Biomod/2011/HKBU/NBgamers:Results: Difference between revisions

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<h3>Content</h3>
<h3>Contents</h3>
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   <li><a href="#Highly sensitive and high-throughput detection of target molecules">Highly sensitive and high-throughput detection of target molecules</a></li>
   <li></li>
  <li><a href="#Quantification of target molecules">Quantification of target molecules</a></li>
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    <li><a href="#Monoplex detection">Monoplex detection</a></li>
    <li><a href="#Multiplex detection">Multiplex detection</a></li>
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<h3><a name="Highly sensitive and high-throughput detection of target molecules">Highly sensitive and high-throughput detection of target molecules</a></h3>
<p>
Single-stranded nucleic acid targets are captured by complementary nucleic acid probe immobilized on the sensor fibrils as described in the <a href="http://openwetware.org/wiki/Biomod/2011/HKBU/NBgamers:Project#Structure">project structure</a>.
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<p>
To visualize and quantify target sample, a highly selective nucleic acid fluorescence labeling dye <a href="">YOYO-1</a> is utilized. YOYO-1 has high affinity to double-stranded DNS helix over single-stranded. Once it binds to double-stranded, it fluorescence intensity will be enhanced significantly (~1000-fold). In this case, any free YOYO-1 in the solution will be regarded as "silent".
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<p>
As illustrated in the following figure, each fluorescent fibril represents one single sensor. Upon acquisition of one fluorescence image, hundreds of fibrils and thus sensors are visualized and detection simultaneously. This demonstrates the high-throughput manner of our detection assay.
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<h3><a name="Quantification of target molecules">Quantification of target molecules</a></h3>
<p>
Quantification of target is achieved by measuring the resulted YOYO-1 intensity. The concentration of targets should be proportional to the fluorescence intensity emitted by YOYO-1.
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Revision as of 13:35, 2 November 2011

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<tr> <td bgcolor="#fcec00" align="center"> <img src="http://openwetware.org/images/5/53/NBgamers_team_logo.png" width="453" height="143" alt="NBgamers (Team of NanoBiotechnology)" title="NBgamers (Team of NanoBiotechnology)"> </td> </tr>

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<tr> <td bgcolor="#232323" align="center"> <h3 align="center" style="color:white; font-size:20px;" >Results</h3> </td> </tr>

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