Biomod/2011/TUM/TNT/LabbookA/2011/09/01: Difference between revisions

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==Real time PCR mit MH255 und MH256 gelabelt mit Atto 550 und 647 N unter Zugabe von EtBr und Spermin==
==Real time PCR with MH255_647 and MH256_550 adding ErBr and Spermine==
*  
* MH255_647 and MH256_550 are oligonucleotides of 18 nucleotides each, labeled with Atto fluorescent dyes Atto 647 and Atto 550.
* The DNA intercalator Ethidiumbromide and the major groove binder Spermine are used at various concentrations accoring to their Kd-Values
*





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Real time PCR with MH255_647 and MH256_550 adding ErBr and Spermine

  • MH255_647 and MH256_550 are oligonucleotides of 18 nucleotides each, labeled with Atto fluorescent dyes Atto 647 and Atto 550.
  • The DNA intercalator Ethidiumbromide and the major groove binder Spermine are used at various concentrations accoring to their Kd-Values