Biomod/2012/TU Dresden/Nanosaurs: Difference between revisions
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== Team Meetings == | == Team Meetings == | ||
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=== 24.04.2012 === | === 24.04.2012 === | ||
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*Varsha contacted Prof. Büchner | *Varsha contacted Prof. Büchner | ||
*Thomas wrote to Leonid Ionov (smart polymers) | *Thomas wrote to Leonid Ionov (smart polymers)-> probable meeting next week on Thursday at 5 pm at IPF | ||
*Fereshteh Presented a Nanosaur for a special function (her idea);Environment is key | *Fereshteh Presented a Nanosaur for a special function (her idea);Environment is key -> Low Reynold's number. Reynolds number: Ratio of Inertial term to Viscosity term in Navier-Stokes Eq. Recommended book: Purcell et al: Life at Low Reynolds Number (1976) | ||
*Brainstorm | *Brainstorm-> Control what sth. is doing. ATP is used as source of energy, how can it be supplied in a controlled fashion? At least 3 center of masses are necessary to have a nonreciprocal motion which leads to a pure displacement, at low Reynolds number. Aim: Build a little nano-swimmer to act autonomously in the body. | ||
*Meeting with Dominik Kauert on Thursday at 4:30 pm in his office | *Meeting with Dominik Kauert on Thursday at 4:30 pm in his office | ||
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End: 22:07 | End: 22:07 | ||
=== 26.04.2012 === | === 26.04.2012 === | ||
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*Talk from Mari about MIT project: House shaped Origami -> targeted drug delivery:Roof could open using ph change: I-switch (ph switch).Ground floor can open due to light activation (azobenzene link in cis) -> so the “house” can open due to two different signals on two positions and therefore could have two different chambers in one origami.They evaluated the opening by using FRET measurements. | *Talk from Mari about MIT project: House shaped Origami -> targeted drug delivery:Roof could open using ph change: I-switch (ph switch).Ground floor can open due to light activation (azobenzene link in cis) -> so the “house” can open due to two different signals on two positions and therefore could have two different chambers in one origami.They evaluated the opening by using FRET measurements. | ||
*Ali: DNA spiders -> CalTec: Principle of DNA spider | *Ali: DNA spiders -> CalTec: Principle of DNA spider-> 3 legs attached to body, DNA enzyme legs: they split the DNA single strands into shorter strands. Leaving a “burned” path.Molecular Spiders (paper):http://www.nature.com/nature/journal/v465/n7295/full/nature09012.html#/supplementary-information. Random walk: Pick cargos up and should deliver them to one specific site of the Origami.Nice and interesting project: but the problem is, that it might be not useful to work on a project, where there is no knowledge at TU Dresden.What we could use: Walking mechanism by partly complementary strands (useful to make something move, deliver sth. Idea of Ali to use a Jump mechanism for faster targeted delivery of the loads of the spiders. | ||
*Karen: LMU Munich team: Modular nanoscopic drug delivery device with a ph sensitive opening mechanism (using DNA origami) I-motif is pH dependent: Uses cytosins, at low pH values it can pair to itself. | *Karen: LMU Munich team: Modular nanoscopic drug delivery device with a ph sensitive opening mechanism (using DNA origami) I-motif is pH dependent: Uses cytosins, at low pH values it can pair to itself. | ||
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*G-quadruplex: needs an ion (dependent, metal ion: cation); cancer cells elongate the telomeres by the function of telomerase: a g-quadruplex at the end can prevent the function of the telomerase. | *G-quadruplex: needs an ion (dependent, metal ion: cation); cancer cells elongate the telomeres by the function of telomerase: a g-quadruplex at the end can prevent the function of the telomerase. | ||
*Thomas: Danish Nano artists | *Thomas: Danish Nano artists-> octahedron which can be used as drug delivery system and can itself work as drug by using RNA interference Why did they use RNA for the Origami? Only possibility to use the container itself for RNA interference and knockdown of genes. | ||
*We need more brainstorm sessions before the tenth of Mai….Meeting on Monday: 7th after the lecture in cellular machines. | *We need more brainstorm sessions before the tenth of Mai….Meeting on Monday: 7th after the lecture in cellular machines. | ||
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*Leonid Ionov works with smart polymers-> stimuli responsive polymers and they do a biomimetic approach and even try to do Origami folding techniques with Polymers. | *Leonid Ionov works with smart polymers-> stimuli responsive polymers and they do a biomimetic approach and even try to do Origami folding techniques with Polymers. | ||
*The change in the polymer is mostly due to swelling, how is it possible to engineer the swelling behavior upon specific stimulus | *The change in the polymer is mostly due to swelling, how is it possible to engineer the swelling behavior upon specific stimulus-> In IPF they work with thermoresponsive polymers: | ||
**Temperature low: swelling; | **Temperature low: swelling; | ||
**temperature high: unswelling | **temperature high: unswelling | ||
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*Long Night of Science: DNA Origami for dummies: | *Long Night of Science: DNA Origami for dummies: | ||
*Workshop | *Workshop-> Applications for DNA Nanotechnology: Robots, Rockets, advertising for BioMod competition, create a platform for donation. | ||
*Say what BioMod is,explain also transcription. | *Say what BioMod is,explain also transcription. | ||
*Poster Our project explanation; BioMod; DNA Applications in Nanotechnology: 3 posters | *Poster Our project explanation; BioMod; DNA Applications in Nanotechnology: 3 posters | ||
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Some key points for DNA origami: | Some key points for DNA origami: | ||
*Website: cando | *Website: cando-> Upload DNA Origami, they send a video what the Origami would look like | ||
*Agata send Dominik and Alexander (Olek) an Email to meet them. | *Agata send Dominik and Alexander (Olek) an Email to meet them. | ||
*We already had a GUV lab practical which could be useful. | *We already had a GUV lab practical which could be useful. | ||
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#Then order DNA Origami (1 week) | #Then order DNA Origami (1 week) | ||
#Pipette together-> assemble it (fast routine: slow routine: 15 h : 72 h); heating and cooling down, then the structure can assemble. | #Pipette together-> assemble it (fast routine: slow routine: 15 h : 72 h); heating and cooling down, then the structure can assemble. | ||
#For already proven structures | #For already proven structures-> How long does it take to replicate structure? Usually in the supplementary data you can replicate it around 4 hours (maybe with a good computer: 2 hours); | ||
Label sth. for example with a biotin. Book appointment at TEM. | Label sth. for example with a biotin. Book appointment at TEM. | ||
Basic Idea: | Basic Idea: | ||
*The transcription solution works better for the artificial cell system. Transcription part is ready. Motivation is not good enough. Everything happens outside the cel... | *The transcription solution works better for the artificial cell system. Transcription part is ready. Motivation is not good enough. Everything happens outside the cel... | ||
*The GUV attachment to each other | *The GUV attachment to each other-> Integrin's idea: Mimic one step of vesicle fusion; | ||
#DNA Origami incorporated in the GUVs | #DNA Origami incorporated in the GUVs | ||
#Signal comes -> aptamer approach | #Signal comes -> aptamer approach | ||
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##We need to meet the junior Group-leader (Zhang) in B-cube who can functionalize Origami-staples with cholesterol linkers. | ##We need to meet the junior Group-leader (Zhang) in B-cube who can functionalize Origami-staples with cholesterol linkers. | ||
##Praveen explains about aptamers, we could use them for cancer targeting; | ##Praveen explains about aptamers, we could use them for cancer targeting; | ||
##Things to consider | ##Things to consider-> final result we want to obtain should not interfere with the aptamer (vesicles?) | ||
##Maybe a prism structure for the origami | ##Maybe a prism structure for the origami | ||
#Funding: | #Funding: | ||
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##50% of the final mark, Santiago is working on it | ##50% of the final mark, Santiago is working on it | ||
#Video: | #Video: | ||
##Thomas met Shruti today | ##Thomas met Shruti today-> She might be really helpful do to his media experience. We have already some ideas to catch people s attention. Important is to have a main theme in the video…like stars, water, …. | ||
#GUV Front: | #GUV Front: | ||
##Please ask Aleksander (Olek) about further experimental steps, ask him what we need to order. | ##Please ask Aleksander (Olek) about further experimental steps, ask him what we need to order. | ||
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Time: 17:30 | Time: 17:30 | ||
#Alexander presented the Steps for the experiment | #Alexander presented the Steps for the experiment-> Find presentation in the googlegroup | ||
##We need three different cholesterol labelled oligos | ##We need three different cholesterol labelled oligos | ||
##9 connections to anchor the Origami on the outside of the GUV | ##9 connections to anchor the Origami on the outside of the GUV | ||
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#Regular Meetings: | #Regular Meetings: | ||
Mondays: 5:30 pm; Fridays: 4 pm (before beer Hour, Update of the week!!) | Mondays: 5:30 pm; Fridays: 4 pm (before beer Hour, Update of the week!!) | ||
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== Section2 == | == Section2 == | ||
Revision as of 03:12, 3 August 2012
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Team Meetings
Section2
Section3
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- Team Name:Dresden Nanosaurs
- Institution Name: TU Dresden
- Geographic Location: Dresden, Germany
Welcome to the Wiki of the Dresden Nanosaurs! During the next weeks and months we are going to update this page and post our progress of the project.
"Good, better, best. Never let it rest. 'Til your good is better and your better is best"
