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{{BioMicroCenter}}
{{BioMicroCenter}}


==TECAN FREEDOM EVO 150==
''' ** All users must be trained before being allowed to use the equipment **'''<BR><BR>
The BioMicro Center currently hosts three automated liquid handlers: two [https://lifesciences.tecan.com/products/liquid_handling_and_automation/freedom_evo_series Tecan Freedom EVO 150s] and one [https://www.ttplabtech.com/products/liquid-handling/mosquito-hv/ SPT Labtech Mosquito HV]. One EVO is optimized for cherry picking applications while the other is optimized for sample processing and assay set-up. The Tecans and Mosquito in the BioMicro Center are available to MIT researchers for use in their research. The Tecans may be used for BL1 work but are not set up for doing work on tissue culture or other samples requiring sterile environments. For users requiring higher throughput or BL2/BL2+  a new High-Throughput core has been developed by the [http://ki.mit.edu/sbc Koch Institute.] <BR><BR>


  ** All users must be trained before being allowed to use the Evo **
The Tecans are offered as a walk up service or as an assisted service. Due to the steep learning curves on the robots, we strongly encourage users to allow BMC staff to assist them in automating protocols and programming the instruments and then simply execute the programs as a walk up. Machine downtime is extremely limited and there is minimal time for practice runs.


[[Image:Tn_PR004749.jpg|right|97px]]
== JOHNNY 5 ==
The BioMicro Center currently hosts a Tecan Freedom EVO 150 The EVO is a highly-versatile device equipped with a four-channel liquid handling arm and a 96-channel pipette arm.  The instrument supports a wide variety of fluid transfer operations for all sizes of microplates as well as 150mm dishes and can perform operations including reagent addition, continuous reagent dispensing, sample transfer, plate replication, plate-to-plate transfer, serial dilutions, and within-well mixing.  The EVO can accurately handle volumes between 1 and 500 µl and has the ability to perform virtually any routine liquid transfer, replacing manual pipetting. The Freedom EVO is user-friendly and runs the EVOware software package. The EVOware package allows for the creation of a virtual image of the platform and allows the scientist to easily implement methods with a drag-and-drop graphical user interface.
{|
|- style="vertical-align: top;"
| style="width:350px;" |
  {| class="wikitable" border=1
  |-
  ! style="text-align: center; width: 150px;" | Instrument
  ! style="text-align: center; width: 300px;" | Tecan EVO 150
  |-
  | style="text-align: center; height: 2em;" | USAGE
  | style="text-align: center; height: 2em;" | Walkup - MIT only <br> Approval Required
  |-
  | style="text-align: center; height: 2em;" | UNIT
  | style="text-align: center; height: 2em;" | Per hour + Consumables
  |-
  | style="text-align: center; height: 2em;" | ARMS
  | style="text-align: left; height: 2em;" |
* LiHa span4 (2&micro;L-200&micro;L)
* MCA96 (50&micro;L/200&micro;L tips)
  |-
  | style="text-align: center; height: 2em;" | CARRIERS
  | style="text-align: left; height: 2em;" |
* 1 fixed tip washer
* 6 15ml tube carriers
* 3 temperature controlled SBS carriers
* 24 non-temperature controlled SBS carriers
* 4 racks for 1.5ml tubes (24 each)
* UV light for decontamination
  |-
  | style="text-align: center; height: 2em;" | SCHEDULE USAGE
  | style="text-align: center; height: 2em;" | [https://mit.ilabsolutions.com/equipment/show/262347/?tab=schedule ilabs]
  |-
  | style="text-align: center; height: 2em;" | NEW USERS
  | style="text-align: center; height: 2em;" | New users should request training by emailing the [mailto:biomicro@mit.edu  BioMicro Center team].
  |-
  | style="text-align: center; height: 2em;" | DONATED BY
  | style="text-align: center; height: 2em;" | Department of Biology
  |}
|
<br>
:"Johnny5" or "J5" is the BioMicro Center robot optimized for cherry picking. It is a highly-versatile device equipped with a four-channel liquid handling arm (LiHa) and a 96-channel pipette arm (MCA96).  The instrument supports a wide variety of fluid transfer operations for all sizes of microplates as well as 150mm dishes and can perform operations including reagent addition, continuous reagent dispensing, sample transfer, plate replication, plate-to-plate transfer, serial dilutions, and within-well mixing.  The EVO can accurately handle volumes between 2 and 200 µl and has the ability to perform virtually any routine liquid transfer, replacing manual pipetting. <BR><BR>


A second EVO150 will be arriving in the BioMicro Center in June 2010. This robot will have several additional features focused on screening 96 and 384 well plates. These include a 96-channel pipette arm, a remote manipulator arm, plate and tip stackers, plate shakers and incubators, etc. For more information about the Tecans, please email[[BioMicroCenter:People|Stuart Levine]]
:J5 is used by the BioMicro Center primarily for plate normalization as part of high-throughput library production. Users should plan experiments in advance to coordinate usage with BMC staff.<BR>
<br>
:::::::[[IMAGE:BMC_Johnny5.jpg | inline | 300px]] &nbsp;&nbsp;&nbsp;&nbsp; [[IMAGE:BMC_Johnny5_robot.jpg | inline | 110px]]
|
|}


[[Image:BioMicroCenter_Tecan2_Sim.png|frame|thumb|400px|alt=TecanImage|Simulated view of new Tecan EVO150]]
== B9 ==
{|
|- style="vertical-align: top;"
| style="width:350px;" |
  {| class="wikitable" border=1
  |-
  ! style="text-align: center; width: 150px;" | Instrument
  ! style="text-align: center; width: 325px;" | Tecan EVO 150
  |-
  | style="text-align: center; height: 2em;" | USAGE
  | style="text-align: center; height: 2em;" | Walkup - MIT only <br> Approval Required
  |-
  | style="text-align: center; height: 2em;" | UNIT
  | style="text-align: center; height: 2em;" | Per hour + Consumables
  |-
  | style="text-align: center; height: 2em;" | ARMS
  | style="text-align: left; height: 2em;" |
* MCA96 (50&micro;L/200&micro;L tips)
* RoMa (Robotic Manipulator)
  |-
  | style="text-align: center; height: 2em;" | CARRIERS
  | style="text-align: left; height: 2em;" |
* 1 rack for 2 stacks of nested tips with chute
* 3 temperature controlled SBS format positions on one carrier.
* 16 SBS format positions on 4 carrier
<!-- * [http://www.tecan.com/platform/apps/product/index.asp?MenuID=3416&ID=7010&Menu=1&Item=21.1.9.26 TeVac vacuum manifold] -->
* [http://www.tecan.com/platform/apps/product/index.asp?MenuID=1399&ID=973&Menu=1&Item=21.1.9.11 Vortexer (Te-Shake)]
* 4 on deck racks for short plates
* Shaking Incubator
* [http://www.tecan.com/platform/apps/product/index.asp?MenuID=1214&ID=611&Menu=1&Item=21.1.9.13 4 Stackers (TeStack)]
* Alpaqua 96-well Magnets
* 384-well Magnets
  |-
  | style="text-align: center; height: 2em;" | SCHEDULE USAGE
  | style="text-align: center; height: 2em;" | [https://mit.ilabsolutions.com/equipment/show/262347/?tab=schedule ilabs]
  |-
  | style="text-align: center; height: 2em;" | NEW USERS
  | style="text-align: center; height: 2em;" | New users should request training by emailing the [mailto:biomicro@mit.edu  BioMicro Center team].
  |-
  | style="text-align: center; height: 2em;" | DONATED BY
  | style="text-align: center; height: 2em;" | Dr. WENDY GILBERT (now at Yale)
  |}
|
<br>
:"B9" is the BioMicro Center EVO150 optimized for nucleic acid cleans and quantification assays. B9 has a 96-channel pipette arm (MCA96) and a robotic manipulator (RoMa) arm for moving plates around the deck. This instrument supports a broad range of fluid transfer operations including reagent addition, sample transfer, plate-to-plate transfer, serial dilutions, and within-well mixing. This robot contains a waste chute and 4 plate stackers which combined with the RoMa arm can be used to add or remove plates makes B9 a highly dynamic automation platform. B9 was donated to the BioMicro Center by Dr. Wendy Gilbert through a shared instrumentation grant from the NIH. <BR><BR>


== SOFTWARE ==
:B9 is regularly used by the BioMicro Center for nucleic acid cleans, SYBR and qPCR assay setup, and Illumina Library preparation. <br>
<br>
:::::::[[IMAGE:BMC_B9.png | inline | 300px]] &nbsp;&nbsp; [[Image:BMC_B9_robot.png | inline | 125px]]
|
|}
 
== SPT LABTECH MOSQUITO HV ==
{|
{|
|[[Image:BioMicroCenter-EVOware.png|200px]]
|- style="vertical-align: top;"
| The Tecan EVO currently runs using the [http://www.tecan.com/platform/apps/product/index.asp?MenuID=1458&ID=1084&Menu=1&Item=21.7.1 EVOware] software package. EVOware can be run in either of two modes: directly connected to the Tecan EVO to allow control of the robot, or on your desktop computer in a simulation mode. Simulation mode is extremely useful in that it allows the user to pre-design the steps in their program. All users must download EVOware and design their protocols to be brought to the EVO.
| style="width:350px;" |
  {| class="wikitable" border=1
  |-
  ! style="text-align: center; width: 150px;" | Instrument
  ! style="text-align: center; width: 325px;" | Mosquito HV
  |-
  | style="text-align: center; height: 2em;" | USAGE
  | style="text-align: center; height: 2em;" | Not available as a walkup service
  |-
  | style="text-align: center; height: 2em;" | UNIT
  | style="text-align: center; height: 2em;" | Included in high-throughput library preparation
  |-
  | style="text-align: center; height: 2em;" | ARMS
  | style="text-align: center; height: 2em;" | 8-tip disposable (0.2&micro;L - 5&micro;L)
  |-
  | style="text-align: center; height: 2em;" | CARRIERS
  | style="text-align: left; height: 2em;" |
* 5 SBS positions
* Humidifying chamber
  |-
  | style="text-align: center; height: 2em;" | SCHEDULE USAGE
  | style="text-align: center; height: 2em;" | Contact [[BioMicroCenter:People|Tatum Murdock]] for special requests
  |-
  | style="text-align: center; height: 2em;" | DONATED BY
  | style="text-align: center; height: 2em;" | SPT Labtech (on loan)
  |}
|
<br>
<br>
* [[BioMicroCenter:EVOware | Obtain a copy of EVOware]]
:The SPT Labtech Mosquito HV is a critical instrument in the BioMicro Center's goal of automated high-throughput library production. The accurate low volume pipetting offered by this liquid handler has allowed for miniaturization of a number of protocols. While the instrument does not provide a 'walk away' level of automation, programming the Mosquito is significantly easier than the Tecans and prototyping methods is much more rapid. <BR><BR>
:The Mosquito is used in all HTL protocols in the BioMicro Center.
|
<br>
[[Image:BMC_MOSQUITO.png | 300px]]
|}
|}


** Currently all programs must be approved by [[BioMicroCenter:People|Stuart Levine]] or Wendy Gilbert before being loaded onto the TECAN **
== verrity Chemagic 360 ==
 
{|
== LABWARE ==
|- style="vertical-align: top;"
Labware refers to the plastic consumables used by the robot. These include the tips, tubes, plates, etc. Each piece of labware must be individually prepared for the EVO and each program must refer to specific pieces of labware. Failure to properly set up labware can cause expensive damage to the machine and significant down time. The BioMicro Center will stock a standard set of labware that will be included in the "Base.xsd" file.
| style="width:350px;" |
 
  {| class="wikitable" border=1
[[BioMicroCenter:EVOlabware | LIST OF LABWARE]] - We are currently evaluating different types of labware. If you are interested in having a particular piece of labware, please make sure to edit the list.
  |-
 
  ! style="text-align: center; width: 150px;" | Instrument
== HARDWARE ==
  ! style="text-align: center; width: 325px;" | Chemagic360
* 4 channel liquid handling arm (LiHa)
  |-
* 96 channel multichannel arm (MCA)
  | style="text-align: center; height: 2em;" | USAGE
* Vacuum Manifold (TVac)
  | style="text-align: center; height: 2em;" | Available as a walkup service
* 2 thermal control plate racks (4C - ??C)
  |-
* 2 ambient plate racks
  | style="text-align: center; height: 2em;" | UNIT
 
  | style="text-align: center; height: 2em;" | Included in high-throughput sample preparation
== LOGISTICS ==
  |-
 
  | style="text-align: center; height: 2em;" | chemagic dispenser
 
  | style="text-align: center; height: 2em;" | 12-tip 3-row array
 
  |-
=== SCHEDULING ===
  | style="text-align: center; height: 2em;" | Rod Head
Access to the Tecan is available in 2 hour blocks. You can schedule use of the Tecan [[BioMicroCenter:Tecan_CAL|HERE]]
  | style="text-align: left; height: 2em;" | 96 well EM head
 
  |-
=== [[BioMicroCenter:Pricing|COST]] ===
  | style="text-align: center; height: 2em;" | SCHEDULE USAGE BY PURIFICATION TYPE
In order to defray the costs of the annual service contract, each session on the EVO will be charged '''$75.'''. Tips and 96 well plates will be available for purchase from the BioMicro center or obtained from your own lab. Additionally, training is required for all new users. A training session will be $100
  | style="text-align: center; height: 2em;" | Contact [[BioMicroCenter:People|Avanyish Toniappa]] a month ahead
  |-
  | style="text-align: center; height: 2em;" | DONATED BY
  | style="text-align: center; height: 2em;" |
* MIT Stem Cell Initiative
* Dr Jacqueline A. Lees
  |}
|
<br>
:The verrity (formerly Perkin-Elmer) Chemagic360 is a critical instrument in the BioMicro Center's goal of automated high-throughput library production. 96 tissue or cell lysates take up to 2 hours for a thorough bead based clean. This instrument provides 'walk away' automation, but does require setup steps such as creating lysates before loading, and unloading the instrument properly. There are no optional programming options. <BR><BR>
:If a kit for a specific purification needs to be ordered, it will take a month to arrive.
|
<br>
[[Image:Chemagic360.jpg | 300px]]
|}

Latest revision as of 12:12, 18 April 2024

HOME -- SEQUENCING -- LIBRARY PREP -- HIGH-THROUGHPUT -- COMPUTING -- OTHER TECHNOLOGY

** All users must be trained before being allowed to use the equipment **

The BioMicro Center currently hosts three automated liquid handlers: two Tecan Freedom EVO 150s and one SPT Labtech Mosquito HV. One EVO is optimized for cherry picking applications while the other is optimized for sample processing and assay set-up. The Tecans and Mosquito in the BioMicro Center are available to MIT researchers for use in their research. The Tecans may be used for BL1 work but are not set up for doing work on tissue culture or other samples requiring sterile environments. For users requiring higher throughput or BL2/BL2+ a new High-Throughput core has been developed by the Koch Institute.

The Tecans are offered as a walk up service or as an assisted service. Due to the steep learning curves on the robots, we strongly encourage users to allow BMC staff to assist them in automating protocols and programming the instruments and then simply execute the programs as a walk up. Machine downtime is extremely limited and there is minimal time for practice runs.

JOHNNY 5

Instrument Tecan EVO 150
USAGE Walkup - MIT only
Approval Required
UNIT Per hour + Consumables
ARMS
  • LiHa span4 (2µL-200µL)
  • MCA96 (50µL/200µL tips)
CARRIERS
  • 1 fixed tip washer
  • 6 15ml tube carriers
  • 3 temperature controlled SBS carriers
  • 24 non-temperature controlled SBS carriers
  • 4 racks for 1.5ml tubes (24 each)
  • UV light for decontamination
SCHEDULE USAGE ilabs
NEW USERS New users should request training by emailing the BioMicro Center team.
DONATED BY Department of Biology


"Johnny5" or "J5" is the BioMicro Center robot optimized for cherry picking. It is a highly-versatile device equipped with a four-channel liquid handling arm (LiHa) and a 96-channel pipette arm (MCA96). The instrument supports a wide variety of fluid transfer operations for all sizes of microplates as well as 150mm dishes and can perform operations including reagent addition, continuous reagent dispensing, sample transfer, plate replication, plate-to-plate transfer, serial dilutions, and within-well mixing. The EVO can accurately handle volumes between 2 and 200 µl and has the ability to perform virtually any routine liquid transfer, replacing manual pipetting.

J5 is used by the BioMicro Center primarily for plate normalization as part of high-throughput library production. Users should plan experiments in advance to coordinate usage with BMC staff.


inline      inline

B9

Instrument Tecan EVO 150
USAGE Walkup - MIT only
Approval Required
UNIT Per hour + Consumables
ARMS
  • MCA96 (50µL/200µL tips)
  • RoMa (Robotic Manipulator)
CARRIERS
  • 1 rack for 2 stacks of nested tips with chute
  • 3 temperature controlled SBS format positions on one carrier.
  • 16 SBS format positions on 4 carrier
  • Vortexer (Te-Shake)
  • 4 on deck racks for short plates
  • Shaking Incubator
  • 4 Stackers (TeStack)
  • Alpaqua 96-well Magnets
  • 384-well Magnets
SCHEDULE USAGE ilabs
NEW USERS New users should request training by emailing the BioMicro Center team.
DONATED BY Dr. WENDY GILBERT (now at Yale)


"B9" is the BioMicro Center EVO150 optimized for nucleic acid cleans and quantification assays. B9 has a 96-channel pipette arm (MCA96) and a robotic manipulator (RoMa) arm for moving plates around the deck. This instrument supports a broad range of fluid transfer operations including reagent addition, sample transfer, plate-to-plate transfer, serial dilutions, and within-well mixing. This robot contains a waste chute and 4 plate stackers which combined with the RoMa arm can be used to add or remove plates makes B9 a highly dynamic automation platform. B9 was donated to the BioMicro Center by Dr. Wendy Gilbert through a shared instrumentation grant from the NIH.

B9 is regularly used by the BioMicro Center for nucleic acid cleans, SYBR and qPCR assay setup, and Illumina Library preparation.


inline    inline

SPT LABTECH MOSQUITO HV

Instrument Mosquito HV
USAGE Not available as a walkup service
UNIT Included in high-throughput library preparation
ARMS 8-tip disposable (0.2µL - 5µL)
CARRIERS
  • 5 SBS positions
  • Humidifying chamber
SCHEDULE USAGE Contact Tatum Murdock for special requests
DONATED BY SPT Labtech (on loan)


The SPT Labtech Mosquito HV is a critical instrument in the BioMicro Center's goal of automated high-throughput library production. The accurate low volume pipetting offered by this liquid handler has allowed for miniaturization of a number of protocols. While the instrument does not provide a 'walk away' level of automation, programming the Mosquito is significantly easier than the Tecans and prototyping methods is much more rapid.

The Mosquito is used in all HTL protocols in the BioMicro Center.


verrity Chemagic 360

Instrument Chemagic360
USAGE Available as a walkup service
UNIT Included in high-throughput sample preparation
chemagic dispenser 12-tip 3-row array
Rod Head 96 well EM head
SCHEDULE USAGE BY PURIFICATION TYPE Contact Avanyish Toniappa a month ahead
DONATED BY
  • MIT Stem Cell Initiative
  • Dr Jacqueline A. Lees


The verrity (formerly Perkin-Elmer) Chemagic360 is a critical instrument in the BioMicro Center's goal of automated high-throughput library production. 96 tissue or cell lysates take up to 2 hours for a thorough bead based clean. This instrument provides 'walk away' automation, but does require setup steps such as creating lysates before loading, and unloading the instrument properly. There are no optional programming options.

If a kit for a specific purification needs to be ordered, it will take a month to arrive.


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