Biomod/2011/Harvard/HarvarDNAnos:Designs: Difference between revisions

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=Cargo=
=Cargo=
[[Image:Screen_Shot_2011-10-23_at_1.17.21_PM.png |thumb|right|Figure 4. Strand Displacement Mechanism for Displacing Cargo]]
[[Image:Screen_Shot_2011-10-23_at_1.45.54_PM.png |thumb|right|Figure 5. Photo-cleavable SpacerMechanism for Displacing Cargo]]


With a few container designs in mind, our next goal was to provide them with functionality.   
With a few container designs in mind, our next goal was to provide them with functionality.   
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''[[Biomod/2011/Harvard/HarvarDNAnos:Design_Cargo | Continue reading...]]''
''[[Biomod/2011/Harvard/HarvarDNAnos:Design_Cargo | Continue reading...]]''
See also:
See also:
''[[Biomod/2011/Harvard/HarvarDNAnos:Design_Sphere#Loading Cargo | Cargo in the Sphere]]'', ''[[Biomod/2011/Harvard/HarvarDNAnos:Design_Box | Cargo in the Box]]'', ''[[Biomod/2011/Harvard/HarvarDNAnos:Results#Nanoparticles | Nanoparticle Results]]'', ''[[Biomod/2011/Harvard/HarvarDNAnos:Results#Photocleavage | Photocleavage Results]]''
''[[Biomod/2011/Harvard/HarvarDNAnos:Design_Sphere#Loading Cargo | Cargo in the Sphere]]'', ''[[Biomod/2011/Harvard/HarvarDNAnos:Design_Box | Cargo in the Box]]'', ''[[Biomod/2011/Harvard/HarvarDNAnos:Results#Nanoparticles | Nanoparticle Results]]'', ''[[Biomod/2011/Harvard/HarvarDNAnos:Results#Photocleavage | Photocleavage Results]]''

Revision as of 19:07, 28 October 2011

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Home              Mission              Process              Designs              Results              Resources              Team

Rectangular Box Container Design

Figure 1. A model of a component of our rectangular box.

Andersen's box impressed us with its ability to open and close, but we worried about its robustness and tightness as a container.

  • Cryo-EM imaging performed by Andersen revealed that the faces are either bent inward or outward.
  • Furthermore, the Andersen box is formed from a one-layer DNA sheet and, as such, is held together by five potentially weak seams.

Therefore, with the help of Wei Sun, we have designed our own box, which we feel stands a much better chance of keeping cargo inside and which is more straightforward to fold and to characterize.

Continue reading...

See also: Rectangular Box Results, Rectangular Box Methods

Spherical Container Design

Figure 2. A three-dimensional model of the Han sphere (Han et al. 2011).

In our search for a robust and elegant design, we were inspired by the origami sphere that Dongran Han demonstrated in his 2011 Science paper "DNA Origami with Complex Curvatures in Three-Dimensional Space". The design principles for an origami sphere (and other 3D origami with complex curvatures) employed by Han are the following (see Figure 1):

  • Multi-planar arrangement of parallel double helices with in-plane curvature of helices into rings, and
  • Curvature across planes caused by different ring sizes and greater distance between crossovers in larger rings than in smaller rings.

Continue reading...

See also: Sphere Results, Sphere Methods

Cargo

With a few container designs in mind, our next goal was to provide them with functionality.

  • We decided to use 5 nm gold nanoparticle cargo as a test platform for our ability to capture, contain, and controllably release cargo.
  • We decided to use 5 nm gold nanoparticles because the sharp contrast they provide under TEM would help us to classify our results easily.

Continue reading... See also: Cargo in the Sphere, Cargo in the Box, Nanoparticle Results, Photocleavage Results



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