Biomod/2011/TUM/TNT/LabbookA/list TEM pictures: Difference between revisions
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<tr bgcolor="#f5f5f5"><td>B2</td><td>sep. 16th</td><td> | <tr bgcolor="#f5f5f5"><td>B2</td><td>sep. 16th</td><td>BM2 1:5 control without DNA-binders</td><td>sep. 21th</td><td>5</td></tr> | ||
<tr bgcolor="#f5f5f5"><td>B3</td><td>sep. 16th</td><td> | <tr bgcolor="#f5f5f5"><td>B3</td><td>sep. 16th</td><td>BM2 1:5 with 10 µM spermine</td><td>sep. 21th</td><td>5</td></tr> | ||
<tr bgcolor="#f5f5f5"><td>B4</td><td>sep. 16th</td><td> | <tr bgcolor="#f5f5f5"><td>B4</td><td>sep. 16th</td><td>BM2 1:5 with 30 µM spermine</td><td>sep. 21th</td><td>5</td></tr> | ||
<tr bgcolor="#f5f5f5"><td>B5</td><td>sep. 16th</td><td> | <tr bgcolor="#f5f5f5"><td>B5</td><td>sep. 16th</td><td>BM2 1:5 with 100 µM spermine</td><td>sep. 21th</td><td>5</td></tr> | ||
<tr bgcolor="#f5f5f5"><td>C2</td><td>sep. 16th</td><td> | <tr bgcolor="#f5f5f5"><td>C2</td><td>sep. 16th</td><td>BM2 1:5 with 25 µM ethidium bromide</td><td>sep. 21th</td><td>5</td></tr> | ||
<tr bgcolor="#f5f5f5"><td>C3</td><td>sep. 16th</td><td> | <tr bgcolor="#f5f5f5"><td>C3</td><td>sep. 16th</td><td>BM2 1:5 with 75 µM ethidium bromide</td><td>sep. 21th</td><td>5</td></tr> | ||
<tr bgcolor="#f5f5f5"><td>C4</td><td>sep. 16th</td><td> | <tr bgcolor="#f5f5f5"><td>C4</td><td>sep. 16th</td><td>BM2 1:5 with 250 µM ethidium bromide</td><td>sep. 21th</td><td>5</td></tr> | ||
<tr><td></td></tr> | <tr><td></td></tr> | ||
<tr bgcolor="#f5f5f5"><td>C5</td><td>sep. 28th</td><td> | <tr bgcolor="#f5f5f5"><td>C5</td><td>sep. 28th</td><td>BM2 1:5 with control without DNA-binders</td><td>4</td><td>5</td></tr> | ||
<tr bgcolor="#f5f5f5"><td>D1</td><td>sep. 28th</td><td> | <tr bgcolor="#f5f5f5"><td>D1</td><td>sep. 28th</td><td>BM2 1:5 with 6 µM ethidium bromide</td><td>4</td><td>5</td></tr> | ||
<tr bgcolor="#f5f5f5"><td>D2</td><td>sep. 28th</td><td> | <tr bgcolor="#f5f5f5"><td>D2</td><td>sep. 28th</td><td>BM2 1:5 with 12µM ethidium bromide</td><td>4</td><td>5</td></tr> | ||
<tr bgcolor="#f5f5f5"><td>D3</td><td>sep. 28th</td><td> | <tr bgcolor="#f5f5f5"><td>D3</td><td>sep. 28th</td><td>BM2 1:5 with 2.4 µM spermine</td><td>4</td><td>5</td></tr> | ||
<tr bgcolor="#f5f5f5"><td>D4</td><td>sep. 28th</td><td> | <tr bgcolor="#f5f5f5"><td>D4</td><td>sep. 28th</td><td>BM2 1:5 with 4.8 µM spermine</td><td>4</td><td>5</td></tr> | ||
<tr bgcolor="#f5f5f5"><td>E1</td><td>sep. 28th</td><td> | <tr bgcolor="#f5f5f5"><td>E1</td><td>sep. 28th</td><td>BM2 1:5 with 0.9 nM DAPI</td><td>4</td><td>5</td></tr> | ||
<tr bgcolor="#f5f5f5"><td>E2</td><td>sep. 28th</td><td> | <tr bgcolor="#f5f5f5"><td>E2</td><td>sep. 28th</td><td>BM2 1:5 with 1.8 nM DAPI</td><td>4</td><td>5</td></tr> | ||
Revision as of 13:05, 2 November 2011
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TEM
http://openwetware.org/index.php?title=Biomod/2011/TUM/TNT/LabbookA/list_TEM_pictures&action=edit
All dilutions of the structures were made with FOB20. For the calculation of the intercalator concentration read more in the corresponding labbook entry.
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<tr bgcolor="#f5f5f5"><td><b>grid number </b></td><td><b>prepared </b></td><td><b>annotations </b></td><td><b>observed </b></td><td><b>annotation</b></td></tr>
<tr><td></td></tr> <tr bgcolor="#f5f5f5"><td>B2</td><td>sep. 16th</td><td>BM2 1:5 control without DNA-binders</td><td>sep. 21th</td><td>5</td></tr> <tr bgcolor="#f5f5f5"><td>B3</td><td>sep. 16th</td><td>BM2 1:5 with 10 µM spermine</td><td>sep. 21th</td><td>5</td></tr> <tr bgcolor="#f5f5f5"><td>B4</td><td>sep. 16th</td><td>BM2 1:5 with 30 µM spermine</td><td>sep. 21th</td><td>5</td></tr> <tr bgcolor="#f5f5f5"><td>B5</td><td>sep. 16th</td><td>BM2 1:5 with 100 µM spermine</td><td>sep. 21th</td><td>5</td></tr> <tr bgcolor="#f5f5f5"><td>C2</td><td>sep. 16th</td><td>BM2 1:5 with 25 µM ethidium bromide</td><td>sep. 21th</td><td>5</td></tr> <tr bgcolor="#f5f5f5"><td>C3</td><td>sep. 16th</td><td>BM2 1:5 with 75 µM ethidium bromide</td><td>sep. 21th</td><td>5</td></tr> <tr bgcolor="#f5f5f5"><td>C4</td><td>sep. 16th</td><td>BM2 1:5 with 250 µM ethidium bromide</td><td>sep. 21th</td><td>5</td></tr>
<tr><td></td></tr>
<tr bgcolor="#f5f5f5"><td>C5</td><td>sep. 28th</td><td>BM2 1:5 with control without DNA-binders</td><td>4</td><td>5</td></tr>
<tr bgcolor="#f5f5f5"><td>D1</td><td>sep. 28th</td><td>BM2 1:5 with 6 µM ethidium bromide</td><td>4</td><td>5</td></tr>
<tr bgcolor="#f5f5f5"><td>D2</td><td>sep. 28th</td><td>BM2 1:5 with 12µM ethidium bromide</td><td>4</td><td>5</td></tr>
<tr bgcolor="#f5f5f5"><td>D3</td><td>sep. 28th</td><td>BM2 1:5 with 2.4 µM spermine</td><td>4</td><td>5</td></tr>
<tr bgcolor="#f5f5f5"><td>D4</td><td>sep. 28th</td><td>BM2 1:5 with 4.8 µM spermine</td><td>4</td><td>5</td></tr>
<tr bgcolor="#f5f5f5"><td>E1</td><td>sep. 28th</td><td>BM2 1:5 with 0.9 nM DAPI</td><td>4</td><td>5</td></tr>
<tr bgcolor="#f5f5f5"><td>E2</td><td>sep. 28th</td><td>BM2 1:5 with 1.8 nM DAPI</td><td>4</td><td>5</td></tr>
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<tr bgcolor="#f5f5f5"><td valign="top"><b>grid number </b></td><td valign="top"><b>prepared </b></td><td valign="top"><b>annotations </b></td><td valign="top"><b>observed </b></td><td valign="top"><b>annotation</b></td></tr>
<tr><td valign="top"></td></tr>
<tr bgcolor="#f5f5f5"><td valign="top">A1</td><td valign="top">oct. 5th</td><td valign="top">BM2 1:20 with 1.34 µM spermine (corresponding to one molecule every 7bp)</td><td valign="top">oct. 7th</td><td valign="top">good stain, slightly to low dilution, 62 images</td></tr> <tr bgcolor="#f5f5f5"><td valign="top">A2</td><td valign="top">oct. 5th</td><td valign="top">BM2 1:20 with 1.34 µM spermine (corresponding to one molecule every 7bp)</td><td valign="top">oct. 7th</td><td valign="top">good stain, slightly to low dilution, 63 images</td></tr> <tr bgcolor="#f5f5f5"><td valign="top">A3</td><td valign="top">oct. 5th</td><td valign="top">BM2 1:20 with 1.34 µM spermine (corresponding to one molecule every 7bp)</td><td valign="top">oct. 7th</td><td valign="top">good stain, slightly to low dilution, 60 images</td></tr> <tr bgcolor="#f5f5f5"><td valign="top">A4</td><td valign="top">oct. 5th</td><td valign="top">BM2 1:20 with 0.42 µM spermine (corresponding to one molecule every 21bp)</td><td valign="top">oct. 7th</td><td valign="top">good stain, slightly to low dilution, 64 images</td></tr> <tr bgcolor="#f5f5f5"><td valign="top">A5</td><td valign="top">oct. 5th</td><td valign="top">BM2 1:20 with 0.42 µM spermine (corresponding to one molecule every 21bp)</td><td valign="top">oct. 7th</td><td valign="top">good stain, slightly to low dilution, 63 images</td></tr> <tr bgcolor="#f5f5f5"><td valign="top">A6</td><td valign="top">oct. 20th</td><td valign="top">BM2 1:25 with 432 nM DAPI (corresponding to one molecule every 7bp)</td><td valign="top">oct. 28st</td><td valign="top">no origamis on the grid, no images</td></tr> <tr bgcolor="#f5f5f5"><td valign="top">A7</td><td valign="top">oct. 20th</td><td valign="top">BM2 1:25 with 144 nM DAPI (corresponding to one molecule every 21bp)</td><td valign="top">oct. 27th</td><td valign="top">no origamis on the grid, no images</td></tr> <tr bgcolor="#f5f5f5"><td valign="top">A8</td><td valign="top">oct. 20th</td><td valign="top">BM2 1:25 with 144 nM DAPI (corresponding to one molecule every 21bp)</td><td valign="top">oct. 28th<td valign="top">no origamis on the grid, no images</td></tr> <tr bgcolor="#f5f5f5"><td valign="top">A9</td><td valign="top">oct. 20th</td><td valign="top">BM2 1:25 without any DNA binders</td><td valign="top">oct. 21th</td><td valign="top">good stain and dilution, 40 images</td></tr> <tr bgcolor="#f5f5f5"><td valign="top">A10</td><td valign="top">oct. 20th</td><td valign="top">BM2 1:25 without any DNA binders</td><td valign="top">oct. 21th</td><td valign="top">good stain and dilution, 60 images</td></tr>
<tr><td valign="top"></td></tr> <tr bgcolor="#f5f5f5"><td valign="top">B1</td><td valign="top">oct. 5th</td><td valign="top">BM2 1:20 with 0.42 µM spermine (corresponding to one molecule every 21bp)</td><td valign="top">oct. 12th</td><td valign="top">grid was fine, 60 images</td></tr> <tr bgcolor="#f5f5f5"><td valign="top">B2</td><td valign="top">oct. 10th</td><td valign="top">BM2 1:40 with 2.27 µM ethidium bromide (corresponding to one molecule every 7bp)</td><td valign="top">oct. 12th</td><td valign="top">too high dilution, 25 images</td></tr> <tr bgcolor="#f5f5f5"><td valign="top">B3</td><td valign="top">oct. 10th</td><td valign="top">BM2 1:40 with 2.27 µM ethidium bromide (corresponding to one molecule every 7bp)</td><td valign="top">oct. 13th</td><td valign="top">good stain and structure concentration, 36 images</td></tr> <tr bgcolor="#f5f5f5"><td valign="top">B4</td><td valign="top">oct. 10th</td><td valign="top">BM2 1:40 with 2.27 µM ethidium bromide (corresponding to one molecule every 7bp)</td><td valign="top">oct. 14th</td><td valign="top">good stain but slightly to low structure concentration, 35 images</td></tr> <tr bgcolor="#f5f5f5"><td valign="top">B5</td><td valign="top">oct. 10th</td><td valign="top">BM2 1:40 with 0.69 µM ethidium bromide (corresponding to one molecule every 21bp)</td><td valign="top">4</td><td valign="top">5</td></tr> <tr bgcolor="#f5f5f5"><td valign="top">B6</td><td valign="top">oct. 20th</td><td valign="top">BM2 without any DNA binders, 1:25</td><td valign="top">oct. 21th</td><td valign="top">good stain and dilution, 66 images</td></tr> <tr bgcolor="#f5f5f5"><td valign="top">B7</td><td valign="top">oct. 20th</td><td valign="top">BM2 without any DNA binders, 1:25</td><td valign="top">oct. 21th</td><td valign="top">good stain and dilution, 80 images</td></tr> <tr bgcolor="#f5f5f5"><td valign="top">B8</td><td valign="top">oct. 20th</td><td valign="top">BM24 15_65 1:25 without any DNA binders</td><td valign="top">oct. 20th</td><td valign="top">bad stain and to high dilution</td></tr> <tr bgcolor="#f5f5f5"><td valign="top">B9</td><td valign="top">oct. 20th</td><td valign="top">BM24 2DH3ML 1:25 without any DNA binders </td><td valign="top">oct. 20th</td><td valign="top">bad stain, no structures on the grid, 3 images</td></tr> <tr bgcolor="#f5f5f5"><td valign="top">B10</td><td valign="top">oct. 21th</td><td valign="top">BM24 15_65 1:20 without any DNA binders</td><td valign="top">oct. 21th</td><td valign="top">good stain but slightly to high dilution, 61 images</td></tr>
<tr><td valign="top"></td></tr> <tr bgcolor="#f5f5f5"><td valign="top">C1</td><td valign="top">oct. 10th</td><td valign="top">BM2 1:40 with 0.69 µM ethidium bromide (corresponding to one molecule every 21bp)</td><td valign="top">oct. 14th</td><td valign="top">good stain but dilution slightly to low, 32 images</td></tr> <tr bgcolor="#f5f5f5"><td valign="top">C2</td><td valign="top">oct. 10th</td><td valign="top">BM2 1:40 with 0.69 µM ethidium bromide (corresponding to one molecule every 21bp)</td><td valign="top">oct. 14th</td><td valign="top">good stain but dilution slightly to low, 31 images</td></tr> <tr bgcolor="#f5f5f5"><td valign="top">C3</td><td valign="top">oct. 10th</td><td valign="top">BM2 1:40 without any DNA binders</td><td valign="top">4</td><td valign="top">5</td></tr> <tr bgcolor="#f5f5f5"><td valign="top">C4</td><td valign="top">oct. 10th</td><td valign="top">BM2 1:40 without any DNA binders</td><td valign="top">4</td><td valign="top">5</td></tr> <tr bgcolor="#f5f5f5"><td valign="top">C5</td><td valign="top">oct. 10th</td><td valign="top">BM2 1:40 without any DNA binders</td><td valign="top">4</td><td valign="top">5</td></tr> <tr bgcolor="#f5f5f5"><td valign="top">C6</td><td valign="top">oct. 21th</td><td valign="top">BM24 15_65 dilution 1:20 without any DNA binders</td><td valign="top">oct. 21th</td><td valign="top">stain was bad and to high dilution, 17 images</td></tr> <tr bgcolor="#f5f5f5"><td valign="top">C7</td><td valign="top">oct. 21th</td><td valign="top">BM24 2DH3ML dilution 1:20 without any DNA binders </td><td valign="top">oct. 21th</td><td valign="top">stain was good but dilution slightly to high, 70 images</td></tr> <tr bgcolor="#f5f5f5"><td valign="top">C8</td><td valign="top">oct. 21th</td><td valign="top">BM24 2DH3ML 1:20 without any DNA binders</td><td valign="top">oct. 21th</td><td valign="top">stain was good but dilution slightly to high</td></tr> <tr bgcolor="#f5f5f5"><td valign="top">C9</td><td valign="top">oct. 27th</td><td valign="top">BM2 1:25 without any DNA binders</td><td valign="top">oct. 27</td><td valign="top">good stain but to high dilution, 60 images</td></tr> <tr bgcolor="#f5f5f5"><td valign="top">C10</td><td valign="top">oct. 27th</td><td valign="top">BM2 1:25 without any DNA binders</td><td valign="top">oct. 27th</td><td valign="top">fine grid, 60 images</td></tr>
<tr><td valign="top"></td></tr> <tr bgcolor="#f5f5f5"><td valign="top">D1</td><td valign="top">oct. 12th</td><td valign="top">BM2 1:25 with 2.4 µM ethidium bromide (corresponding to one molecule every 7bp)</td><td valign="top">oct. 12th</td><td valign="top">good stain and dilution, 74 images</td></tr> <tr bgcolor="#f5f5f5"><td valign="top">D2</td><td valign="top">oct. 12th</td><td valign="top">BM2 1:25 with 0.74 µM ethidium bromide (corresponding to one molecule every 21bp)</td><td valign="top">oct. 12th</td><td valign="top">good stain and dilution, 89 images, chosen for class average</td></tr> <tr bgcolor="#f5f5f5"><td valign="top">D3</td><td valign="top">oct. 12th</td><td valign="top">BM2 1:25without any DNA binders </td><td valign="top">oct. 12th</td><td valign="top">good stain and dilution, 50 images</td></tr> <tr bgcolor="#f5f5f5"><td valign="top">D4</td><td valign="top">oct. 14th</td><td valign="top">BM2 1:25 with 432 µM DAPI (corresponding to one molecule every 7bp)</td><td valign="top">oct. 20th</td><td valign="top">good stain and structur concentration but cluster, 62 images</td></tr> <tr bgcolor="#f5f5f5"><td valign="top">D5</td><td valign="top">oct. 14th</td><td valign="top">BM2 1:25 with 432 nM DAPI (corresponding to one molecule every 7bp)</td><td valign="top">oct. 20th</td><td valign="top">stain and dilution was good, cluster, 61 images</td></tr> <tr bgcolor="#f5f5f5"><td valign="top">D6</td><td valign="top">oct. 27th</td><td valign="top">BM24 (15_65), 1:20 diluted with FOB20</td><td valign="top">oct. 27th</td><td valign="top">good stain but slightly to high dilution, 22 images</td></tr> <tr bgcolor="#f5f5f5"><td valign="top">D7</td><td valign="top">oct. 27th</td><td valign="top">lost the grid before sample preparation</td><td valign="top">4</td><td valign="top">5</td></tr> <tr bgcolor="#f5f5f5"><td valign="top">D8</td><td valign="top">oct. 27th</td><td valign="top">BM24 (15_65) 1:10 without any DNA binders</td><td valign="top">oct. 27th</td><td valign="top">low sample concentration, 24 images</td></tr> <tr bgcolor="#f5f5f5"><td valign="top">D9</td><td valign="top">oct. 27th</td><td valign="top">BM24 (15_65) 1:10 without any DNA binders</td><td valign="top">4</td><td valign="top">,stain was good but dilution slightly to low</td></tr> <tr bgcolor="#f5f5f5"><td valign="top">D10</td><td valign="top">oct. 28st</td><td valign="top">BM24 (15_65) 1:5 without any DNA binders</td><td valign="top">oct. 28st</td><td valign="top"> better sample concentration than D6 and D8, 60 images</td></tr>
<tr><td valign="top"></td></tr> <tr bgcolor="#f5f5f5"><td valign="top">E1</td><td valign="top">oct. 20th</td><td valign="top">BM2 1:25 with 2.4 µM EtBr (corresponding to one molecule every 7bp)</td><td valign="top">oct. 26th</td><td valign="top">good dilution, bad staining, 49 images</td></tr> <tr bgcolor="#f5f5f5"><td valign="top">E2</td><td valign="top">oct. 20th</td><td valign="top">BM2 1:25 with 2.4 µM EtBr (corresponding to one molecule every 7bp)</td><td valign="top">oct. 26th</td><td valign="top">good dilution but strong background, 50 images</td></tr> <tr bgcolor="#f5f5f5"><td valign="top">E3</td><td valign="top">oct. 20th</td><td valign="top">BM2 1:25 with 0.74 µM EtBr (corresponding to one molecule every 21bp)<td valign="top">oct. 26th</td><td valign="top">fine grid, 60 images</td></tr> <tr bgcolor="#f5f5f5"><td valign="top">E4</td><td valign="top">oct. 20th</td><td valign="top">BM2 1:25 with 0.74 µM EtBr (corresponding to one molecule every 21bp)<td valign="top">oct. 26th</td><td valign="top">fine grid, 50 images</td></tr> <tr bgcolor="#f5f5f5"><td valign="top">E5</td><td valign="top">oct. 20th</td><td valign="top">BM2 1:25 with 432 nM DAPI (corresponding to one molecule every 7bp)</td><td valign="top">oct. 26th</td><td valign="top">good dilution but weak stain, 60 images</td></tr> <tr bgcolor="#f5f5f5"><td valign="top">E6</td><td valign="top">oct. 28st</td><td valign="top">BM24 (15_65), 1:5</td><td valign="top">oct. 28st</td><td valign="top">fine grid, 413 images</td></tr> <tr bgcolor="#f5f5f5"><td valign="top">E7</td><td valign="top">oct. 31th</td><td valign="top">BM2 1:20 with 6.7µM spermine (corresponding to one molecule every 2bp)</td><td valign="top">oct. 31th</td><td valign="top">grid was fine, 112 images</td></tr> <tr bgcolor="#f5f5f5"><td valign="top">E8</td><td valign="top">oct. 31th</td><td valign="top">BM2 1:20 with 6.7µM spermine (corresponding to one molecule every 2bp)</td><td valign="top">oct. 31th</td><td valign="top">excellent grid, 80 images</td></tr> <tr bgcolor="#f5f5f5"><td valign="top">E9</td><td valign="top">nov. 1st</td><td valign="top">BM2 1:20 with 12.1µM spermine (corresponding to one molecule every 1.5bp)</td><td valign="top">nov. 1st</td><td valign="top">good grid, 111 images</td></tr> <tr bgcolor="#f5f5f5"><td valign="top">E10</td><td valign="top">nov. 1st</td><td valign="top">BM2 1:20 with 12.1µM spermine (corresponding to one molecule every 1.5bp)</td><td valign="top">nov. 1st</td><td valign="top">fine grid, 131 images</td></tr>
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