Biomod/2012/TeamSendai/Idea: Difference between revisions

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<img src=" http://openwetware.org/images/c/c8/Format_cell_gate.jpg " alt="hybrid graph" align="left" width="465px" height="315px" >
<img src=" http://openwetware.org/images/c/c8/Format_cell_gate.jpg " alt="hybrid graph" align="left" width="465px" height="315px" >
<p>
<p>
私たちは人口チャネルを作りたい。
We want to make an artificial channel. If we make an
人工チャネルを作ろうとした場合、それは運びたいものを選択的かつ能動的に運べるものであることが望ましい。また、人間の手で形状や機能を自由に変えられるものが望ましい。これら二点を考慮した時に私たちはDNAを用いてチャネルを作るのはどうかと思い立った。DNAオリガミを用いてチャネルを作ればチャネルの形状は私たちの設計によって自在に変えられる。またチャネル内で目的物を輸送する構造もDNAの相補的な配列を持つものと結合するという特性を利用すれば可能なのではないかと考え、その結果私たちはDNAでチャネルを作ることを決めた。実際に人口チャネルを作るにあたって、私たちはリポソームを細胞膜のモデルとして用いることでチャネルの動作確認を行おうと考えた。つまり今回の実験で私たちが目指すゴールは以下のようなものになる。（ここでＤ－Ｈｅａｒｔの画像）。実験はチャネル本体、チャネルの輸送機構、リポソームの3つに分けて進めていく。（3つ統合の画像）
artificial channel, it is desirable that the channel can
carry selectively and actively only what we want to carry.
Also, it is desirable that the channel can change its
function and shape artificially. Think of these things, we
think it is best to make this structure with DNA-origami.
If we use DNA, the shape of the channel is changeable as we
like. Also, the carry structure that through the channel
is possible by using DNA’s complementarity, so we decided
to use DNA to make the structure. When we test the effect
of the artificial channel, we use liposome as a model of
a cell membrane. In short, our goal in this experiment is
THIS! (画像) Our experiment separates three parts; channel structure part,
carry system part, and liposome part.(画像)
</br>
</br>
</br>
</br>
Line 273: Line 285:
<h1>Application in future</h1>
<h1>Application in future</h1>
<p>
<p>
最終的には実際に細胞膜に刺して物質の輸送を行うことを目的としている。これが出来れば、医療への応用は勿論の事、細胞内に存在する今までとってくることが難しかったような物質までとってくることが出来るようになるかもしれない。また今回はあくまでモデルとしてリポソームを用いたが、リポソームに刺さったチャネルを一つのロボットとしてみなせば、体内でのお掃除ロボットもしくは薬の散布ロボットのような使い道も考えられる。（将来像の画像あってもいいかも）
Finally, this project aims to attach to real cell and
transport a substance to cell and from cell. Of cause,
this channel can be applied to medical use. Also, it can
 
be used for bring some substance which it is difficult
to bring back now from cell. In this experiment, we used
liposome as a model of a cell membrane, but if we consider
the channel attached liposome as one robot, the robot can
use to cleaner robot or medical sprinkling robot.
</p>
</p>
</div>
</div>

Revision as of 01:21, 25 October 2012

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<body> <div id="Container"> <!-- Menu --> <ul id="menu"> <li><a href="http://openwetware.org/wiki/Biomod/2012/Tohoku/Team_Sendai ">Home</a></li> <li><a href=" http://openwetware.org/wiki/Biomod/2012/TeamSendai/Idea ">Project</a></li> <li><a href=" http://openwetware.org/wiki/Biomod/2012/TeamSendai/Design">Design</a> </li> <li><a href=" http://openwetware.org/wiki/Biomod/2012/TeamSendai/Simulation">Simulation</a> </li> <li> <a href=" http://openwetware.org/wiki/Biomod/2012/TeamSendai/Experiment ">Experiment</a> </li> <li> <a href=" http://openwetware.org/wiki/Biomod/2012/TeamSendai/Future Application">Future Application</a> </li> <li> <a href=" http://openwetware.org/wiki/Biomod/2012/TeamSendai/Diary">Diary</a> </li> <li> <a href=" http://openwetware.org/wiki/Biomod/2012/TeamSendai/Team ">Team</a> </li> <li> <a href=" http://openwetware.org/wiki/Biomod/2012/Tohoku/Team Sendai/header"></a> </li>

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<h1>Project</h1> <img src=" http://openwetware.org/images/c/c8/Format_cell_gate.jpg " alt="hybrid graph" align="left" width="465px" height="315px" > <p> We want to make an artificial channel. If we make an artificial channel, it is desirable that the channel can carry selectively and actively only what we want to carry. Also, it is desirable that the channel can change its function and shape artificially. Think of these things, we think it is best to make this structure with DNA-origami. If we use DNA, the shape of the channel is changeable as we like. Also, the carry structure that through the channel is possible by using DNA’s complementarity, so we decided to use DNA to make the structure. When we test the effect of the artificial channel, we use liposome as a model of a cell membrane. In short, our goal in this experiment is THIS! (画像) Our experiment separates three parts; channel structure part, carry system part, and liposome part.(画像) </br> </br> </br> </br> </br> </br> </br> </br> </p>

<h1>Gate</h1> <p> We will make the gate made of DNA origami! Gate is cylindrical structure connecting the inside and outside. And the "Porter" is planted in gate. We can use this as an injector or extractor.

</p>

<h1>Porter</h1> <p> If we design that the DNA binds the target more stable than former one, and if next DNA binds more stable than it…, the target moves to most complementary sequence DNA. We thought this characteristic of DNA can be utilized the power of channel. This channel can make us transport the object selectively and actively independent of concentration gradient. We make porter made of single stranded DNA!

<br clear="left"> </p>

<h1>Membrane</h1> <p> We make liposome by using lipid, and utilize liposome as model of cell-membrane. </p>

<br clear="left"> </p>

<h1>Application in future</h1> <p> Finally, this project aims to attach to real cell and transport a substance to cell and from cell. Of cause, this channel can be applied to medical use. Also, it can

be used for bring some substance which it is difficult to bring back now from cell. In this experiment, we used liposome as a model of a cell membrane, but if we consider the channel attached liposome as one robot, the robot can use to cleaner robot or medical sprinkling robot. </p> </div>

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