Biomod/2011/TUM/TNT/LabbookA/2011/09/01: Difference between revisions
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**Spermin: 50µM and 200µM | **Spermin: 50µM and 200µM | ||
**EtBr: 50µM and 200µM | **EtBr: 50µM and 200µM | ||
*Concentrations of DNA | *Concentrations of DNA solutions: | ||
**MH255_647N: 2,8µM | **MH255_647N: 2,8µM | ||
**MH256_550: 7,1µM | **MH256_550: 7,1µM | ||
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! concentration spermine | ! concentration spermine | ||
! Spermin Stock [µL] | ! Spermin Stock [µL] | ||
! MH255_647[µL] | ! MH255_647 0,28µM [µL] | ||
! MH256_550[µL] | ! MH256_550 0,71µM [µL] | ||
! buffer (0,5 TBE, 11mM MgCl<sub>2</sub>) | ! buffer (0,5 TBE, 11mM MgCl<sub>2</sub>) | ||
|- | |- | ||
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! concentration spermine | ! concentration spermine | ||
! Spermin Stock [µL] | ! Spermin Stock [µL] | ||
! MH255[µL] | ! MH255 1µM [µL] | ||
! MH256[µL] | ! MH256 1µM [µL] | ||
! buffer (0,5 TBE, 11mM MgCl<sub>2</sub>) | ! buffer (0,5 TBE, 11mM MgCl<sub>2</sub>) | ||
|- | |- | ||
Line 167: | Line 167: | ||
! concentration EtBr | ! concentration EtBr | ||
! EtBr Stock [µL] | ! EtBr Stock [µL] | ||
! MH255_647[µL] | ! MH255_647 0,28µM [µL] | ||
! MH256_550[µL] | ! MH256_550 0,71µM [µL] | ||
! buffer (0,5 TBE, 11mM MgCl<sub>2</sub>) | ! buffer (0,5 TBE, 11mM MgCl<sub>2</sub>) | ||
|- | |- | ||
Line 232: | Line 232: | ||
! concentration EtBr | ! concentration EtBr | ||
! EtBr Stock [µL] | ! EtBr Stock [µL] | ||
! MH255[µL] | ! MH255 1µM [µL] | ||
! MH256[µL] | ! MH256 1µM [µL] | ||
! buffer (0,5 TBE, 11mM MgCl<sub>2</sub>) | ! buffer (0,5 TBE, 11mM MgCl<sub>2</sub>) | ||
|- | |- |
Revision as of 07:22, 2 September 2011
Project name | <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page <html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html> </html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html> | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Real time PCR with MH255_647 and MH256_550 adding ErBr and Spermine
-> dilution 1:10 in buffer is used for RtPCR
-> dilution 1:100 in buffer is used for RtPCR Pipeting scheme spermine:
Pipeting scheme spermine control without labels: Error: there was added 1 µL of buffer too much
Pipeting scheme ethidium bromide control without labels: Error: there was added 1 µL of buffer too much
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