Biomod/2011/TUM/TNT/LabbookA/list TEM pictures

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http://openwetware.org/index.php?title=Biomod/2011/TUM/TNT/LabbookA/list_TEM_pictures&action=edit

summary of all prepared and observed grids

All dilutions were made with FOB20. For the calculation of the intercalator concentration read more in the corresponding labbook entry.


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<tr bgcolor="#f5f5f5"><td><b>grid number&nbsp;&nbsp;&nbsp;</b></td><td><b>prepared&nbsp;&nbsp;&nbsp;</b></td><td><b>annotations&nbsp;&nbsp;&nbsp;</b></td><td><b>observed&nbsp;&nbsp;&nbsp;</b></td><td><b>annotation</b></td></tr>

<tr><td></td></tr> <tr bgcolor="#f5f5f5"><td>A1</td><td>2</td><td>3</td><td>4</td><td>5</td></tr> <tr bgcolor="#f5f5f5"><td>A2</td><td>2</td><td>3</td><td>4</td><td>5</td></tr> <tr bgcolor="#f5f5f5"><td>A3</td><td>2</td><td>3</td><td>4</td><td>5</td></tr> <tr bgcolor="#f5f5f5"><td>A4</td><td>2</td><td>3</td><td>4</td><td>5</td></tr> <tr bgcolor="#f5f5f5"><td>A5</td><td>2</td><td>3</td><td>4</td><td>5</td></tr> <tr bgcolor="#f5f5f5"><td>A6</td><td>2</td><td>3</td><td>4</td><td>5</td></tr> <tr bgcolor="#f5f5f5"><td>A7</td><td>2</td><td>3</td><td>4</td><td>5</td></tr> <tr bgcolor="#f5f5f5"><td>A8</td><td>2</td><td>3</td><td>4</td><td>5</td></tr> <tr bgcolor="#f5f5f5"><td>A9</td><td>2</td><td>3</td><td>4</td><td>5</td></tr> <tr bgcolor="#f5f5f5"><td>A10</td><td>2</td><td>3</td><td>4</td><td>5</td></tr>

<tr><td></td></tr> <tr bgcolor="#f5f5f5"><td>B1</td><td>2</td><td>3</td><td>4</td><td>5</td></tr> <tr bgcolor="#f5f5f5"><td>B2</td><td>2</td><td>3</td><td>4</td><td>5</td></tr> <tr bgcolor="#f5f5f5"><td>B3</td><td>2</td><td>3</td><td>4</td><td>5</td></tr> <tr bgcolor="#f5f5f5"><td>B4</td><td>2</td><td>3</td><td>4</td><td>5</td></tr> <tr bgcolor="#f5f5f5"><td>B5</td><td>2</td><td>3</td><td>4</td><td>5</td></tr> <tr bgcolor="#f5f5f5"><td>B6</td><td>2</td><td>3</td><td>4</td><td>5</td></tr> <tr bgcolor="#f5f5f5"><td>B7</td><td>2</td><td>3</td><td>4</td><td>5</td></tr> <tr bgcolor="#f5f5f5"><td>B8</td><td>2</td><td>3</td><td>4</td><td>5</td></tr> <tr bgcolor="#f5f5f5"><td>B9</td><td>2</td><td>3</td><td>4</td><td>5</td></tr> <tr bgcolor="#f5f5f5"><td>B10</td><td>2</td><td>3</td><td>4</td><td>5</td></tr>

<tr><td></td></tr> <tr bgcolor="#f5f5f5"><td>C1</td><td>2</td><td>3</td><td>4</td><td>5</td></tr> <tr bgcolor="#f5f5f5"><td>C2</td><td>2</td><td>3</td><td>4</td><td>5</td></tr> <tr bgcolor="#f5f5f5"><td>C3</td><td>2</td><td>3</td><td>4</td><td>5</td></tr> <tr bgcolor="#f5f5f5"><td>C4</td><td>2</td><td>3</td><td>4</td><td>5</td></tr> <tr bgcolor="#f5f5f5"><td>C5</td><td>2</td><td>3</td><td>4</td><td>5</td></tr> <tr bgcolor="#f5f5f5"><td>C6</td><td>2</td><td>3</td><td>4</td><td>5</td></tr> <tr bgcolor="#f5f5f5"><td>C7</td><td>2</td><td>3</td><td>4</td><td>5</td></tr> <tr bgcolor="#f5f5f5"><td>C8</td><td>2</td><td>3</td><td>4</td><td>5</td></tr> <tr bgcolor="#f5f5f5"><td>C9</td><td>2</td><td>3</td><td>4</td><td>5</td></tr> <tr bgcolor="#f5f5f5"><td>C10</td><td>2</td><td>3</td><td>4</td><td>5</td></tr>

<tr><td></td></tr> <tr bgcolor="#f5f5f5"><td>D1</td><td>2</td><td>3</td><td>4</td><td>5</td></tr> <tr bgcolor="#f5f5f5"><td>D2</td><td>2</td><td>3</td><td>4</td><td>5</td></tr> <tr bgcolor="#f5f5f5"><td>D3</td><td>2</td><td>3</td><td>4</td><td>5</td></tr> <tr bgcolor="#f5f5f5"><td>D4</td><td>2</td><td>3</td><td>4</td><td>5</td></tr> <tr bgcolor="#f5f5f5"><td>D5</td><td>2</td><td>3</td><td>4</td><td>5</td></tr> <tr bgcolor="#f5f5f5"><td>D6</td><td>2</td><td>3</td><td>4</td><td>5</td></tr> <tr bgcolor="#f5f5f5"><td>D7</td><td>2</td><td>3</td><td>4</td><td>5</td></tr> <tr bgcolor="#f5f5f5"><td>D8</td><td>2</td><td>3</td><td>4</td><td>5</td></tr> <tr bgcolor="#f5f5f5"><td>D9</td><td>2</td><td>3</td><td>4</td><td>5</td></tr> <tr bgcolor="#f5f5f5"><td>D10</td><td>2</td><td>3</td><td>4</td><td>5</td></tr>

<tr><td></td></tr> <tr bgcolor="#f5f5f5"><td>E1</td><td>2</td><td>3</td><td>4</td><td>5</td></tr> <tr bgcolor="#f5f5f5"><td>E2</td><td>2</td><td>3</td><td>4</td><td>5</td></tr> <tr bgcolor="#f5f5f5"><td>E3</td><td>2</td><td>3</td><td>4</td><td>5</td></tr> <tr bgcolor="#f5f5f5"><td>E4</td><td>2</td><td>3</td><td>4</td><td>5</td></tr> <tr bgcolor="#f5f5f5"><td>E5</td><td>2</td><td>3</td><td>4</td><td>5</td></tr> <tr bgcolor="#f5f5f5"><td>E6</td><td>2</td><td>3</td><td>4</td><td>5</td></tr> <tr bgcolor="#f5f5f5"><td>E7</td><td>2</td><td>3</td><td>4</td><td>5</td></tr> <tr bgcolor="#f5f5f5"><td>E8</td><td>2</td><td>3</td><td>4</td><td>5</td></tr> <tr bgcolor="#f5f5f5"><td>E9</td><td>2</td><td>3</td><td>4</td><td>5</td></tr> <tr bgcolor="#f5f5f5"><td>E10</td><td>2</td><td>3</td><td>4</td><td>5</td></tr>

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BIOMOD2 grid box

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<tr bgcolor="#f5f5f5"><td valign="top"><b>grid number&nbsp;&nbsp;&nbsp;</b></td><td valign="top"><b>observed&nbsp;&nbsp;&nbsp;</b></td><td valign="top"><b>annotations&nbsp;&nbsp;&nbsp;</b></td><td valign="top"><b>observed&nbsp;&nbsp;&nbsp;</b></td><td valign="top"><b>annotation</b></td></tr>

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<tr bgcolor="#f5f5f5"><td valign="top">A1</td><td valign="top">oct. 5th</td><td valign="top">BM2 1:20 with 1.34 µM spermine (corresponding to one molecule every 7bp)</td><td valign="top">oct. 7th</td><td valign="top">good stain, slightly to high structure concentration, 60 images</td></tr> <tr bgcolor="#f5f5f5"><td valign="top">A2</td><td valign="top">oct. 5th</td><td valign="top">BM2 1:20 with 1.34 µM spermine (corresponding to one molecule every 7bp)</td><td valign="top">oct. 7th</td><td valign="top">good stain, slightly to high structure concentration, 60 images</td></tr> <tr bgcolor="#f5f5f5"><td valign="top">A3</td><td valign="top">oct. 5th</td><td valign="top">BM2 1:20 with 1.34 µM spermine (corresponding to one molecule every 7bp)</td><td valign="top">oct. 7th</td><td valign="top">good stain, slightly to high structure concentration, 60 images</td></tr> <tr bgcolor="#f5f5f5"><td valign="top">A4</td><td valign="top">oct. 5th</td><td valign="top">BM2 1:20 with 0.42 µM spermine (corresponding to one molecule every 21bp)</td><td valign="top">oct. 7th</td><td valign="top">good stain, slightly to high structure concentration, 60 images</td></tr> <tr bgcolor="#f5f5f5"><td valign="top">A5</td><td valign="top">oct. 5th</td><td valign="top">BM2 1:20 with 0.42 µM spermine (corresponding to one molecule every 21bp)</td><td valign="top">oct. 7th</td><td valign="top">good stain, slightly to high structure concentration, 60 images</td></tr> <tr bgcolor="#f5f5f5"><td valign="top">A6</td><td valign="top">oct. 20th</td><td valign="top">BM2 with 432 nM DAPI (corresponding to one molecule every 7bp), 1:25</td><td valign="top">oct. 28st</td><td valign="top">no origamis on the grid</td></tr> <tr bgcolor="#f5f5f5"><td valign="top">A7</td><td valign="top">oct. 20th</td><td valign="top">BM2 with 144 nM DAPI (corresponding to one molecule every 21bp), 1:25</td><td valign="top">oct. 27th</td><td valign="top">no origamis on the grid</td></tr> <tr bgcolor="#f5f5f5"><td valign="top">A8</td><td valign="top">oct. 20th</td><td valign="top">BM2 with 144 nM DAPI (corresponding to one molecule every 21bp), 1:25 </td><td valign="top">oct. 28th<td valign="top">no origamis on the grid</td></tr> <tr bgcolor="#f5f5f5"><td valign="top">A9</td><td valign="top">oct. 20th</td><td valign="top">BM2 without any DNA binders, 1:25</td><td valign="top">oct. 21th</td><td valign="top">stain was good</td></tr> <tr bgcolor="#f5f5f5"><td valign="top">A10</td><td valign="top">oct. 20th</td><td valign="top">BM2 without any DNA binders, 1:25</td><td valign="top">oct. 21th</td><td valign="top">stain was good</td></tr>

<tr><td valign="top"></td></tr> <tr bgcolor="#f5f5f5"><td valign="top">B1</td><td valign="top">oct. 5th</td><td valign="top">BM2 1:20 with 0.42 µM spermine (corresponding to one molecule every 21bp)</td><td valign="top">oct. 12th</td><td valign="top">grid was fine</td></tr> <tr bgcolor="#f5f5f5"><td valign="top">B2</td><td valign="top">oct. 10th</td><td valign="top">BM2 1:40 with 2.27 µM ethidium bromide (corresponding to one molecule every 7bp)</td><td valign="top">oct. 12th</td><td valign="top">too high dilution</td></tr> <tr bgcolor="#f5f5f5"><td valign="top">B3</td><td valign="top">oct. 10th</td><td valign="top">BM2 1:40 with 2.27 µM ethidium bromide (corresponding to one molecule every 7bp)</td><td valign="top">4</td><td valign="top">5</td></tr> <tr bgcolor="#f5f5f5"><td valign="top">B4</td><td valign="top">oct. 10th</td><td valign="top">BM2 1:40 with 2.27 µM ethidium bromide (corresponding to one molecule every 7bp)</td><td valign="top">4</td><td valign="top">5</td></tr> <tr bgcolor="#f5f5f5"><td valign="top">B5</td><td valign="top">oct. 10th</td><td valign="top">BM2 1:40 with 0.69 µM ethidium bromide (corresponding to one molecule every 21bp)</td><td valign="top">4</td><td valign="top">5</td></tr> <tr bgcolor="#f5f5f5"><td valign="top">B6</td><td valign="top">oct. 20th</td><td valign="top">BM2 without any DNA binders, 1:25</td><td valign="top">oct. 21th</td><td valign="top">stain was good</td></tr> <tr bgcolor="#f5f5f5"><td valign="top">B7</td><td valign="top">oct. 20th</td><td valign="top">BM2 without any DNA binders, 1:25</td><td valign="top">oct. 21th</td><td valign="top">stain was good</td></tr> <tr bgcolor="#f5f5f5"><td valign="top">B8</td><td valign="top">oct. 20th</td><td valign="top">BM24 15_65 1:25 without any DNA binders</td><td valign="top">oct. 20th</td><td valign="top">bad stain and to high dilution</td></tr> <tr bgcolor="#f5f5f5"><td valign="top">B9</td><td valign="top">oct. 20th</td><td valign="top">BM24 2DH3ML 1:25 without any DNA binders </td><td valign="top">oct. 20th</td><td valign="top">bad stain, no structures on the grid</td></tr> <tr bgcolor="#f5f5f5"><td valign="top">B10</td><td valign="top">oct. 21th</td><td valign="top">BM24 15_65 1:20 without any DNA binders</td><td valign="top">oct. 21th</td><td valign="top">stain and dilution was good</td></tr>

<tr><td valign="top"></td></tr> <tr bgcolor="#f5f5f5"><td valign="top">C1</td><td valign="top">oct. 10th</td><td valign="top">BM2 1:40 with 0.69 µM ethidium bromide (corresponding to one molecule every 21bp)</td><td valign="top">4</td><td valign="top">5</td></tr> <tr bgcolor="#f5f5f5"><td valign="top">C2</td><td valign="top">oct. 10th</td><td valign="top">BM2 1:40 with 0.69 µM ethidium bromide (corresponding to one molecule every 21bp)</td><td valign="top">4</td><td valign="top">5</td></tr> <tr bgcolor="#f5f5f5"><td valign="top">C3</td><td valign="top">oct. 10th</td><td valign="top">BM2 1:40 without any DNA binders</td><td valign="top">4</td><td valign="top">5</td></tr> <tr bgcolor="#f5f5f5"><td valign="top">C4</td><td valign="top">oct. 10th</td><td valign="top">BM2 1:40 without any DNA binders</td><td valign="top">4</td><td valign="top">5</td></tr> <tr bgcolor="#f5f5f5"><td valign="top">C5</td><td valign="top">oct. 10th</td><td valign="top">BM2 1:40 without any DNA binders</td><td valign="top">4</td><td valign="top">5</td></tr> <tr bgcolor="#f5f5f5"><td valign="top">C6</td><td valign="top">oct. 21th</td><td valign="top">BM24 15_65 dilution 1:20 without any DNA binders</td><td valign="top">oct. 21th</td><td valign="top">stain was bad</td></tr> <tr bgcolor="#f5f5f5"><td valign="top">C7</td><td valign="top">oct. 21th</td><td valign="top">BM24 2DH3ML dilution 1:20 without any DNA binders </td><td valign="top">oct. 21th</td><td valign="top">stain was good but dilution slightly to high</td></tr> <tr bgcolor="#f5f5f5"><td valign="top">C8</td><td valign="top">oct. 21th</td><td valign="top">BM24 2DH3ML 1:20 without any DNA binders</td><td valign="top">oct. 21th</td><td valign="top">stain was good but dilution slightly to high</td></tr> <tr bgcolor="#f5f5f5"><td valign="top">C9</td><td valign="top">oct. 27th</td><td valign="top">BM2 1:25 without any DNA binders</td><td valign="top">4</td><td valign="top">5</td></tr> <tr bgcolor="#f5f5f5"><td valign="top">C10</td><td valign="top">oct. 27th</td><td valign="top">BM2 1:25 without any DNA binders</td><td valign="top">oct. 27th</td><td valign="top">fine grid, 60 images</td></tr>

<tr><td valign="top"></td></tr> <tr bgcolor="#f5f5f5"><td valign="top">D1</td><td valign="top">oct. 12th</td><td valign="top">BM2 1:25 with 2.4 µM ethidium bromide (corresponding to one molecule every 7bp)</td><td valign="top">oct. 12th</td><td valign="top">good stain and dilution</td></tr> <tr bgcolor="#f5f5f5"><td valign="top">D2</td><td valign="top">oct. 12th</td><td valign="top">BM2 1:25 with 0.74 µM ethidium bromide (corresponding to one molecule every 21bp)</td><td valign="top">oct. 12th</td><td valign="top">good stain and dilution</td></tr> <tr bgcolor="#f5f5f5"><td valign="top">D3</td><td valign="top">oct. 12th</td><td valign="top">BM2 1:25without any DNA binders </td><td valign="top">oct. 12th</td><td valign="top">good stain and dilution</td></tr> <tr bgcolor="#f5f5f5"><td valign="top">D4</td><td valign="top">oct. 14th</td><td valign="top">BM2 1:25 with 432 µM DAPI (corresponding to one molecule every 7bp)</td><td valign="top">oct. 20th</td><td valign="top">stain and dilution was good, cluster</td></tr> <tr bgcolor="#f5f5f5"><td valign="top">D5</td><td valign="top">oct. 14th</td><td valign="top">BM2 1:25 with 432 nM DAPI (corresponding to one molecule every 7bp)</td><td valign="top">oct. 20th</td><td valign="top">stain and dilution was good, cluster</td></tr> <tr bgcolor="#f5f5f5"><td valign="top">D6</td><td valign="top">oct. 27th</td><td valign="top">BM24 (15_65), 1:20 diluted with FOB20</td><td valign="top">4</td><td valign="top">5</td></tr> <tr bgcolor="#f5f5f5"><td valign="top">D7</td><td valign="top">oct. 27th</td><td valign="top">lost the grid before sample preparation</td><td valign="top">4</td><td valign="top">5</td></tr> <tr bgcolor="#f5f5f5"><td valign="top">D8</td><td valign="top">oct. 27th</td><td valign="top">BM24 (15_65) 1:10 without any DNA binders</td><td valign="top">oct. 27th</td><td valign="top">low sample concentration, 24 images</td></tr> <tr bgcolor="#f5f5f5"><td valign="top">D9</td><td valign="top">oct. 27th</td><td valign="top">BM24 (15_65) 1:10 without any DNA binders</td><td valign="top">4</td><td valign="top">5</td></tr> <tr bgcolor="#f5f5f5"><td valign="top">D10</td><td valign="top">oct. 28st</td><td valign="top">BM24 (15_65) 1:5 without any DNA binders</td><td valign="top">oct. 28st</td><td valign="top"> better sample concentration than 2D6 and 2D8, 40 images</td></tr>

<tr><td valign="top"></td></tr> <tr bgcolor="#f5f5f5"><td valign="top">E1</td><td valign="top">oct. 20th</td><td valign="top">BM2 1:25 with 2.4 µM EtBr (corresponding to one molecule every 7bp)</td><td valign="top">oct. 26th</td><td valign="top">good dilution, bad staining</td></tr> <tr bgcolor="#f5f5f5"><td valign="top">E2</td><td valign="top">oct. 20th</td><td valign="top">BM2 1:25 with 2.4 µM EtBr (corresponding to one molecule every 7bp)</td><td valign="top">oct. 26th</td><td valign="top">strong background</td></tr> <tr bgcolor="#f5f5f5"><td valign="top">E3</td><td valign="top">oct. 20th</td><td valign="top">BM2 1:25 with 0.74 µM EtBr (corresponding to one molecule every 21bp)<td valign="top">oct. 26th</td><td valign="top">fine grid</td></tr> <tr bgcolor="#f5f5f5"><td valign="top">E4</td><td valign="top">oct. 20th</td><td valign="top">BM2 1:25 with 0.74 µM EtBr (corresponding to one molecule every 21bp)<td valign="top">oct. 26th</td><td valign="top">fine grid</td></tr> <tr bgcolor="#f5f5f5"><td valign="top">E5</td><td valign="top">oct. 20th</td><td valign="top">BM2 1:25 with 432 nM DAPI (corresponding to one molecule every 7bp)</td><td valign="top">oct. 26th</td><td valign="top">weak stain</td></tr> <tr bgcolor="#f5f5f5"><td valign="top">E6</td><td valign="top">oct. 28st</td><td valign="top">BM24 (15_65), 1:5</td><td valign="top">oct. 28st</td><td valign="top">fine grid, 100 images</td></tr> <tr bgcolor="#f5f5f5"><td valign="top">E7</td><td valign="top">2</td><td valign="top">3</td><td valign="top">4</td><td valign="top">5</td></tr> <tr bgcolor="#f5f5f5"><td valign="top">E8</td><td valign="top">2</td><td valign="top">3</td><td valign="top">4</td><td valign="top">5</td></tr> <tr bgcolor="#f5f5f5"><td valign="top">E9</td><td valign="top">2</td><td valign="top">3</td><td valign="top">4</td><td valign="top">5</td></tr> <tr bgcolor="#f5f5f5"><td valign="top">E10</td><td valign="top">2</td><td valign="top">3</td><td valign="top">4</td><td valign="top">5</td></tr>

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