BioMicroCenter:Tecan Freedom Evo

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TECAN FREEDOM EVO 150

 ** All users must be trained before being allowed to use the Evo **

The BioMicro Center currently hosts a Tecan Freedom EVO 150 The EVO is a highly-versatile device equipped with a four-channel liquid handling arm and a 96-channel pipette arm. The instrument supports a wide variety of fluid transfer operations for all sizes of microplates as well as 150mm dishes and can perform operations including reagent addition, continuous reagent dispensing, sample transfer, plate replication, plate-to-plate transfer, serial dilutions, and within-well mixing. The EVO can accurately handle volumes between 1 and 500 µl and has the ability to perform virtually any routine liquid transfer, replacing manual pipetting. The Freedom EVO is user-friendly and runs the EVOware software package. The EVOware package allows for the creation of a virtual image of the platform and allows the scientist to easily implement methods with a drag-and-drop graphical user interface.

A second EVO150 will be arriving in the BioMicro Center in June 2010. This robot will have several additional features focused on screening 96 and 384 well plates. These include a 96-channel pipette arm, a remote manipulator arm, plate and tip stackers, plate shakers and incubators, etc. For more information about the Tecans, please emailStuart Levine

Simulated view of new Tecan EVO150

SOFTWARE

The Tecan EVO currently runs using the EVOware software package. EVOware can be run in either of two modes: directly connected to the Tecan EVO to allow control of the robot, or on your desktop computer in a simulation mode. Simulation mode is extremely useful in that it allows the user to pre-design the steps in their program. All users must download EVOware and design their protocols to be brought to the EVO. Obtain a copy of EVOware

** Currently all programs must be approved by Stuart Levine or Wendy Gilbert before being loaded onto the TECAN **

LABWARE

Labware refers to the plastic consumables used by the robot. These include the tips, tubes, plates, etc. Each piece of labware must be individually prepared for the EVO and each program must refer to specific pieces of labware. Failure to properly set up labware can cause expensive damage to the machine and significant down time. The BioMicro Center will stock a standard set of labware that will be included in the "Base.xsd" file.

LIST OF LABWARE - We are currently evaluating different types of labware. If you are interested in having a particular piece of labware, please make sure to edit the list.

HARDWARE

• 4 channel liquid handling arm (LiHa)
• 96 channel multichannel arm (MCA)
• Vacuum Manifold (TVac)
• 2 thermal control plate racks (4C - ??C)
• 2 ambient plate racks

LOGISTICS

SCHEDULING

Access to the Tecan is available in 2 hour blocks. You can schedule use of the Tecan HERE

COST

In order to defray the costs of the annual service contract, each session on the EVO will be charged $75.. Tips and 96 well plates will be available for purchase from the BioMicro center or obtained from your own lab. Additionally, training is required for all new users. A training session will be $100