Biomod/2011/TUM/TNT/LabbookA/list TEM pictures: Difference between revisions
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<tr bgcolor="#f5f5f5"><td>A1</td><td>oct. 5th</td><td>BM2 1:20 with 1.34 µM spermine (corresponding to one molecule every 7bp)</td><td> | <tr bgcolor="#f5f5f5"><td>A1</td><td>oct. 5th</td><td>BM2 1:20 with 1.34 µM spermine (corresponding to one molecule every 7bp)</td><td>oct. 7th</td><td>good stain, slightly to high structure concentration, 60 images</td></tr> | ||
<tr bgcolor="#f5f5f5"><td>A2</td><td>oct. 5th</td><td>BM2 1:20 with 1.34 µM spermine (corresponding to one molecule every 7bp)</td><td> | <tr bgcolor="#f5f5f5"><td>A2</td><td>oct. 5th</td><td>BM2 1:20 with 1.34 µM spermine (corresponding to one molecule every 7bp)</td><td>oct. 7th</td><td>good stain, slightly to high structure concentration, 60 images</td></tr> | ||
<tr bgcolor="#f5f5f5"><td>A3</td><td>oct. 5th</td><td>BM2 1:20 with 1.34 µM spermine (corresponding to one molecule every 7bp)</td><td> | <tr bgcolor="#f5f5f5"><td>A3</td><td>oct. 5th</td><td>BM2 1:20 with 1.34 µM spermine (corresponding to one molecule every 7bp)</td><td>oct. 7th</td><td>good stain, slightly to high structure concentration, 60 images</td></tr> | ||
<tr bgcolor="#f5f5f5"><td>A4</td><td>oct. 5th</td><td>BM2 1:20 with 0.42 µM spermine (corresponding to one molecule every 21bp)</td><td> | <tr bgcolor="#f5f5f5"><td>A4</td><td>oct. 5th</td><td>BM2 1:20 with 0.42 µM spermine (corresponding to one molecule every 21bp)</td><td>oct. 7th</td><td>good stain, slightly to high structure concentration, 60 images</td></tr> | ||
<tr bgcolor="#f5f5f5"><td>A5</td><td>oct. 5th</td><td>BM2 1:20 with 0.42 µM spermine (corresponding to one molecule every 21bp)</td><td> | <tr bgcolor="#f5f5f5"><td>A5</td><td>oct. 5th</td><td>BM2 1:20 with 0.42 µM spermine (corresponding to one molecule every 21bp)</td><td>oct. 7th</td><td>good stain, slightly to high structure concentration, 60 images</td></tr> | ||
<tr bgcolor="#f5f5f5"><td>A6</td><td>oct. 20th</td><td>BM2 with 432 nM DAPI (corresponding to one molecule every 7bp), 1:25</td><td>oct. 28st</td><td>no origamis on the grid</td></tr> | <tr bgcolor="#f5f5f5"><td>A6</td><td>oct. 20th</td><td>BM2 with 432 nM DAPI (corresponding to one molecule every 7bp), 1:25</td><td>oct. 28st</td><td>no origamis on the grid</td></tr> | ||
<tr bgcolor="#f5f5f5"><td>A7</td><td>oct. 20th</td><td>BM2 with 144 nM DAPI (corresponding to one molecule every 21bp), 1:25</td><td>oct. 27th</td><td>no origamis on the grid</td></tr> | <tr bgcolor="#f5f5f5"><td>A7</td><td>oct. 20th</td><td>BM2 with 144 nM DAPI (corresponding to one molecule every 21bp), 1:25</td><td>oct. 27th</td><td>no origamis on the grid</td></tr> | ||
Revision as of 06:33, 30 October 2011
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http://openwetware.org/index.php?title=Biomod/2011/TUM/TNT/LabbookA/list_TEM_pictures&action=edit
summary of all prepared and observed grids
All dilutions were made with FOB20. For the calculation of the intercalator concentration read more in the corresponding labbook entry.
BIOMOD1 grid box
| grid number | prepared | annotations | observed | annotation |
| A1 | 2 | 3 | 4 | 5 |
| A2 | 2 | 3 | 4 | 5 |
| A3 | 2 | 3 | 4 | 5 |
| A4 | 2 | 3 | 4 | 5 |
| A5 | 2 | 3 | 4 | 5 |
| A6 | 2 | 3 | 4 | 5 |
| A7 | 2 | 3 | 4 | 5 |
| A8 | 2 | 3 | 4 | 5 |
| A9 | 2 | 3 | 4 | 5 |
| A10 | 2 | 3 | 4 | 5 |
| B1 | 2 | 3 | 4 | 5 |
| B2 | 2 | 3 | 4 | 5 |
| B3 | 2 | 3 | 4 | 5 |
| B4 | 2 | 3 | 4 | 5 |
| B5 | 2 | 3 | 4 | 5 |
| B6 | 2 | 3 | 4 | 5 |
| B7 | 2 | 3 | 4 | 5 |
| B8 | 2 | 3 | 4 | 5 |
| B9 | 2 | 3 | 4 | 5 |
| B10 | 2 | 3 | 4 | 5 |
| C1 | 2 | 3 | 4 | 5 |
| C2 | 2 | 3 | 4 | 5 |
| C3 | 2 | 3 | 4 | 5 |
| C4 | 2 | 3 | 4 | 5 |
| C5 | 2 | 3 | 4 | 5 |
| C6 | 2 | 3 | 4 | 5 |
| C7 | 2 | 3 | 4 | 5 |
| C8 | 2 | 3 | 4 | 5 |
| C9 | 2 | 3 | 4 | 5 |
| C10 | 2 | 3 | 4 | 5 |
| D1 | 2 | 3 | 4 | 5 |
| D2 | 2 | 3 | 4 | 5 |
| D3 | 2 | 3 | 4 | 5 |
| D4 | 2 | 3 | 4 | 5 |
| D5 | 2 | 3 | 4 | 5 |
| D6 | 2 | 3 | 4 | 5 |
| D7 | 2 | 3 | 4 | 5 |
| D8 | 2 | 3 | 4 | 5 |
| D9 | 2 | 3 | 4 | 5 |
| D10 | 2 | 3 | 4 | 5 |
| E1 | 2 | 3 | 4 | 5 |
| E2 | 2 | 3 | 4 | 5 |
| E3 | 2 | 3 | 4 | 5 |
| E4 | 2 | 3 | 4 | 5 |
| E5 | 2 | 3 | 4 | 5 |
| E6 | 2 | 3 | 4 | 5 |
| E7 | 2 | 3 | 4 | 5 |
| E8 | 2 | 3 | 4 | 5 |
| E9 | 2 | 3 | 4 | 5 |
| E10 | 2 | 3 | 4 | 5 |
BIOMOD2 grid box
| grid number | observed | annotations | observed | annotation |
| A1 | oct. 5th | BM2 1:20 with 1.34 µM spermine (corresponding to one molecule every 7bp) | oct. 7th | good stain, slightly to high structure concentration, 60 images |
| A2 | oct. 5th | BM2 1:20 with 1.34 µM spermine (corresponding to one molecule every 7bp) | oct. 7th | good stain, slightly to high structure concentration, 60 images |
| A3 | oct. 5th | BM2 1:20 with 1.34 µM spermine (corresponding to one molecule every 7bp) | oct. 7th | good stain, slightly to high structure concentration, 60 images |
| A4 | oct. 5th | BM2 1:20 with 0.42 µM spermine (corresponding to one molecule every 21bp) | oct. 7th | good stain, slightly to high structure concentration, 60 images |
| A5 | oct. 5th | BM2 1:20 with 0.42 µM spermine (corresponding to one molecule every 21bp) | oct. 7th | good stain, slightly to high structure concentration, 60 images |
| A6 | oct. 20th | BM2 with 432 nM DAPI (corresponding to one molecule every 7bp), 1:25 | oct. 28st | no origamis on the grid |
| A7 | oct. 20th | BM2 with 144 nM DAPI (corresponding to one molecule every 21bp), 1:25 | oct. 27th | no origamis on the grid |
| A8 | oct. 20th | BM2 with 144 nM DAPI (corresponding to one molecule every 21bp), 1:25 | oct. 28th | no origamis on the grid |
| A9 | oct. 20th | BM2 without any DNA binders, 1:25 | 4 | 5 |
| A10 | oct. 20th | BM2 without any DNA binders, 1:25 | 4 | 5 |
| B1 | oct. 5th | BM2 1:20 with 0.42 µM spermine (corresponding to one molecule every 21bp) | 4 | 5 |
| B2 | oct. 10th | BM2 1:40 with 2.27 µM ethidium bromide (corresponding to one molecule every 7bp) | 4 | 5 |
| B3 | oct. 10th | BM2 1:40 with 2.27 µM ethidium bromide (corresponding to one molecule every 7bp) | 4 | 5 |
| B4 | oct. 10th | BM2 1:40 with 2.27 µM ethidium bromide (corresponding to one molecule every 7bp) | 4 | 5 |
| B5 | oct. 10th | BM2 1:40 with 0.69 µM ethidium bromide (corresponding to one molecule every 21bp) | 4 | 5 |
| B6 | oct. 20th | BM2 without any DNA binders, 1:25 | 4 | 5 |
| B7 | oct. 20th | BM2 without any DNA binders, 1:25 | 4 | 5 |
| B8 | oct. 20th | BM24 15_65 1:25 without any DNA binders | 4 | 5 |
| B9 | oct. 20th | BM24 2DH3ML 1:25 without any DNA binders | 4 | 5 |
| B10 | oct. 21th | BM24 15_65 1:20 without any DNA binders | 4 | 5 |
| C1 | oct. 10th | BM2 1:40 with 0.69 µM ethidium bromide (corresponding to one molecule every 21bp) | 4 | 5 |
| C2 | oct. 10th | BM2 1:40 with 0.69 µM ethidium bromide (corresponding to one molecule every 21bp) | 4 | 5 |
| C3 | oct. 10th | BM2 1:40 without any DNA binders | 4 | 5 |
| C4 | oct. 10th | BM2 1:40 without any DNA binders | 4 | 5 |
| C5 | oct. 10th | BM2 1:40 without any DNA binders | 4 | 5 |
| C6 | oct. 21th | BM24 15_65 dilution 1:20 without any DNA binders | oct. 27th | fine grid, 60 images |
| C7 | oct. 21th | BM24 2DH3ML dilution 1:20 without any DNA binders | 4 | 5 |
| C8 | oct. 21th | BM24 2DH3ML 1:20 without any DNA binders | 4 | 5 |
| C9 | oct. 27th | BM2 1:25 without any DNA binders | 4 | 5 |
| C10 | oct. 27th | BM2 1:25 without any DNA binders | oct. 27th | fine grid, 60 images |
| D1 | oct. 11th | BM2 1:25 with 2.4 µM ethidium bromide (corresponding to one molecule every 7bp) | 4 | 5 |
| D2 | oct. 11th | BM2 1:25 with 0.74 µM ethidium bromide (corresponding to one molecule every 21bp) | 4 | 5 |
| D3 | oct. 11th | BM2 1:25without any DNA binders | 4 | 5 |
| D4 | oct. 14th | BM2 1:25 with 432 µM DAPI (corresponding to one molecule every 7bp) | 4 | 5 |
| D5 | oct. 14th | BM2 1:25 with 432 nM DAPI (corresponding to one molecule every 7bp) | oct. 27th | low sample concentration |
| D6 | oct. 27th | BM24 (15_65), 1:20 diluted with FOB20 | 4 | 5 |
| D7 | oct. 27th | lost the grid before sample preparation | 4 | 5 |
| D8 | oct. 27th | BM24 (15_65) 1:10 without any DNA binders | oct. 27th | low sample concentration, 24 images |
| D9 | oct. 27th | BM24 (15_65) 1:10 without any DNA binders | 4 | 5 |
| D10 | oct. 28st | BM24 (15_65) 1:5 without any DNA binders | oct. 28st | better sample concentration than 2D6 and 2D8, 40 images |
| E1 | oct. 20th | BM2 1:25 with 2.4 µM EtBr (corresponding to one molecule every 7bp) | oct. 26th | good dilution, bad staining |
| E2 | oct. 20th | BM2 1:25 with 2.4 µM EtBr (corresponding to one molecule every 7bp) | oct. 26th | strong background |
| E3 | oct. 20th | BM2 1:25 with 0.74 µM EtBr (corresponding to one molecule every 21bp) | oct. 26th | fine grid |
| E4 | oct. 20th | BM2 1:25 with 0.74 µM EtBr (corresponding to one molecule every 21bp) | oct. 26th | fine grid |
| E5 | oct. 20th | BM2 1:25 with 432 nM DAPI (corresponding to one molecule every 7bp) | oct. 26th | weak stain |
| E6 | oct. 28st | BM24 (15_65), 1:5 | oct. 28st | fine grid, 100 images |
| E7 | 2 | 3 | 4 | 5 |
| E8 | 2 | 3 | 4 | 5 |
| E9 | 2 | 3 | 4 | 5 |
| E10 | 2 | 3 | 4 | 5 |
