Biomod/2011/TUM/TNT/LabbookA/2011/10/13: Difference between revisions

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===Preparation===
===Preparation===
* Preparation was done according to [http://openwetware.org/wiki/Biomod/2011/TUM/TNT/LabbookA/Immobilizing_structures_with_biotin-oligos_for_TIRF Immobilizing structures with biotin oligos for TIRF]
* Preparation was done according to [http://openwetware.org/wiki/Biomod/2011/TUM/TNT/LabbookA/Immobilizing_structures_with_biotin-oligos_for_TIRF Immobilizing structures with biotin oligos for TIRF]
* The spermine concentrations were calculated acoording to
* The spermine concentrations were set to  
** one molecule spermine per 7 bases (= 0.802 μM)
** one molecule spermine per 21 bases (= 0.241 μM)
** no spermine
 
For calculations see [http://openwetware.org/wiki/Biomod/2011/TUM/TNT/LabbookA/Calculation_of_intercalator_concentrations Calculation of intercalator concentrations].




Revision as of 06:02, 17 October 2011

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Single molecule fluorescence microscopy measurement of BM21_172/173 with/without spermine

Aim

  • Seeing tendencies in changes in length of the origami when the intercalator spermine is added.

Preparation

For calculations see Calculation of intercalator concentrations.


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