Biomod/2011/Harvard/HarvarDNAnos:Results: Difference between revisions

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{{Template:Biomod/2011/Harvard/HarvardDNAnos}}
{{Template:Biomod/2011/Harvard/HarvardDNAnos}}


==Overview==
=Results Overview=
'''We have accomplished the following:'''
'''We have accomplished the following:'''
*'''With our original rectangular box design, we have developed a system that can trap, hold, and release nanocargo.'''
*'''With our original rectangular box design, we have developed a system that can trap, hold, and release nanocargo.'''


*'''We have also opened Han’s sphere in preparation for future nanocargo encapsulation.'''
*'''We have opened Han’s sphere in preparation for future nanocargo encapsulation.'''


*'''We are now one step closer to achieving simpler signal amplification, more complex logic gates, and submarine-like drug delivery.'''
*'''We are now one step closer to achieving simpler signal amplification, more complex logic gates, and submarine-like drug delivery.'''


==Box Container==
=Box Container=
[[Image:Barrelgoldlidgold2.tif|thumb|Figure 1. Caption]]
[[Image:Barrelgoldlidgold2.tif|thumb|150px|Figure 1. A box with enclosed gold under a transmission electron microscope.]]
*We have designed and folded two robust origami structures: the lid and the barrel. These two structures are the components of our box.
*We designed and folded two robust origami structures: the lid and the barrel. These two structures are the components of our box.
*We demonstrate that we can close and open the box with high efficiency.
*We demonstrated that we can close and open the box with high efficiency.
*We demonstrate that we can attach AuNP cargo to our lids and/or our barrels and then close the box, placing the AuNP cargo within the box.
*We demonstrated that we can attach AuNP cargo to our lids and/or our barrels and then close the box, placing the AuNP cargo within the box.
*We demonstrate that we can photocleave the AuNP cargo off of our lids, solubilizing the cargo.
*We demonstrated that we can photocleave the AuNP cargo off of our lids, solubilizing the cargo.
 


[[Biomod/2011/Harvard/HarvarDNAnos:Results_Box | ''Continue reading...'']]
[[Biomod/2011/Harvard/HarvarDNAnos:Results_Box | ''Continue reading...'']]
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''[[Biomod/2011/Harvard/HarvarDNAnos:Methods#Rectangular Box Design | Rectangular Box Methods]]''
''[[Biomod/2011/Harvard/HarvarDNAnos:Methods#Rectangular Box Design | Rectangular Box Methods]]''


==Spherical Container==
=Spherical Container=
[[Image:2011-08-10_spheregelpur8bp_bottom1.jpg|thumb|150px|right| Figure 2. Open spheres under an atomic force microscope.]]
With our sphere design, we were able to:
*Fold and characterize through atomic force microscopy (AFM) the original Han et al. sphere, which we call the "closed" sphere.
*Fold and characterize through AFM our "open" sphere in which we removed all equator staple strands.
*Test various lock mechanisms to transition between the closed and open states of the sphere.
*Analyze the scaffold-staple-lock system to find out why certain lock designs were unsuccessful at closing the sphere.
 
 
[[Biomod/2011/Harvard/HarvarDNAnos:Results_Sphere | ''Continue reading...'']]
[[Biomod/2011/Harvard/HarvarDNAnos:Results_Sphere | ''Continue reading...'']]


==Nanoparticles==
See also: ''[[Biomod/2011/Harvard/HarvarDNAnos:Design_Sphere | Sphere Design]]'',
We successfully:
''[[Biomod/2011/Harvard/HarvarDNAnos:Methods#Sphere Methods| Sphere Methods]]''
* synthesized 5nm gold particles and conjugated DNA to their surfaces
 
* conjugated DNA to commercially available 5nm gold nanoparticles
=Nanoparticles=
* integrated AuNP-DNA conjugates into our rectangular box design  
[[Image:Screen_Shot_2011-07-27_at_3.32.34_PM.png|thumb|right|150px| Figure 3. Evidence of DNA conjugation to gold nanoparticles]]
[[Biomod/2011/Harvard/HarvarDNAnos:Results_AuNP | ''Continue reading...'']]
*We synthesized 5nm gold particles and conjugated DNA to their surfaces
*We conjugated DNA to commercially available 5 nm gold nanoparticles
*We integrated AuNP-DNA conjugates into our rectangular box design  
<br>[[Biomod/2011/Harvard/HarvarDNAnos:Results_AuNP | ''Continue reading...'']]
<br>See also: ''[[Biomod/2011/Harvard/HarvarDNAnos:Design_Cargo | Cargo Design]]''


==Photo-cleavage==
=Photo-cleavage=
* We have demonstrated photo-cleavage of strands with internal photo-cleavable spacers
* We demonstrated photo-cleavage of strands with internal photo-cleavable spacers
* We have outlined a process for the use of photo-cleavable spacers as a means to open our containers and solubilize our cargo  
* We outlined a process for the use of photo-cleavable spacers as a means to open our containers and solubilize our cargo  


[[Biomod/2011/Harvard/HarvarDNAnos:Results_PC | ''Continue reading...'']]
<br>[[Biomod/2011/Harvard/HarvarDNAnos:Results_PC | ''Continue reading...'']]
<br>See also: ''[[Biomod/2011/Harvard/HarvarDNAnos:Design_Cargo | Cargo Design]]''


==Tools and Parts==
=Tools and Parts=
*[[Biomod/2011/Harvard/HarvarDNAnos:SphereCAD | SphereCAD]]
*[[Biomod/2011/Harvard/HarvarDNAnos:SphereCAD | SphereCAD]]
*[[Biomod/2011/Harvard/HarvarDNAnos:caDNAnoFiles | Repository of caDNAno Files]]  
*[[Biomod/2011/Harvard/HarvarDNAnos:caDNAnoFiles | Repository of caDNAno Files]]  

Latest revision as of 21:42, 2 November 2011

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Home              Mission              Process              Designs              Results              Resources              Team

Results Overview

We have accomplished the following:

  • With our original rectangular box design, we have developed a system that can trap, hold, and release nanocargo.
  • We have opened Han’s sphere in preparation for future nanocargo encapsulation.
  • We are now one step closer to achieving simpler signal amplification, more complex logic gates, and submarine-like drug delivery.

Box Container

Figure 1. A box with enclosed gold under a transmission electron microscope.
  • We designed and folded two robust origami structures: the lid and the barrel. These two structures are the components of our box.
  • We demonstrated that we can close and open the box with high efficiency.
  • We demonstrated that we can attach AuNP cargo to our lids and/or our barrels and then close the box, placing the AuNP cargo within the box.
  • We demonstrated that we can photocleave the AuNP cargo off of our lids, solubilizing the cargo.


Continue reading...

See also: Rectangular Box Design, Rectangular Box Methods

Spherical Container

Figure 2. Open spheres under an atomic force microscope.

With our sphere design, we were able to:

  • Fold and characterize through atomic force microscopy (AFM) the original Han et al. sphere, which we call the "closed" sphere.
  • Fold and characterize through AFM our "open" sphere in which we removed all equator staple strands.
  • Test various lock mechanisms to transition between the closed and open states of the sphere.
  • Analyze the scaffold-staple-lock system to find out why certain lock designs were unsuccessful at closing the sphere.


Continue reading...

See also: Sphere Design, Sphere Methods

Nanoparticles

Figure 3. Evidence of DNA conjugation to gold nanoparticles
  • We synthesized 5nm gold particles and conjugated DNA to their surfaces
  • We conjugated DNA to commercially available 5 nm gold nanoparticles
  • We integrated AuNP-DNA conjugates into our rectangular box design


Continue reading...
See also: Cargo Design

Photo-cleavage

  • We demonstrated photo-cleavage of strands with internal photo-cleavable spacers
  • We outlined a process for the use of photo-cleavable spacers as a means to open our containers and solubilize our cargo


Continue reading...
See also: Cargo Design

Tools and Parts


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