So we decided to divide our project into several subprojects to do experiments in parallel.
The subprojects are GATE, PORTER, and MEMBRANE projects. We have also SIMULATION project to evaluate design of each subprojects.
GATE is the gatekeeper that allows only the target to enter the cell.
Electric repulsions caused from the negative charge of the DNA backbone inhibit NOT desired DNA from entering GATE.
To realize PORTER is in charge of the active transporting of the target into GATE.
The first Porter is likely to be outside GATE because of its electric repulsion. Furthermore, the first Porter catches the target DNA and pull it inside the GATE by hybridizing with it.
Inner Porters that have higher affinity than the first Porter pull the target inside GATE step by step.
As active transporter, "CELL-GATE" should work in a cell membrane. Thus, a implementation module for inserting it to membranes needs to be designed. DNA sequences with a hydrophobic molecule (cholesterol) are attached outside and around GATE. We use a liposome (artificial lipid vesicle) as a model cell membrane.