Biomod/2011/TUM/TNT/LabbookA/2011/Dyes: Difference between revisions

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19. oct Camera calibration<br>
19. oct Camera calibration<br>
<br>
<br>
<h2>Bidner</h2>
<h2>Binder</h2>
<br>
<br>
11. aug Measured absorption and emission spectra of Atto 550 and Atto 647N together with DNA binders<br>
11. aug Measured absorption and emission spectra of Atto 550 and Atto 647N together with DNA binders<br>

Revision as of 10:01, 1 November 2011

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Dyes and DNA-binders

http://openwetware.org/index.php?title=Biomod/2011/TUM/TNT/LabbookA/2011/Dyes&action=edit

Structure

23. aug Folding first structures: The U
24. aug Purification of the U structure from 15_65 thermal ramp by agarose gel electrophoresis
29. aug Purification of BM1 and BM2 folded with 2DH3ML and 5DH3ML via agarose gel electrophoresis
2. sept Folding fluorophore labeled the U structures
6. sept Size exclusion filter purification of BM_2_15_65 and BM_2_5D_H3_ML
6. sept Size exclusion filter purification of folded BM_1_15_65
12. sept Size exclusion filter purification
27. sept Folding fluorophore labeled structures with adapter staples for immobilisation
29. sept purified origami structures from 27th September
17. oct folded BM24
19.oct determined concentrations of labeled oligonucleotides
19. oct folded structure BM25_4/29

TIRF


13. sept Fluorescence microscopic ensemble measurement of BM5_5/20
14. sept Samples for TIRF bulk measurements with spermine
15. sept Fluorescence microscope bulk measurements with spermine
16. sept Fluorescence microscopic measurements from 2011/09/15
29. sept preparing samples for single molecule fluorescence microscopy
30. sept Single molecule fluorescence microscopy measurement of BM14_5/20 and BM21_172/173
6. oct single molecule fluorescence microscopy with sample BM21_172/173
13. oct Single molecule fluorescence microscopy measurement of BM21_172/173 with/without spermine
18. oct Fluorescence microscope slides of BM12_4/29
19. oct Camera calibration

Binder


11. aug Measured absorption and emission spectra of Atto 550 and Atto 647N together with DNA binders
17. aug Measuring the absorption coefficients and concentrations of single stranded labled DNA
23. aug Preparing DAPI concentration series for the helix MH255/256
24. aug Labeling oligos with fluorophores
25. aug Determined Concentrations of Labeled Oligos
30. aug Measurement of DAPI concentration series for the helix MH255/256 in Real Time PCR
1.sept Real time PCR with MH_255_647N and MH_256_550 adding ethidium bromide and spermine
6. sept Photometric spectroscopy of MH_255_647 and MH_256_550 with EtBr
19. sept Labeled oligonucleotides
19. sept Determination of absorption coefficient of unlabeled the_U_172 and the_U_173
20. sept Measured concentrations of labeled oligonucleotides
18. oct labeled oligonucleotides for twisted structure BM25

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