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There were two subproblems to solve in approaching the templating problem. First, we experimented with three different D-DNA template designs; [[Biomod/2012/Harvard/BioDesign/small_canvas_SST |a small SST canvas]], [[Biomod/2012/Harvard/BioDesign/large_canvas_SST |a large SST canvas]], and [[Biomod/2012/Harvard/BioDesign/DNA_origami |a 3d DNA origami structure]]. Second, we optimized the nucleotide sequence of the [[Biomod/2012/Harvard/BioDesign/L-DNA_layer |L-DNA layer]] for infinite tiling and a low melting temperature.
 
<font size="5">Approaches</font>
 
 
Our project had multiple parallel pathways.  There were two sub-problems to solve in approaching the templating problem.  
 
One was the templating technique, for which we experimented with three different D-DNA designs;
 
[[Biomod/2012/Harvard/BioDesign/small_canvas_SST |Small SST canvas]]
<br>
[[Biomod/2012/Harvard/BioDesign/large_canvas_SST |Large SST canvas]]
<br>
[[Biomod/2012/Harvard/BioDesign/DNA_origami | DNA origami structure]]
<br>
 
The other component of our project was to optimize the design for the L-DNA layer and how to attach it to the template. We also wanted to lower the melting point of the strands so that we could anneal that part on without affecting our pre-formed template.  Here is our work on the [[Biomod/2012/Harvard/BioDesign/L-DNA_layer |L-DNA layer]].

Latest revision as of 23:59, 27 October 2012

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Approaches


Our project had multiple parallel pathways. There were two sub-problems to solve in approaching the templating problem.

One was the templating technique, for which we experimented with three different D-DNA designs;

Small SST canvas
Large SST canvas
DNA origami structure

The other component of our project was to optimize the design for the L-DNA layer and how to attach it to the template. We also wanted to lower the melting point of the strands so that we could anneal that part on without affecting our pre-formed template. Here is our work on the L-DNA layer.

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