Biomod/2011/Harvard/HarvarDNAnos:Designs: Difference between revisions
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=Rectangular Box Container Design= | =Rectangular Box Container Design= |
Revision as of 21:55, 30 October 2011
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Rectangular Box Container Design
Andersen's box impressed us with its ability to open and close, but we worried about its robustness and tightness as a container.
- Cryo-EM imaging performed by Andersen revealed that the faces are either bent inward or outward.
- Furthermore, the Andersen box is formed from a one-layer DNA sheet and, as such, is held together by five potentially weak seams.
Therefore, with the help of Wei Sun, we have designed our own box, which we feel stands a much better chance of keeping cargo inside and which is more straightforward to fold and to characterize.
See also: Rectangular Box Results, Rectangular Box Methods
Spherical Container Design
In our search for a robust and elegant design, we were inspired by the origami sphere that Dongran Han demonstrated in his 2011 Science paper "DNA Origami with Complex Curvatures in Three-Dimensional Space" (Figure 2).
The spherical design appealed to us because of its efficient use of DNA and lack of weak points--that is, instead of having edges, it only has two holes at each pole, minimizing spots where cargo can leak out. We imagined that we would be able to change the design of the Han sphere to make it an openable and closable container.
See also: Sphere Results, Sphere Methods
Cargo Design
With a few container designs in mind, our next goal was to provide them with functionality.
- We use 5-nm gold nanoparticle cargo as a test platform for our ability to capture, contain, and controllably release cargo.
- We chose 5-nm gold nanoparticles because the sharp contrast they provide under TEM helps us to classify our results easily.
See also: Cargo in the Sphere, Cargo in the Box, Nanoparticle Results, Photocleavage Results