Biomod/2011/Caltech/DeoxyriboNucleicAwesome/Gel Experiments: Difference between revisions
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'''Equation:''' W + (TR<sub>2</sub> - PTR<sub>2</sub>) → (TR<sub>2</sub> - PTR<sub>2</sub>)<br> | '''Equation:''' W + (TR<sub>2</sub> - PTR<sub>2</sub>) → (TR<sub>2</sub> - PTR<sub>2</sub>)<br> | ||
'''Purpose:''' To show that Track 2's probe does not interfere with the binding of the Walker to track 2.<br> | '''Purpose:''' To show that Track 2's probe does not interfere with the binding of the Walker to track 2.<br> | ||
'''Controls:''' W, TR<sub>2</sub>, PTR<sub>2</sub>, (TR<sub>2</sub> - PTR<sub>2</sub>)<br> | '''Controls:''' W, TR<sub>2</sub>, PTR<sub>2</sub>, (TR<sub>2</sub> - PTR<sub>2</sub>)<br> | ||
'''Positive Result:''' We should see a dark band different from the controls. We may see light bands corresponding to the reagents.<br> | '''Positive Result:''' We should see a dark band different from the controls. We may see light bands corresponding to the reagents.<br> | ||
'''Negative Result:''' We should not see dark bands corresponding to any of the control lanes. This indicates that the strands did not bind as intended. The controls should give us enough information to determine which (if any) of the bindings is preferred by the system.<br> | '''Negative Result:''' We should not see dark bands corresponding to any of the control lanes. This indicates that the strands did not bind as intended. The controls should give us enough information to determine which (if any) of the bindings is preferred by the system.<br> | ||
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====Experiment 3-3==== | |||
'''Gel Identity:''' 2-8<br> | |||
'''Equation:''' (W - TR<sub>1</sub>) + (TR<sub>2</sub> - PTR<sub>2</sub>) ←→ (W - TR<sub>2</sub> - PTR<sub>2</sub>) + TR<sub>1</sub><br> | |||
'''Purpose:''' To show that the Walker can move between tracks, specifically from Track 1 to Track 2. The system should equalize with walkers on both tracks.<br> | |||
'''Controls:''' W, TR<sub>1</sub>, TR<sub>2</sub>, PTR<sub>2</sub>, (W - TR<sub>1</sub>), (TR<sub>2</sub> - PTR<sub>2</sub>), (W - TR<sub>2</sub> - PTR<sub>2</sub>)<br> | |||
'''Positive Result:''' We should see a dark bands corresponding to all of the reagents and products.<br> | |||
'''Negative Result:''' We should not see any other dark bands. These would indicate that This indicates that either the Walker did not move as intended or that the movement interfered with another binding. The controls should give us enough information to determine which (if any) of the bindings is preferred by the system.<br> | |||
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Revision as of 15:20, 1 July 2011
Thursday, March 28, 2024
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Gel ExperimentsExampleExperiment 0-1The number to the left of the dash in the title represents which block of tests it belongs to, and the number to the right of the dash represents it's identity within the block List of ExperimentsX + Y indicates strand X and strand Y exist independently in the same solution. Block 1: Walker Binding to trackExperiment 1-1Gel Identity: 1-6 Experiment 1-2Gel Identity: 1-7 Experiment 1-3Gel Identity: 1-12 Block 2: Walker Triggering MechanismExperiment 2-1Gel Identity: 1-8 Experiment 2-2Gel Identity: 1-9 Experiment 2-3Gel Identity: 1-10 Experiment 2-4Gel Identity: 1-11 Block 3: Random Walking MechanismExperiment 3-1Gel Identity: 2-5 Experiment 3-2Gel Identity: 2-6 Experiment 3-3Gel Identity: 2-8 Block 4: Cargo Pickup MechanismBlock 5: Goal Triggering MechanismBlock 6: Cargo Drop Off MechanismBlock 7: Time-based TestsBlock 8: Walker Goal FunctionalityGel Layout |