Biomod/2011/TUM/TNT/LabbookA/2011/10/18: Difference between revisions
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==Fluorescence microscope slides of [http://openwetware.org/wiki/Biomod/2011/TUM/TNT/LabbookA/Structure_page#BM12_to_BM14:_Fluorophore-labeled_structures_for_FRET_measurements.2C_including_adapters_for_immobilisation BM12_4/29]== | ==Fluorescence microscope slides of [http://openwetware.org/wiki/Biomod/2011/TUM/TNT/LabbookA/Structure_page#BM12_to_BM14:_Fluorophore-labeled_structures_for_FRET_measurements.2C_including_adapters_for_immobilisation BM12_4/29]== | ||
* In order to measure the spatial separation of the two | * In order to measure the spatial separation of the two fluorophores, we decided to take pictures with red and green illumination (each picture is separately illuminated with its color). From this distance we want to evaluate the twist of the arms | ||
* therefore we took the BM 12_4/29 structure, in which ATTO 550 is incorporated in helix 4 and ATTO 647N in helix 29 | * therefore we took the BM 12_4/29 structure, in which ATTO 550 is incorporated in helix 4 and ATTO 647N in helix 29 | ||
* pipet plan for the concentration of DNA binder solution: [[Image:20111018_BM12_4_29_pipettplan.xlsx]] | * pipet plan for the concentration of DNA binder solution: [[Image:20111018_BM12_4_29_pipettplan.xlsx]] | ||
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* prepared microscope slides as indicated in the wiki: [http://openwetware.org/wiki/Biomod/2011/TUM/TNT/LabbookA/Immobilizing_structures_with_biotin-oligos_for_TIRF Immobilizing structures with biotin-oligos for TIRF] | * prepared microscope slides as indicated in the wiki: [http://openwetware.org/wiki/Biomod/2011/TUM/TNT/LabbookA/Immobilizing_structures_with_biotin-oligos_for_TIRF Immobilizing structures with biotin-oligos for TIRF] | ||
* BM12_4/29 was diluted 1 to 5000 with FOB20 | * BM12_4/29 was diluted 1 to 5000 with FOB20 | ||
===Measurement=== | |||
From each sample, 100 picture pairs (green and red) were made. | |||
</div> | </div> |
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18th Oct 2011
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Main project page
labeled oligonucleotides for twisted structure BM25according to standard procedures Fluorescence microscope slides of BM12_4/29
BM12_Spermine_1to7
BM12_Spermine_1to21
MeasurementFrom each sample, 100 picture pairs (green and red) were made.
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