BioMicroCenter:Tecan Freedom Evo

From OpenWetWare

Revision as of 15:26, 20 May 2012 by Stuart S. Levine (Talk | contribs)
Jump to: navigation, search


 ** All users must be trained before being allowed to use the Tecans **

The BioMicro Center currently hosts two Tecan Freedom EVO 150s named Johnny5 and B9. Johnny5 is optimized for cherry picking applications while B9 is optimized for screening.



"Johnny5" is a highly-versatile device equipped with a four-channel liquid handling arm (LiHa) and a 96-channel pipette arm (MCA96). The instrument supports a wide variety of fluid transfer operations for all sizes of microplates as well as 150mm dishes and can perform operations including reagent addition, continuous reagent dispensing, sample transfer, plate replication, plate-to-plate transfer, serial dilutions, and within-well mixing. The EVO can accurately handle volumes between 1 and 500 µl and has the ability to perform virtually any routine liquid transfer, replacing manual pipetting.


Johnny 5 has the following arms:

  • LiHa (Liquid handler): 4 fixed conductivity sensing tips. 1-500 µl
  • MCA (Multichannel Arm): 96 Tip head uses disposable tips (50 or 200 µl)


Johnny 5's deck is configured with the following carriers.

  • 1 fixed tip washer.
  • 3 15ml tube carriers.
  • 2 temperature controlled carriers (holds 6 plates)
  • 3 non-temperature controlled carriers (hold 10 plates)
  • 3 spots for tops.

SCHEDULE TIME ON JOHNNY 5 Note, time must be booked in 2 hour increments.




The second EVO150 arrived in the BioMicro Center in June 2010. This robot has several additional features focused on screening 96 and 384 well plates. These include a 96-channel pipette arm, a remote manipulator arm, plate and tip stackers, plate shakers and incubators, etc. For more information about the Tecans, please email Stuart Levine

Simulated view of new Tecan EVO150
Simulated view of new Tecan EVO150


The Tecan EVO currently runs using the EVOware software package. EVOware can be run in either of two modes: directly connected to the Tecan EVO to allow control of the robot, or on your desktop computer in a simulation mode. Simulation mode is extremely useful in that it allows the user to pre-design the steps in their program. All users must download EVOware and design their protocols to be brought to the EVO.

 All programs must be approved by Stuart Levine or Wendy Gilbert before being loaded onto the TECAN. 


Labware refers to the plastic consumables used by the robot. These include the tips, tubes, plates, etc. Each piece of labware must be individually prepared for the EVO and each program must refer to specific pieces of labware. Failure to properly set up labware can cause expensive damage to the machine and significant down time. The BioMicro Center will stock a standard set of labware that will be included in the "Base.xsd" file.

LIST OF LABWARE - We are currently evaluating different types of labware. If you are interested in having a particular piece of labware, please make sure to edit the list.


  • 4 channel liquid handling arm (LiHa)
  • 96 channel multichannel arm (MCA)
  • Vacuum Manifold (TVac)
  • 2 thermal control plate racks (4C - ??C)
  • 2 ambient plate racks



Access to the Tecan liquid handling robots is available in 2 hour blocks. You can schedule use of either robot by clicking on the link below.


In order to defray the costs of the annual service contract, each 2 hour session on the EVO will be charged $50.. Tips and 96 well plates are available for purchase from the BioMicro center or obtained from your own lab. Additionally, training is required for all new users. A training session is $100

Personal tools