Biomod/2012/TeamSendaiA/Project: Difference between revisions
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We think on three bonding methods: "Using cationic lipid as adhesive", "Using sticky ends growing from origami" and "Using cationic lipid and sticky ends".</br> | We think on three bonding methods: "Using cationic lipid as adhesive", "Using sticky ends growing from origami" and "Using cationic lipid and sticky ends".</br> | ||
We think "Using cationic lipid as adhesive" is the best method among the three because there is no need of annealing-time. | We think "Using cationic lipid as adhesive" is the best method among the three because there is no need of annealing-time. | ||
In addition cationic lipid will reduce the force of repulsion of the negatively charged DNA. Cationic lipid will enable us to make huge DNA | In addition cationic lipid will reduce the force of repulsion of the negatively charged DNA. Cationic lipid will enable us to make huge DNA structures in other words multi-functional mother cubes. | ||
</p> | </p> | ||
Revision as of 20:29, 2 September 2012
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<!-- ヘッダー --> <div id="Header"> <h1><img src="http://openwetware.org/images/3/35/Formattop2.jpg " alt="Mother Cube2" "width="800" height="141"//></h1> <p> </p> </div>
<!-- パンくずリスト --> <p id="Bread"> <a href="http://openwetware.org/wiki/Biomod/2012/Tohoku/Team_Sendai ">Team Sendai</a> > <a href=" http://openwetware.org/wiki/Biomod/2012/TeamSendaiA/Top ">Team Sendai A Top</a>> <a href=" http://openwetware.org/wiki/Biomod/2012/TeamSendaiA/Project">Project</a> </p>
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<h2>Project</h2>
<p> In order to create the Mother Cube, we need first to explore the method for parallel bonding DNA origami planes.</br> We think on three bonding methods: "Using cationic lipid as adhesive", "Using sticky ends growing from origami" and "Using cationic lipid and sticky ends".</br> We think "Using cationic lipid as adhesive" is the best method among the three because there is no need of annealing-time. In addition cationic lipid will reduce the force of repulsion of the negatively charged DNA. Cationic lipid will enable us to make huge DNA structures in other words multi-functional mother cubes. </p>
<p><img src="http://openwetware.org/images/5/51/Stage1.jpg" alt="stage1" "width="580" height="23"/></p>
<p> Studying the interaction between lipid and DNA origami, and creating the following shapes with cadnano. </p>
<p> <a href="http://openwetware.org/wiki/BIOMOD/2012/Team_Sendai_A/CADNANO " target="_blank"> <img src="http://openwetware.org/images/f/f1/Planeshape.jpg" alt="plane shape" border="0" "width="580" height="185"/></a> </p>
<p> <em>If you click this image, you get the DNA sequences.</em> </p> <p><img src="http://openwetware.org/images/0/0f/Stage2.jpg" alt="stage2" "width="580" height="27"/></p> <p> We have three ideas about what method to follow in order to bond a plane to other DNA origami plane. We are looking for the best method. </p> <p><img src="http://openwetware.org/images/2/20/Method.jpg " alt="bond method" "width="580" height="517"/></p> <p><img src="http://openwetware.org/images/b/b9/Stage3.jpg " alt="stage3" "width="580" height="27"/></p> <p> Creating the cubes. Loading function and bonding them. <p><img src="http://openwetware.org/images/c/cb/Hako_drug.jpg" alt="stage2" "width="580" height="320"/></p> <p>
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<ul class="sidemenu"> <li><a href="http://openwetware.org/wiki/Biomod/2012/TeamSendaiA/Top">Top</a></li> <li><a href="http://openwetware.org/wiki/Biomod/2012/TeamSendaiA/Project">Project</a></li> <li><a href="http://openwetware.org/wiki/Biomod/2012/TeamSendaiA/caDNAnoWorks">caDNAno Works</a></li> <li><a href="http://openwetware.org/wiki/Biomod/2012/TeamSendaiA/Results_%26_Discussion" >Results & Discussion</a></li> <li><a href="http://openwetware.org/wiki/Biomod/2012/TeamSendaiA/Methods">Methods</a></li> <li><a href="http://openwetware.org/wiki/Biomod/2012/TeamSendaiA/Movie">Movie</a></li> <li><a href="http://openwetware.org/wiki/Biomod/2012/TeamSendaiA/Member">Member</a></li> </ul>
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<address>Copyright (C) Team Sendai A, All rights reserved.</address>
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