Biomod/2011/Tianjin:Results: Difference between revisions
From OpenWetWare
Jump to navigationJump to search
No edit summary |
No edit summary |
||
Line 3: | Line 3: | ||
=Results= | =Results= | ||
=='''CFS with microfluidics'''== | =='''CFS with microfluidics'''== | ||
[[Image:Biomod tianjin results 0.png|center| | [[Image:Biomod tianjin results 0.png|center|600px|thumb|Fig 1. Cell-free protein expression in W/O/W. Fluorescence micrograph of a W/O/W during the expression of (a) RFP as a soluble protein in the protocell cytosol, and (b) MreB-RFP, which has portioned at the hydrophobic interface. Figure (c) shows a confocal micrograph (3um thick plane) of a W/O/W, 14 h after generation. The RFP-tagged MreB polymerized, forming aggregates localized to the oil-water interface. The image was created by forming the W/O/W droplets with 100 nM BODIPY dispersed in the oil phase and MreB-RFP in the segmented phase. Fluorescence was detected in separate channels. ]] | ||
Line 9: | Line 9: | ||
=='''CFS with SWNTs'''== | =='''CFS with SWNTs'''== | ||
==='''AFM'''=== | ==='''AFM'''=== | ||
[[Image:Biomod tianjin results 1.png|center| | [[Image:Biomod tianjin results 1.png|center|600px|thumb|Fig 2.AFM analysis of MreB-RFP cell-free aggregation with SWNTs, indicating that SWNTs serve as scaffolds in the spiral winding of fusion proteins in vitro. | ||
a). AFM image of fusion protein MreB-RFP without SWNT scaffold, protein aggregated in disorder and only discrete spherical particles were formed. | a). AFM image of fusion protein MreB-RFP without SWNT scaffold, protein aggregated in disorder and only discrete spherical particles were formed. | ||
b). AFM image of fusion protein MreB-RFP at the presence of SWNT, protein aggregated | b). AFM image of fusion protein MreB-RFP at the presence of SWNT, protein aggregated |