Biomod/2011/Caltech/DeoxyriboNucleicAwesome/Gel Experiments

Thursday, April 25, 2024 Home Members Project Protocols Progress Discussion References

Gel Experiments Example Experiment 0-1 The number to the left of the dash in the title represents which block of tests it belongs to, and the number to the right of the dash represents it's identity within the block Gel Identity: The number to the left of the dash in this entry represents which gel the experiment will be observed on, and the right of the dash represents which lane it will occupy. Purpose: This explains why this particular experiment is necessary or what it shows. Equation: This shows the reagents and expected outcomes. Controls: These are the controls necessary to read the results. Positive Result: This is what we should observe to verify that this particular reaction worked as intended. Negative Result: This is what we expect not to see. If this occurs, we will need to explore further for the cause of failure. List of Experiments X + Y indicates strand X and strand Y exist independently in the same solution. (X - Y) indicates that strand X and strand Y bind to form a complex Block 1: Walker Binding to track Experiment 1-1 Gel Identity: Purpose: Equation: W + TR1 → (W - TR1) Controls: Positive Result: Negative Result: Block 2: Walker Triggering Mechanism Block 3: Random Walking Mechanism Block 4: Cargo Pickup Mechanism Block 5: Goal Triggering Mechanism Block 6: Cargo Drop Off Mechanism Block 7: Time-based Tests Block 8: Walker Goal Functionality Gel Layout