BioMicroCenter:Varioskan: Difference between revisions

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{{BioMicroCenter}}
{{BioMicroCenter}}


[[Image:BioMicroCenter_Varioskan.jpg|right]]
== Varioskan Flash Reader ==
The Thermo Scientific Varioskan Flash Spectral Scanning Multimode Reader is a highly flexible plate reader capable of handling fluorometric (excitation/emission), luminometric  (emission) and photometric (absorbance) measurements in 96+ well format. The Varioskan can handle a broad range of applications from reporter assays to FRET. The Varioskan is equipped with three on board fluid dispensers, incubator and shaker to allow for real time assays. Coupled with the [[BioMicroCenter:Tecan_Freedom_Evo|Tecan EVO]], the Varioskan should allow major improvements in high throughput experiment design. Currently the Varioskan is located in '''68-252d'''. Please speak with '''Kevin Thai''' at the BioMicro Center about training if you wish to use the machine.
<br>
* On August 4th, 2010, the Varioskan will be relocated to 68-316. At that time a $10/plate charge will be implemented to cover the charge of the service contract. Plates requiring minimal time on the machine (<10 minutes) can be scanned without charge but may not be scheduled on the calender and must give priority to scheduled users. We will post an announcement before we plan to move the instrument so all users are aware of the move ahead of time.


<br>
== PLATE READER ==
'''[[BioMicroCenter:Vario_CAL|Schedule time on the Varioskan]]'''
{| align=left
<br><br>
|- style="vertical-align: top;"
|style="width: 300px;"|
{| class="wikitable" border=1
  ! Instrument || ThermoFisher Varioskan
  |-
  | TYPE || WALKUP - MIT only
  |-
  | UNIT || Per hour in 10' increments
  |-
  | FORMAT || 96w/394w
  |-
  | FLUOROMETRY
  |
* 6-1536w
* Excite: 200-1000nm
* Emission: 270-840nm
* Sensitivity: <0.4fmol fluorescein/w
* Dynamic Range: >6 decades
  |-
  | LUMINOMETRY
  |
* 6-384w spectral scan
* 360-670nm
* 270-840nm spectral scan
* Sensitivity: < amol ATP/w
* Dynamic Range: >7 decades
  |-
  | PHOTOMETRY <BR> ABSORBANCE
  |
* 6-384w
* Wavelength: 200-1000nm
* Sensitivity: 0.003Abs +/-2%
* Dynamic Range: 0-4Abs @450nm
  |-
  | SIGNUP || [https://mit.ilabsolutions.com/equipment/show/262715/?tab=schedule ilabs]
  |-
  | NEW USERS ||  New users should request training by emailing biomicro@mit.edu
  |-
  | DONATED BY || Prof. Jeroen Saeij.
|}
|
The Thermo Scientific Varioskan Flash Spectral Scanning Multimode Reader is a highly flexible plate reader capable of handling fluorometric (excitation/emission), luminometric  (emission) and photometric (absorbance) measurements in 96+ well format. The Varioskan can handle a broad range of applications from reporter assays to FRET. The Varioskan is equipped with three on board fluid dispensers, incubator and shaker to allow for real time assays. Coupled with the [[BioMicroCenter:Tecan_Freedom_Evo|Tecan EVO]], the Varioskan should allow major improvements in high throughput experiment design. Currently the Varioskan is located in '''68-322'''. <BR><BR>


== Specifications ==
''The Varioskan supports:''
 
{|border="1"
|+
!  !! Fluorometry !! Luminometry !! Photometry
|-
| '''Plate Density:'''
| 6-1536 well
| 6-1536 well <br> 6-386 well spectral scan
| 6-384 well
|-
| '''Wavelength:'''
| Ex: 200-1000 nm<br>Em: 270-840 nm
| 360-670 nm <br> 270-840 nm spectral scan
| 200-1000 nM
|-
| ''' Sensitivity:'''
| <0.4 fmol flourescein/well
| < amol ATP/well
| +/- 2% or 0.003 Abs
|-
| '''Dynamic range:'''
| > 6 decades
| > 7 decades
| 0-4 Abs @ 450 nm +/-2%
|}
 
===Features:===
* 3 onboard liquid dispensers
* 3 onboard liquid dispensers
* 1ul – 10ml dispensing volumes
* 1ul – 10ml dispensing volumes
* Temperature control from ambient + 4C – 45C
* Temperature control from ambient + 20C – 45C
* On board orbital shaker
* On board orbital shaker


== Suggested Applications ==
The varioskan is used routinely in the BioMicro Center to measure the concentration of Illumina libraries using SYBRgreen. The Varioskan will also be used to measure concentrations of oligos from Dr.Oligo.<br><br>
''Suggested Assays:''
* Apoptosis assays
* Apoptosis assays
* Ca2+ flux assays
* Ca2+ flux assays
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* Cellular assays
* Cellular assays
* Cytotoxicity and ADMETox
* Cytotoxicity and ADMETox
* [[BioMicroCenter:Varioskan_DNA_Test|DNA concentration]]
* DNA concentration
* ELISA/FIA/TRF-ELISA assays
* ELISA/FIA/TRF-ELISA assays
* Enzyme kinetic studies
* Enzyme kinetic studies
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* Signal transduction
* Signal transduction
* Tryptophan and tyrosine UV fluorescence
* Tryptophan and tyrosine UV fluorescence
|
[[Image:BioMicroCenter_Varioskan.jpg|right]]<BR>
|}

Latest revision as of 09:38, 6 September 2023

HOME -- SEQUENCING -- LIBRARY PREP -- HIGH-THROUGHPUT -- COMPUTING -- OTHER TECHNOLOGY


PLATE READER

Instrument ThermoFisher Varioskan
TYPE WALKUP - MIT only
UNIT Per hour in 10' increments
FORMAT 96w/394w
FLUOROMETRY
  • 6-1536w
  • Excite: 200-1000nm
  • Emission: 270-840nm
  • Sensitivity: <0.4fmol fluorescein/w
  • Dynamic Range: >6 decades
LUMINOMETRY
  • 6-384w spectral scan
  • 360-670nm
  • 270-840nm spectral scan
  • Sensitivity: < amol ATP/w
  • Dynamic Range: >7 decades
PHOTOMETRY
ABSORBANCE
  • 6-384w
  • Wavelength: 200-1000nm
  • Sensitivity: 0.003Abs +/-2%
  • Dynamic Range: 0-4Abs @450nm
SIGNUP ilabs
NEW USERS New users should request training by emailing biomicro@mit.edu
DONATED BY Prof. Jeroen Saeij.

The Thermo Scientific Varioskan Flash Spectral Scanning Multimode Reader is a highly flexible plate reader capable of handling fluorometric (excitation/emission), luminometric (emission) and photometric (absorbance) measurements in 96+ well format. The Varioskan can handle a broad range of applications from reporter assays to FRET. The Varioskan is equipped with three on board fluid dispensers, incubator and shaker to allow for real time assays. Coupled with the Tecan EVO, the Varioskan should allow major improvements in high throughput experiment design. Currently the Varioskan is located in 68-322.

The Varioskan supports:

  • 3 onboard liquid dispensers
  • 1ul – 10ml dispensing volumes
  • Temperature control from ambient + 20C – 45C
  • On board orbital shaker

The varioskan is used routinely in the BioMicro Center to measure the concentration of Illumina libraries using SYBRgreen. The Varioskan will also be used to measure concentrations of oligos from Dr.Oligo.

Suggested Assays:

  • Apoptosis assays
  • Ca2+ flux assays
  • Cell proliferation
  • Cellular assays
  • Cytotoxicity and ADMETox
  • DNA concentration
  • ELISA/FIA/TRF-ELISA assays
  • Enzyme kinetic studies
  • Europium assays
  • FRET assays
  • TR-FRET assays
  • BRET assays
  • GPCR assays
  • Ion channel assays
  • Kinase assays
  • Multilabel assays
  • Reporter gene assays
  • Signal transduction
  • Tryptophan and tyrosine UV fluorescence

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