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| == SPEX==
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| 1. Turn on the lamp and wait for 30 min for the lamp to warm up.
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| 2. Launch the software: Instrument control center
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| 3. Temperature set up
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| Click the third square icon “Visual setup”
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| Temperature control icon: Turn on and Set to 25 degreeC
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| Choose T bath (internal).
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| 4. Wash the cuvettes.
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| Distilled Water (DW) (10 times) 70% ethanol (twice) DW (5 times) 70% ethanol (once)
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| Air dry the cuvettes for 1 h.
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| 5. Clean the cuvettes using lens paper.
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| 6. Click second square icon “real time display”.
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| High voltage: HV on, S 950, T 950
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| Bandpass : 2
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| 7. Click the last icon “run CWA”.
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| Open the file “ROX_CWA”
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| Optional mode: kinetics, Integration time: 10s
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| Define: Repeat acquisition: 1 min (minimum) for a total of ~ min, depending on how long you would like to run the experiment.
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| 8. Mixing: Mix the sample with 1X TE/Mg++ buffer inside cuvettes by pipetting for 30 times.
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| 9. Start acquisition. Run sample.
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| The data should not exceed 1 million (1000,000).
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| 10. To add more samples (for example, walker triggers) in the middle of SPEX experiment
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| When the status is “waiting”, click “stop”.
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| Add samples and mix. Replace cuvettes into the SPEX machine.
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| 11. Save the data
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| When the status is “waiting”, click “stop”.
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| Click “yes”
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| Save data in two forms (DWA datafile, txt file)
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| Check if the data is properly saved.
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| *If you want to see the reference signals, go to options: view option and display raw R.
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