BioMicroCenter:Wafergen
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| - | [[Image:BioMicroCenter-Smartchip.jpg| | + | [[Image:BioMicroCenter-Smartchip.jpg|thumb|right|200px|Image of Wafergen's SmartChip]] |
The Wafergen SmartChip System is a highly flexible nanoliter qPCR instrument. The system is based on aluminum chips that contain a grod of 72x72 nanowells. Each well can hold up to 150ul of volume. The system itself is composed of two halves: a robot for loading the chips and a PCR machine for cycling and scanning the chips. | The Wafergen SmartChip System is a highly flexible nanoliter qPCR instrument. The system is based on aluminum chips that contain a grod of 72x72 nanowells. Each well can hold up to 150ul of volume. The system itself is composed of two halves: a robot for loading the chips and a PCR machine for cycling and scanning the chips. | ||
== REACTION SETUP == | == REACTION SETUP == | ||
| - | + | Wafergen reactions come in two flavors: prefilled arrays and "MyDesign". Prefilled arrays have specific primer sets laid out across the nanowells. MyDesign arrays are filled in two steps: cDNA followed by primers. Primers and cDNA are filled from specific 384 well plates in master mix already diluted to 1x. | |
== RUNNING THE CHIPS == | == RUNNING THE CHIPS == | ||
Revision as of 19:02, 11 February 2013
The Wafergen SmartChip System is a highly flexible nanoliter qPCR instrument. The system is based on aluminum chips that contain a grod of 72x72 nanowells. Each well can hold up to 150ul of volume. The system itself is composed of two halves: a robot for loading the chips and a PCR machine for cycling and scanning the chips.
Contents |
REACTION SETUP
Wafergen reactions come in two flavors: prefilled arrays and "MyDesign". Prefilled arrays have specific primer sets laid out across the nanowells. MyDesign arrays are filled in two steps: cDNA followed by primers. Primers and cDNA are filled from specific 384 well plates in master mix already diluted to 1x.
RUNNING THE CHIPS
DATA ANALYSIS
