BioMicroCenter:Sequencing Quality Control: Difference between revisions
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Below are some of the most common techniques used for QCing samples for sequencing: | Below are some of the most common techniques used for QCing samples for sequencing: | ||
* [[BioMicroCenter:2100BioAnalyzer|Bioanalyzer]] - | * [[BioMicroCenter:2100BioAnalyzer|Bioanalyzer]] - suggested for samples exceeding 10nM although has shown to be less reliable at lower concentrations. Available at the BMC for $10 per sample | ||
* | * RT-PCR, SYBERgreen assay - coming soon to the BMC!! | ||
* | * Nanodrop - not recommended for sequencing samples because this method has a detection limit of about 12nM to 22uM and has shown to be less reliable on the lower end of this range. | ||
* | * Qubit - not tested at the BMC but information about this application can be found at http://openwetware.org/index.php?title=BioMicroCenter:Sequencing_Quality_Control&action=edit&redlink=1 | ||
Revision as of 11:54, 8 January 2009
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Quality Control for Samples Submitted for Illumina Sequqncing
When submitting samples to the BMC for Illumina sequencing it is very important to supply us with a reliable measurement of the concentration and quantity of starting sample. Having this information allows us to insure reliability of cluster density to maximize the number of reads per lane and therefore maximize the quality of data produced. QC can either be run before sample submission and specified upon testing request or can be run by the BMC. We strongly recommend that you allow us to run QC of samples.
Below are some of the most common techniques used for QCing samples for sequencing:
- Bioanalyzer - suggested for samples exceeding 10nM although has shown to be less reliable at lower concentrations. Available at the BMC for $10 per sample
- RT-PCR, SYBERgreen assay - coming soon to the BMC!!
- Nanodrop - not recommended for sequencing samples because this method has a detection limit of about 12nM to 22uM and has shown to be less reliable on the lower end of this range.
- Qubit - not tested at the BMC but information about this application can be found at http://openwetware.org/index.php?title=BioMicroCenter:Sequencing_Quality_Control&action=edit&redlink=1
