BioMicroCenter:Tecan Freedom Evo
TECAN FREEDOM EVO 150
The TECAN Freedom EVO 150 is a highly versatile device capable of automating dozens of different applications. Our Tecan is currently equipped with a four-channel liquid handling arm and a 96 tip multi-channel arm. The instrument supports a wide variety of fluid transfer operations for both 96 and 384-well microplates including reagent addition, continuous reagent dispensing, sample transfer, plate replication, plate-to-plate transfer (96 to 96, 384 to 384, 96 to 384) serial dilutions, and within-well mixing. The Tecan Freedom Evo 150 can accurately handle volumes between 1 and 200 ul.
LOCATION and TRAINING
The TECAN Freedom EVO 150 is currently located in 68-4XX. The TECAN will be relocated to the BioMicro Center in early 2009. Because of the flexibility of the EVO system, all users are required to be trained prior to using the robot. Training can be scheduled in the BioMicro Center with Stuart Levine.
The BioMicro Center is currently evaluating all of the different plastics to be used in the EVO. This is very important because a significant set up is required for handling each different type of tube, tip or plate, each of which must be configured separately.. Failure to use "approved" plastics can cause expensive damage to the machine and significant down time disrupting many experiments. BMC approved plastics will be available in the BioMicro Center at cost.
Please enter the types of plastics you would like to use along with their catalog number and supplier.
VWR brand 1-200 ul tips VWR 30128-378
VWR brand 1-10 ul tips VWR 53503-810
Pierce/Thermo 96 well plate black, U-bottom VWR 62403-152
Axygen 1.7ml microcentrifuge tube VWR 10011-722
The Tecan EVO is controlled by EVOWARE STANDARD. The software itself is free and can be downloaded onto any PC. When not connected to the robot, EVOWARE runs in a simulation mode. This mode can be used to design all of your routines for the robot which can later be exported to the main robot. An image of the EVOWARE software will be placed online soon for download. In the mean time, the CDs are available by contacting Stephen Goldman.
Access to the Tecan is available in 2 hour blocks. You can schedule use of the Tecan HERE
NOTE: Until March 2009, access to the Tecan will be restricted. Please make sure to contact Stuart Levine after you have scheduled time to be sure the room will be open
In order to defray the costs of the annual service contract, each session on the EVO will be charged $75.. Tips and 96 well plates can be purchased from the BioMicro center or obtained from your own lab. Additionally, training is required for all new users. A training session will be $100
** All users must be trained before being allowed to use the Evo **