Biomod/2011/Tianjin:Results: Difference between revisions
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Results from fluorescence micrograph and confocal micrograph supported the compatibility of double emulsions with cell-free expression of soluble and structural membrane-related proteins. | Results from fluorescence micrograph and confocal micrograph supported the compatibility of double emulsions with cell-free expression of soluble and structural membrane-related proteins. | ||
[[Image:Biomod tianjin results 0.png|center|600px|thumb|Fig 2. Cell-free protein expression in W/O/W. Fluorescence micrograph of a W/O/W during the expression of (a) RFP as a soluble protein in the protocell cytosol, and (b) MreB-RFP, which has portioned at the hydrophobic interface. Figure (c) shows a confocal micrograph (3um thick plane) of a W/O/W, 14 h after generation. The RFP-tagged MreB polymerized, forming aggregates localized to the oil-water interface. The image was created by forming the W/O/W droplets with 100 nM BODIPY dispersed in the oil phase and MreB-RFP in the segmented phase. Fluorescence was detected in separate channels. ]] | [[Image:Biomod tianjin results 0.png|center|600px|thumb|Fig 2. Cell-free protein expression in W/O/W. Fluorescence micrograph of a W/O/W during the expression of (a) RFP as a soluble protein in the protocell cytosol, and (b) MreB-RFP, which has portioned at the hydrophobic interface. Figure (c) shows a confocal micrograph (3um thick plane) of a W/O/W, 14 h after generation. The RFP-tagged MreB polymerized, forming aggregates localized to the oil-water interface. The image was created by forming the W/O/W droplets with 100 nM BODIPY dispersed in the oil phase and MreB-RFP in the segmented phase. Fluorescence was detected in separate channels. ]] | ||
[[Image:Daibai fenbu.jpg|center|200px|thumb|Fig 3. | [[Image:Daibai fenbu.jpg|center|200px|thumb|Fig 3.Laser scanning confocal microscope demonstrate the synthesis of the actin-like protein MreB and observed its preference to localize and aggregate at the protocell interface.]] | ||