Biomod/2011/TUM/TNT/LabbookA/2011/09/01: Difference between revisions

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* The K<sub>D</sub> value of Spermin is 4,8µL and that of ethidium bromide is 12µL.
* The K<sub>D</sub> value of Spermin is 4,8µL and that of ethidium bromide is 12µL.
* The concentrations of Spermin and ethidium bromide are as follows:
* The concentrations of Spermin and ethidium bromide are as follows:
1/19*K<sub>D</sub>, 1/9*K<sub>D</sub>, 1/4*K<sub>D</sub>, 1/2*K<sub>D</sub>, 1*K<sub>D</sub>, 2*K<sub>D</sub>, 4*K<sub>D</sub>, 9*K<sub>D</sub>, 19*K<sub>D</sub>.
**1/19*K<sub>D</sub>, 1/9*K<sub>D</sub>, 1/4*K<sub>D</sub>, 1/2*K<sub>D</sub>, 1*K<sub>D</sub>, 2*K<sub>D</sub>, 4*K<sub>D</sub>, 9*K<sub>D</sub>, 19*K<sub>D</sub>.




Revision as of 03:54, 2 September 2011

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Real time PCR with MH255_647 and MH256_550 adding ErBr and Spermine

  • MH255_647 and MH256_550 are oligonucleotides of 18 nucleotides each, labeled with Atto fluorescent dyes Atto 647 and Atto 550.
  • The DNA intercalator ethidium bromide and the major groove binder Spermine are added at various concentrations accoring to their KD-Values.
  • The KD value of Spermin is 4,8µL and that of ethidium bromide is 12µL.
  • The concentrations of Spermin and ethidium bromide are as follows:
    • 1/19*KD, 1/9*KD, 1/4*KD, 1/2*KD, 1*KD, 2*KD, 4*KD, 9*KD, 19*KD.



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