUTING -- | OTHER TECHNOLOGY |
The BMC is currently testing the Beckman SPRIworks library preparation system. This platform is a fully automated library preparation system for Illumina sequencing and supports all Illumina applications except for smallRNA-Seq. It performs all of the traditional Illumina protocol steps listed on the Illumina Library Preparation page, except for Enrichment, can prepare up to ten libraries for Illumina sequencing in 5 hours.
How it Works
The SPRI-works system consists of three main components: the SPRI-TE Nucleic Acid Extractor, a method card that controls liquid handling, and a cartridge containing all the reagents required to prepare a single library.
This platform utilizes DNA binding magnetic beads to purify fragmented DNA and perform size selection, replacing the need for column purification and gel-base size selection. There are two size range options available, 100-400bp or 300-600bp. There is also the choice to perform no size selection
Sample Submission
All samples submitted for direct SPRI-Works library preparation must be submitted with user supplied adapter mix. Please note that samples will need to be submitted separately for Illumina sequencing.