Biomod/2011/Tianjin:Results: Difference between revisions
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=Results= | =Results= | ||
== | ==1== | ||
[[Image:Biomod tianjin results 1.png|center|650px|thumb|Fig 1 ]] | [[Image:Biomod tianjin results 1.png|center|650px|thumb|Fig 1.AFM analysis of MreB-RFP cell-free aggregation with SWNTs, indicating that SWNTs serve as scaffolds in the spiral winding of fusion proteins in vitro. | ||
*a). AFM image of fusion protein MreB-RFP without SWNT scaffold, protein aggregated in disorder and only discrete spherical particles were formed. | |||
*b). AFM image of fusion protein MreB-RFP at the presence of SWNT, protein aggregated | |||
and wound spirally onto the surface of nanotube. | |||
]] | |||
== | ==2== | ||
[[Image:Biomod tianjin results 24.png|center|650px|thumb|Fig 2 ]] | [[Image:Biomod tianjin results 24.png|center|650px|thumb|Fig 2. SEM analysis of MreB-RFP cell-free aggregation with SWNTs. | ||
*a). SEM image of SWNT, the sidewall of nanotube is smooth | |||
without any absorption or aggregation. | |||
*b). SEM image of MreB-RFP without scaffold, protein aggregated | |||
in disorder and only discrete spherical particles were formed. | |||
*c). SEM image of fusion protein at the presence of SWNT, protein | |||
aggregated and wound spirally onto the surface of nanotube. | |||
]] | |||
== | ==3== | ||
[[Image:Biomod tianjin results 3.png|center|650px|thumb|Fig 3 ]] | [[Image:Biomod tianjin results 3.png|center|650px|thumb|Fig 3. TEM analysis of MreB-RFP cell-free aggregation with SWNTs. | ||
*a). TEM image of SWNT, the sidewall of nanotube is smooth | |||
without any absorption or aggregation. | |||
*b). TEM image of MreB-RFP without scaffold, protein aggregated | |||
in disorder and only discrete spherical particles were formed. | |||
*c). TEM image of fusion protein without SWNT scaffold, protein | |||
aggregated and wound spirally onto the surface of SWNT. | |||
]] | |||
== | ==4== | ||
[[Image:Biomod tianjin results 4.png|center|500px|thumb|Fig 4 ]] | [[Image:Biomod tianjin results 4.png|center|500px|thumb|Fig 4. Fluorescence micrograph of fusion protein MreB combined with SWNT. Red fluorescence can be observed, indicating the activity of fusion protein were reserved. ]] | ||
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