Biomod/2011/Harvard/HarvarDNAnos:Results

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Home              Mission              Process              Designs              Results              Resources              Team


Contents

Results Overview

We have accomplished the following:

  • With our original rectangular box design, we have developed a system that can trap, hold, and release nanocargo.
  • We have opened Han’s sphere in preparation for future nanocargo encapsulation.
  • We are now one step closer to achieving simpler signal amplification, more complex logic gates, and submarine-like drug delivery.

Box Container

Figure 1. A box with enclosed gold under a transmission electron microscope.
Figure 1. A box with enclosed gold under a transmission electron microscope.
  • We designed and folded two robust origami structures: the lid and the barrel. These two structures are the components of our box.
  • We demonstrated that we can close and open the box with high efficiency.
  • We demonstrated that we can attach AuNP cargo to our lids and/or our barrels and then close the box, placing the AuNP cargo within the box.
  • We demonstrated that we can photocleave the AuNP cargo off of our lids, solubilizing the cargo.


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See also: Rectangular Box Design, Rectangular Box Methods

Spherical Container

Figure 2. Open spheres under an atomic force microscope.
Figure 2. Open spheres under an atomic force microscope.

With our sphere design, we were able to:

  • Fold and characterize through atomic force microscopy (AFM) the original Han et al. sphere, which we call the "closed" sphere.
  • Fold and characterize through AFM our "open" sphere in which we removed all equator staple strands.
  • Test various lock mechanisms to transition between the closed and open states of the sphere.
  • Analyze the scaffold-staple-lock system to find out why certain lock designs were unsuccessful at closing the sphere.


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See also: Sphere Design, Sphere Methods

Nanoparticles

Figure 3. Evidence of DNA conjugation to gold nanoparticles
Figure 3. Evidence of DNA conjugation to gold nanoparticles
  • We synthesized 5nm gold particles and conjugated DNA to their surfaces
  • We conjugated DNA to commercially available 5 nm gold nanoparticles
  • We integrated AuNP-DNA conjugates into our rectangular box design


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See also: Cargo Design

Photo-cleavage

  • We demonstrated photo-cleavage of strands with internal photo-cleavable spacers
  • We outlined a process for the use of photo-cleavable spacers as a means to open our containers and solubilize our cargo


Continue reading...
See also: Cargo Design

Tools and Parts


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