Biomod/2011/Caltech/DeoxyriboNucleicAwesome/SPEX Results

Thursday, April 18, 2024 Home Members Project Protocols Progress Discussion References

SPEX Results As discussed in SPEX Experimental Design, two sets of experiments were conducted to verify the random walking mechanism. The first set was performed to detect potential leak reactions in the system, while the second set of experiments were used to verify the random walking mechanism. Detection of Potential Leak Reactions To verify that the walker walks on the origami using the intended mechanisms, we set up control groups where either tracks (TR), except for the one that the walker is bound to, or walker triggers (WT), or both, were missing in the reactions. Walkers are not expected to perform random walking under such conditions. As shown in Figure 1, no decrease in fluorescent signals were observed unless both tracks and walker triggers were added into the system. Hence potential leak reactions are negligible. Figure 1. Detection of Potential Leak Reactions. No decrease in fluorescent signals were observed unless both tracks and walker triggers were added into the reaction system. Different fluorescent levels could be attributed to pipetting errors. The gradual increase in fluorescent signals in three control groups was due to increase in fluorophore concentration caused by the evaporation of buffer. Random Walking with Different Starting Positions Data Analysis Matlab Simulation Mathematical Formulae Conclusion