Biomod/2011/TUM/TNT/Project: Difference between revisions

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<b>Anmerkung:</b> inhaltlich noch etwas anzupassen, optimal: so dass Bilder dazupassen -> Bilder aussuchen  <br>
<b>Anmerkung:</b> inhaltlich noch etwas anzupassen, optimal: so dass Bilder dazupassen -> Bilder aussuchen  <br>
Hab den Text mal editiert, lests mal drüber, was ihr vom Schluss haltet. Manu
Hab den Text mal editiert, lests mal drüber, was ihr vom Schluss haltet. Manu<b>
..Sorry Manu, ich hab den Text nochmal umgemodelt, ich denke du hast zu viel weggelassen.. Tom
..Sorry Manu, ich hab den Text nochmal umgemodelt, ich denke du hast zu viel weggelassen.. Tom


Revision as of 12:54, 29 October 2011

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<title> 

       TUM NanU - Home

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<body background-color="#F0F0F0"> <!-- Header--> <div class="container"> 

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   <li><a href="http://openwetware.org/wiki/Biomod/2011/TUM/TNT/Home">Home</a></li>
   <li><a href="http://openwetware.org/wiki/Biomod/2011/TUM/TNT/Project">Project</a>        
     <li><a href="http://openwetware.org/wiki/Biomod/2011/TUM/TNT/Results">Results & Discussion</a>
       
   </li>         
   </li>
   <li><a href="http://openwetware.org/wiki/Biomod/2011/TUM/TNT/Methods">Methods</a>    </li>

<li><a href="http://openwetware.org/wiki/Biomod/2011/TUM/TNT/Team">Team</a></li> 

      <li><a href="http://openwetware.org/wiki/Biomod/2011/TUM/TNT/LabbookA">Labbook</a>


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   <li><a href="http://openwetware.org/wiki/Biomod/2011/TUM/TNT/Extras">Extras</a>
       
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Project overview

Abstract

DNA-Origami device to investigate conformational changes of DNA due to interactions with small molecules

Small DNA-binding molecules including intercalators and groove binders cause changes in the geometry of double-helical DNA which may affect transcriptional regulation and nuclease activity. Elucidating the structural changes imposed on DNA including changes in pitch and length can shed light on functional mechanisms in the case of compounds that show selective toxicity such as antibiotic and antiviral activity. Here we report on our efforts in developing a self-assembled DNA-Origami device that can be used both in a fluorometric assay and for transmission electron microscopy with the aim to to classify whether a DNA-binding molecule is an intercalator or groove binder, and that reports quantitatively on changes in pitch and length caused by DNA-binding molecules. Focussing on changes in pitch for the moment, we have tested our device with different DNA binders, spermine, ethidium bromide and DAPI in different concentrations. In accordance with structure simulations, the local deformations caused by addition of these small molecules led to observable global structural changes.

Anmerkung: inhaltlich noch etwas anzupassen, optimal: so dass Bilder dazupassen -> Bilder aussuchen
Hab den Text mal editiert, lests mal drüber, was ihr vom Schluss haltet. Manu ..Sorry Manu, ich hab den Text nochmal umgemodelt, ich denke du hast zu viel weggelassen.. Tom

Streamline


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