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[[Image:スクリーンショット 2012-10-28 8.27.26.png|center|600px]]
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We decided to divide our project into several subprojects to do experiments in parallel. The sub-projects are GATE, PORTER, and MEMBRANE projects. We also have SIMULATION project to evaluate design of each sub-project.
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GATE is the gatekeeper that allows only the target to enter the cell. Actually, is cylindrical DNA nanostructure connecting the inside and outside of membrane like a channel. Because GATE is made of DNA origami, electric repulsions caused by the negative charge of the DNA backbone prevent not desired DNA from entering GATE.
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PORTER is in charge of the active transporting of the target into GATE. It is composed of single stranded DNA (ssDNA) sequences. Each ssDNA sequence is called Porter. These Porters are designed to transfer target DNA strands into (or out from) the membrane. <html></br></html>
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[[Image:スライド3.jpg|center|400px|thumb|Our strategy is making liposome indeed cell's membrane]]
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<h1>Project</h1>
<img src=" http://openwetware.org/images/c/c8/Format_cell_gate.jpg " alt="hybrid graph" align="left" width="465px" height="315px" >
<p>
私たちは人口チャネルを作りたい。
人工チャネルを作ろうとした場合、それは運びたいものを選択的かつ能動的に運べるものであることが望ましい。また、人間の手で形状や機能を自由に変えられるものが望ましい。これら二点を考慮した時に私たちはDNAを用いてチャネルを作るのはどうかと思い立った。DNAオリガミを用いてチャネルを作ればチャネルの形状は私たちの設計によって自在に変えられる。またチャネル内で目的物を輸送する構造もDNAの相補的な配列を持つものと結合するという特性を利用すれば可能なのではないかと考え、その結果私たちはDNAでチャネルを作ることを決めた。実際に人口チャネルを作るにあたって、私たちはリポソームを細胞膜のモデルとして用いることでチャネルの動作確認を行おうと考えた。つまり今回の実験で私たちが目指すゴールは以下のようなものになる。（ここでＤ－Ｈｅａｒｔの画像）。実験はチャネル本体、チャネルの輸送機構、リポソームの3つに分けて進めていく。（3つ統合の画像）
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We will make the gate made of DNA origami!
Gate is cylindrical structure connecting the inside and outside. And the "Porter" is planted in gate. We can use this as an injector or extractor.


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<h2>Sub-project GOAL</h2>
 
The goal of this sub-project is to attach gate structure on liposomes and observe them by fluorescence microscope.
<h1>Porter</h1>
{{-}}
<p>
If we design that the DNA binds the target more stable than former one, and if next DNA binds more stable than it…, the target moves to most complementary sequence DNA. We thought this characteristic of DNA can be utilized the power of channel. This channel can make us transport the object selectively and actively independent of concentration gradient.  
We make porter made of single stranded DNA!
 
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<h1>Membrane</h1>
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We make liposome by using lipid, and utilize liposome as model of cell-membrane.
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<h1>Application in future</h1>
<p>
最終的には実際に細胞膜に刺して物質の輸送を行うことを目的としている。これが出来れば、医療への応用は勿論の事、細胞内に存在する今までとってくることが難しかったような物質までとってくることが出来るようになるかもしれない。また今回はあくまでモデルとしてリポソームを用いたが、リポソームに刺さったチャネルを一つのロボットとしてみなせば、体内でのお掃除ロボットもしくは薬の散布ロボットのような使い道も考えられる。（将来像の画像あってもいいかも）
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Latest revision as of 19:42, 27 October 2012

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<body> <div id="Container"> <!-- Menu --> <ul id="menu"> <li><a href="http://openwetware.org/wiki/Biomod/2012/Tohoku/Team_Sendai ">Home</a></li> <li><a href=" http://openwetware.org/wiki/Biomod/2012/TeamSendai/Idea ">Project</a></li> <li><a href=" http://openwetware.org/wiki/Biomod/2012/TeamSendai/Design">Design</a> </li> <li><a href=" http://openwetware.org/wiki/Biomod/2012/TeamSendai/Simulation">Simulation</a> </li> <li> <a href=" http://openwetware.org/wiki/Biomod/2012/TeamSendai/Experiment ">Experiment</a> </li> <li> <a href=" http://openwetware.org/wiki/Biomod/2012/TeamSendai/Future Application">Future Application</a> </li> <li> <a href=" http://openwetware.org/wiki/Biomod/2012/TeamSendai/Diary">Diary</a> </li> <li> <a href=" http://openwetware.org/wiki/Biomod/2012/TeamSendai/Team ">Team</a> </li> <li> <a href=" http://openwetware.org/wiki/Biomod/2012/Tohoku/Team Sendai/header"></a> </li>

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Project



We decided to divide our project into several subprojects to do experiments in parallel. The sub-projects are GATE, PORTER, and MEMBRANE projects. We also have SIMULATION project to evaluate design of each sub-project.

<html><h1><a name="GATE">Sub-project GATE</a></h1></html>

Function

GATE is the gatekeeper that allows only the target to enter the cell. Actually, is cylindrical DNA nanostructure connecting the inside and outside of membrane like a channel. Because GATE is made of DNA origami, electric repulsions caused by the negative charge of the DNA backbone prevent not desired DNA from entering GATE. In order to work as an injector (or extractor) a PORTER system is planted inside this cylinder (see next section).


GATE is the gatekeeper that allows only the target to enter the cell.

Sub-project GOAL

The goal of this sub-project is to prove this structure is self-assembled by electrophoresis and AFM

<html><h1><a name="PORTER">Sub-project PORTER</a></h1></html>

Function


PORTER is in charge of the active transporting of the target into GATE. It is composed of single stranded DNA (ssDNA) sequences. Each ssDNA sequence is called Porter. These Porters are designed to transfer target DNA strands into (or out from) the membrane. <html></br></html> The first Porter is likely to be outside GATE because of its electric repulsion. Furthermore, the first Porter catches the target DNA and pull it inside the GATE by hybridizing with it. Inner Porters that have higher affinity than the previous Porter pull the target inside GATE step by step.



PORTER is in charge of the active transporting of the target into GATE.

Sub-project GOAL

The goal of this sub-project is to confirm this Porter system is working by electrophoresis


<html><h1><a name="MEMBRANE">Sub-project MEMBRANE</a></h1></html>

Function

As active transporter, "CELL-GATE" should work in a cell membrane. Thus, a implementation module for inserting it to membranes needs to be designed. DNA sequences with a hydrophobic molecule (cholesterol) are attached outside and around GATE. We use a liposome (artificial lipid vesicle) as a model for the cell membrane.


Our strategy is making liposome indeed cell's membrane


Sub-project GOAL

The goal of this sub-project is to attach gate structure on liposomes and observe them by fluorescence microscope.

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